REGULATION OF SPERM EXOCYTOSIS

精子胞吐作用的调节

• 批准号: 2203115
• 负责人: NICHOLAS L CROSS
• 金额: $ 11.02万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-19 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2203115
• 关键词: acid base balance; acrosome; affinity chromatography; calcium; cell adhesion molecules; cell membrane; cholesterol; exocytosis; fluorescence microscopy; gas chromatography mass spectrometry; high performance liquid chromatography; human subject; integrins; intracellular; male; membrane fusion; peptides; plasma; secretion; sperm; ultracentrifugation; vesicle /vacuole

The acrosome of a sperm must undergo exocytosis before the sperm fertilizes an egg. Freshly ejaculated mammalian sperm are not capable of exocytosis. They must first complete a poorly understood maturation process before they become acrosomally responsive. The long-term objective of this project is to understand the mechanisms by which exocytosis is controlled. Seminal plasma prevents sperm from becoming acrosomally responsive. The agents in seminal plasma that affect acrosomal responsiveness will be identified. This project will use seminal plasma and molecules isolated from it as tools to analyze the control of acrosomal responsiveness. Depending on how it is applied to sperm, seminal plasma can inhibit the development of acrosomal responsiveness, reverse acrosomal responsiveness, or augment acrosomal responsiveness. Because acrosomal exocytosis is triggered by an increased concentration of intracellular free Ca2+ and may require a rise in intracellular pH, the effect of seminal plasma on the intracellular concentrations of Ca2+ and H+ will be determined. The effect of SP on the fusibility of purified acrosomal and plasma membranes will also be tested. The rate at which human sperm become acrosomally responsive varies among men. Sperm of some men do not become responsive at a normal rate. This behavior is correlated with male subfertility, suggesting that inappropriate acrosomal exocytosis may contribute to male subfertility. A full understanding of the mechanisms by which acrosomal exocytosis is controlled will provide new insights into this class of human subfertility.

精子的高级大精子必须在精子之前患上胞吐作用 施肥鸡蛋。 新鲜射精的哺乳动物精子无法 胞吞作用。他们必须首先完成一个知之甚少的成熟 在它们变得响应质量之前的过程。长期目标 这个项目的是了解胞吐作用的机制 受控。 精确的血浆可防止精子变为杂色的反应性。 这 影响谷子反应能力的精确血浆中的代理将是 确定。该项目将使用精确的等离子体和分离的分子 从中作为分析控制质体响应能力的工具。 根据如何将其应用于精子，精确血浆可以抑制 谷子响应能力的发展，反向杂色响应能力， 或增强杂色的响应能力。因为蛇形胞吐作用是 由细胞内游离Ca2+浓度增加而触发 需要升高细胞内pH，精确血浆对 将确定Ca2+和H+的细胞内浓度。效果 SP关于纯化的Aromal和质膜的合并性 也可以测试。 人类精子变为杂色的响应速度在 男人。一些男人的精子不会以正常的速度反应。这 行为与男性的副不动性相关，表明 不适当的教症胞吐作用可能有助于男性的亚生物。一个 完全了解教诊断胞吐作用的机制 受控将为这类人提供新的见解 亚属。