CHARACTERIZATION OF HORMONE-CONCENTRATING NEURONS

激素浓缩神经元的表征

• 批准号: 2198734
• 负责人: Joan Irene Morrell
• 金额: $ 15.58万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2198734
• 关键词: afferent nerve; behavior test; central neural pathway /tract; efferent nerve; estrogen receptors; excitatory aminoacid; female; genetic transcription; genetic translation; ibotenate; immunocytochemistry; in situ hybridization; laboratory rat; limbic system; maternal behavior; messenger RNA; neural information processing; neuroanatomy; neuroendocrine system; neurons; neurotoxins; northern blottings; pregnancy; preoptic areas; progesterone receptors; tetrodotoxin

This proposal will focus on the role of estrogen receptor (ER) and progesterone receptor (PR) containing neurons in the onset of maternal behavior and the attendent neuroendocrine events in the maternally responsive female rat. Specific Aim I- Does pregnancy alter the brain's sensitivity to steroids by induction of ER and PR in the preoptic area (POA) and limbic system? Are these alterations at the transcriptional or translational level? We will test whether the number and distribution of POA and limbic neurons that contain ER and PR is altered during pregnancy and if the amount of receptor protein or mRNA per neuron is altered. Immunocytochemistry (ICC) with ER and PR antibodies or in situ hybridization (or Northern blot) with probes to ER and PR mRNA will be used. Specific Aim II- Is the brain's sensitivity to estrogen and progestin channeled through particular neural circuits that underlie specific components of maternal responsiveness? Do unique efferent projections of ER and PR neurons underlie the different components of the maternal response including altered responsivity to chemosensory inputs, the motor components related to nest building and retrieval, and events related to nursing including crouching, licking and oxytocin release? These experiments will combine retrograde neuroanatomical tracing with ICC detection of ER and PR. Will lesions of the specific populations of ER and PR POA neurons that project to different functional regions alter specific components of the maternal response? Neurotoxins will be used to lesion specific subsets of preoptic neurons that will be defined by their unique efferent projections and/or pregnancy altered concentration of receptors ; behavioral impairment will be tested. Specific Aim III- ER and PR in neuronal populations important for maternal responsiveness could be induced by a number of the important physiological events that occur during the natural course of pregnancy. These experiments will test whether the afferent input to POA alters the induction of ER and PR in the POA during pregnancy. Tetrodotoxin or neurotransmitter antagonists will be used to transiently eliminate afferent input to these neurons; lesion of the chemosensory input carried out. Immunoreactive receptor protein or mRNA content and behavioral results will be examined.

该提案将重点关注雌激素受体（ER）和 孕激素受体（PR）含有母体发病时的神经元 母亲的行为和随之而来的神经内分泌事件 反应灵敏的雌性大鼠。 具体目标 I-怀孕是否会改变大脑的 通过在视前区诱导 ER 和 PR 来提高对类固醇的敏感性 （POA）和边缘系统？这些改变是转录还是 翻译水平？我们将测试是否有数量和分布 POA 和含有 ER 和 PR 的边缘神经元在怀孕期间发生改变 每个神经元的受体蛋白或 mRNA 的量是否发生改变。 使用 ER 和 PR 抗体或原位免疫细胞化学 (ICC) 与 ER 和 PR mRNA 探针杂交（或 Northern 印迹）将 用过的。 具体目标 II - 大脑对雌激素的敏感性和 孕激素通过特定的神经回路传导 母亲反应的具体组成部分？做独特的传出 ER 和 PR 神经元的投射是不同组成部分的基础 母体反应，包括对化学感应输入的反应性改变， 与筑巢和取回以及事件相关的运动组件 与哺乳有关的行为包括蹲伏、舔舐和催产素释放？ 这些实验将逆行神经解剖学追踪与 ER和PR的ICC检测。 特定人群的病变会不会 投射到不同功能区域的 ER 和 PR POA 神经元发生变化 母亲反应的具体组成部分？神经毒素将被用来 视前神经元的病变特异性子集将由其定义 独特的传出预测和/或怀孕改变的浓度 受体；将测试行为障碍。 具体目标 III-ER 神经元群中的 PR 和 PR 对母亲的反应很重要 可能是由许多重要的生理事件引起的 发生在怀孕的自然过程中。 这些实验将 测试 POA 的传入输入是否改变 ER 和 PR 的诱导 怀孕期间的 POA 中。 河豚毒素或神经递质 拮抗剂将用于暂时消除对这些的传入输入 神经元；进行化学感应输入的损伤。 免疫反应性 将检查受体蛋白或 mRNA 含量和行为结果。