REGULATION OF SPERM EXOCYTOSIS

精子胞吐作用的调节

• 批准号: 2203114
• 负责人: NICHOLAS L CROSS
• 金额: $ 10.6万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-19 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2203114
• 关键词: acid base balance; acrosome; affinity chromatography; calcium; cell adhesion molecules; cell membrane; cholesterol; exocytosis; fluorescence microscopy; gas chromatography mass spectrometry; high performance liquid chromatography; human subject; integrins; intracellular; male; membrane fusion; peptides; plasma; secretion; sperm; ultracentrifugation; vesicle /vacuole

The acrosome of a sperm must undergo exocytosis before the sperm fertilizes an egg. Freshly ejaculated mammalian sperm are not capable of exocytosis. They must first complete a poorly understood maturation process before they become acrosomally responsive. The long-term objective of this project is to understand the mechanisms by which exocytosis is controlled. Seminal plasma prevents sperm from becoming acrosomally responsive. The agents in seminal plasma that affect acrosomal responsiveness will be identified. This project will use seminal plasma and molecules isolated from it as tools to analyze the control of acrosomal responsiveness. Depending on how it is applied to sperm, seminal plasma can inhibit the development of acrosomal responsiveness, reverse acrosomal responsiveness, or augment acrosomal responsiveness. Because acrosomal exocytosis is triggered by an increased concentration of intracellular free Ca2+ and may require a rise in intracellular pH, the effect of seminal plasma on the intracellular concentrations of Ca2+ and H+ will be determined. The effect of SP on the fusibility of purified acrosomal and plasma membranes will also be tested. The rate at which human sperm become acrosomally responsive varies among men. Sperm of some men do not become responsive at a normal rate. This behavior is correlated with male subfertility, suggesting that inappropriate acrosomal exocytosis may contribute to male subfertility. A full understanding of the mechanisms by which acrosomal exocytosis is controlled will provide new insights into this class of human subfertility.

精子的顶体必须在精子形成之前进行胞吐作用 使卵子受精。 刚射出的哺乳动物精子不能 胞吐作用。他们必须首先完成一个鲜为人知的成熟过程 在它们变得顶体反应之前的过程。长期目标 该项目的目的是了解胞吐作用的机制 受控。 精浆可防止精子产生顶体反应。 这 精浆中影响顶体反应性的物质是 确定。该项目将使用精浆和分离的分子 将其作为分析顶体反应控制的工具。 根据其应用于精子的方式，精浆可以抑制 顶体反应性的发展，逆转顶体反应性， 或增强顶体反应性。因为顶体胞吐作用是 由细胞内游离 Ca2+ 浓度增加引发，可能 需要升高细胞内pH值，精浆对 将测定细胞内 Ca2+ 和 H+ 的浓度。效果 SP 对纯化顶体膜和质膜融合性的影响 也进行测试。 人类精子对顶体反应的速度因人而异 男人。有些男性的精子不会以正常速度产生反应。这 行为与男性生育力低下相关，表明 不适当的顶体胞吐作用可能导致男性生育力低下。一个 充分了解顶体胞吐作用的机制 受控将为此类人类提供新的见解 生育能力低下。