Integrative Structural and Functional Characterization of Tip-Link Cadherins Deafness

Tip-Link 钙粘蛋白耳聋的综合结构和功能表征

基本信息

批准号：
10359738
负责人：
Ulrich Mueller
金额：
$ 64.77万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-03-15 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10359738
关键词：
3-Dimensional Adhesions Affect Age Attention Auditory Bacteria Binding Biochemical Biophysics CDH23 gene Cadherin Domain Cadherins Cell physiology Cells Child Cochlea Complex Cryoelectron Microscopy Crystallography Data Defect Development Dimerization Disease Disease Outcome Dissection Extracellular Domain Gene Mutation Genes Genetic Polymorphism Genetically Engineered Mouse Goals Hair Hair Cells Health Hearing Hearing Tests Image Imaging Device Impairment Inner Hair Cells Investigation Ion Channel Laboratories Labyrinth Lead Link Mammalian Cell Maps Mechanics Mediating Membrane Molecular Morphogenesis Mus Mutation Neurosciences Noise Noise-Induced Hearing Loss PCDH15 gene Pathogenesis Physiological Presbycusis Property Publishing Resolution Retina Role Signal Transduction Structure Structure-Activity Relationship Surface Syndrome Testing Usher Syndrome Visual X-Ray Crystallography age related aging population base biochemical tools biophysical analysis congenital deafness deafness dimer early onset extracellular hearing impairment insight intermolecular interaction link protein loss of function mutation mechanotransduction member mutant neurotransmission particle progressive hearing loss protein complex protein structure reconstruction sound vibration

项目摘要

Deafness is a major health problem. A major cause of deafness is defects in hair cells, the mechanosensory cells of the cochlea that convert sound induced vibrations into electrical signals to provide our sense of hearing. Mutations in the genes encoding protocadherin (PCDH15) and cadherin 23 (CDH23) cause hearing loss. Both genes are expressed in the hair bundles of the mechanosensory hair cells of the inner ear where they form heterophilic adhesion complexes that are important for hair bundle morphogenesis and mechanotransduction. Significantly, different mutation in both PCDH15 and CDH23 lead to different disease outcomes. While some mutations cause profound congenital deafness with retinal impairment (Usher Syndrome) others lead to recessive and progressive hearing loss without visual involvement. Gene-association studies also suggest a link of CDH23 polymorphisms with age- and noise-induce hearing loss. The mechanisms by which different mutations lead to distinct disease outcomes are poorly defined. We propose here to combine high-resolution structural studies with functional studies in hair cells to gain insights into the mechanisms by which PCDH15 and CDH23 regulate hair cell function and to define disease mechanisms. To achieve this goal, a laboratory with expertise in studying the biophysical and structural properties of cadherins and a laboratory dedicated to the study of auditory neuroscience have combined their efforts to achieve what either could not accomplish alone. Unlike previous studies that have focused on structural analysis of small monomeric fragments of CDH23 and PCDH15 expressed in bacteria, the team proposed to define the high- resolution structure of natively assembled PCDH15-CDH23 complexes using crystallography and cryo-EM. Structural data will be validated biochemically and by functional interrogation of mutant cadherins in the physiologically relevant mechanosensory hair cells paying attention to mutations associated with disease. We anticipate that our studies will provide the first high-resolution native structure of any protein complex important for mechanotransduction and provide mechanistic insights into its functional properties and pathophysiological mechanisms that are associated with different forms of hearing impairment.

耳聋是一个主要的健康问题。耳聋的一个主要原因是毛细胞（机械感觉细胞）的缺陷耳蜗细胞将声音引起的振动转化为电信号，以提供我们的感觉听力。编码原钙粘蛋白 (PCDH15) 和钙粘蛋白 23 (CDH23) 的基因突变会导致听力下降损失。这两个基因均在内耳机械感觉毛细胞的发束中表达，其中它们形成对发束形态发生很重要的异嗜性粘附复合物力传导。值得注意的是，PCDH15和CDH23的不同突变会导致不同的疾病结果。虽然一些突变会导致严重的先天性耳聋和视网膜损伤（Usher 综合症）其他导致隐性和进行性听力损失，但不涉及视力。基因关联研究还表明 CDH23 多态性与年龄和噪音引起的听力损失有关。这不同突变导致不同疾病结果的机制尚不清楚。我们建议在这里将毛细胞的高分辨率结构研究与功能研究结合起来，以深入了解毛细胞 PCDH15 和 CDH23 调节毛细胞功能的机制并确定疾病机制。到为了实现这一目标，我们建立了一个专门研究钙粘蛋白生物物理和结构特性的实验室和一个致力于听觉神经科学研究的实验室共同努力，以实现以下目标：任何一个都无法单独完成。与以前的研究不同，这些研究侧重于小型结构分析为了研究细菌中表达的 CDH23 和 PCDH15 的单体片段，研究小组提出定义高使用晶体学和冷冻电镜解析天然组装的 PCDH15-CDH23 复合物的结构。结构数据将通过生化和突变钙粘蛋白的功能询问进行验证生理相关的机械感觉毛细胞关注与疾病相关的突变。我们预计我们的研究将提供任何重要蛋白质复合物的第一个高分辨率天然结构用于机械转导并提供对其功能特性和病理生理学的机制见解与不同形式的听力障碍相关的机制。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Bayesian Adaptive Beamformer for Robust Electromagnetic Brain Imaging of Correlated Sources in High Spatial Resolution.

用于高空间分辨率相关源的鲁棒电磁脑成像的贝叶斯自适应波束形成器。

DOI：
发表时间：
2023-09
期刊：
影响因子：
10.6
作者：
Cai, Chang;Long, Yuanshun;Ghosh, Sanjay;Hashemi, Ali;Gao, Yijing;Diwakar, Mithun;Haufe, Stefan;Sekihara, Kensuke;Wu, Wei;Nagarajan, Srikantan S
通讯作者：
Nagarajan, Srikantan S

Elasticity of individual protocadherin 15 molecules implicates tip links as the gating springs for hearing.

单个原钙粘蛋白 15 分子的弹性表明尖端连接是听力的门控弹簧。

DOI：
发表时间：
2019
期刊：
影响因子：
11.1
作者：
Bartsch, Tobias F;Hengel, Felicitas E;Oswald, Aaron;Dionne, Gilman;Chipendo, Iris V;Mangat, Simranjit S;El Shatanofy, Muhammad;Shapiro, Lawrence;Müller, Ulrich;Hudspeth, A J
通讯作者：
Hudspeth, A J

A joint subspace mapping between structural and functional brain connectomes.

结构性和功能性大脑连接体之间的联合子空间映射。

DOI：
发表时间：
2023-05-15
期刊：
影响因子：
5.7
作者：
Ghosh, Sanjay;Raj, Ashish;Nagarajan, Srikantan S
通讯作者：
Nagarajan, Srikantan S

The Visual Word Form Area compensates for auditory working memory dysfunction in schizophrenia.

视觉词形区可以补偿精神分裂症患者的听觉工作记忆功能障碍。

DOI：
发表时间：
2020-06-01
期刊：
影响因子：
4.6
作者：
Herman, Alexander B;Brown, Ethan G;Dale, Corby L;Hinkley, Leighton B;Subramaniam, Karuna;Houde, John F;Fisher, Melissa;Vinogradov, Sophia;Nagarajan, Srikantan S
通讯作者：
Nagarajan, Srikantan S

Adhesion Protein Structure, Molecular Affinities, and Principles of Cell-Cell Recognition.

粘附蛋白结构、分子亲和力和细胞间识别原理。