ENHANCING THE MOUSE RESOURCE RECOMBINANT INBRED STRAINS

增强小鼠资源重组近交系

• 批准号: 2283303
• 负责人: Beverly J Paigen
• 金额: $ 16.05万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283303
• 关键词: alleles; animal breeding; animal colony; animal population genetics; atherosclerosis; biomedical facility; cholelithiasis; computer program /software; diabetes mellitus; dietary lipid; embryo /fetus; embryo /fetus preservation; freezing; genetic mapping; genetic markers; genetic registry /resource /referral center; genetic strain; inbreeding; information systems; laboratory mouse; linkage mapping; nutrition related tag; obesity; publications; recombinant DNA; restriction fragment length polymorphism; southern blotting

Recombinant inbred (RI) strains are a powerful tool for the analysis and mapping of complex traits of biomedical significance. The largest existing set of RI strains, a 45-strain set derived from C57BL/6J and A/J, is in danger of being lost. These strains are of special importance to biomedical research because the progenitors differ in atherosclerosis, diabetes, obesity, gall stone formation, cancer, birth defects, and susceptibility to over 30 infectious diseases including herpes, leprosy, and tuberculosis. We propose to import this animal genetic resource into The Jackson Laboratory, to permanently preserve each strain by freezing 1,000 embryos, and to provide mice to other investigators. To facilitate the use of these RI strains, we will distribute DNA from each strain, Southern blots of progenitor DNA cut with 10 restriction enzymes to aid in the search for polymorphisms and Southern blots of DNA from the entire RI set to facilitate the search for linkage. Although this is primarily a resource grant, we will characterize this RI set further by analyzing genetic differences between the progenitors in diseases induced by a high fat diet, such as atherosclerosis, obesity, diabetes, and gall stone formation. We will type additional strategically located DNA markers in this RI set so that no genetic marker is more than 20 cM from another. When this goal is accomplished, this RI resource can be used to rapidly and efficiently map any new gene polymorphic by simply determining the strain distribution of the alleles among the RI strains, between the progenitors. We propose an Informatics Center that will include all RI strain sets. The components of this Informatics Center will be a Macintosh computer program, RI Manager, readily available to all investigators, continuously updated strain distribution patterns from the current literature available on computer disk, and semiannual publication of a booklet with the strain distribution patterns of all RI strain sets, similar to the information booklets currently released periodically by The Jackson Laboratory on human-mouse homologies, mouse DNA probes, and the mouse genetic map.

重组近交 (RI) 菌株是分析和鉴定菌株的有力工具。 绘制具有生物医学意义的复杂特征。 最大 现有的 RI 菌株组、源自 C57BL/6J 的 45 个菌株组和 A/J，有丢失的危险。 这些菌株具有特别重要的意义 生物医学研究，因为祖细胞在动脉粥样硬化方面有所不同， 糖尿病、肥胖、胆结石形成、癌症、出生缺陷和 易患 30 多种传染病，包括疱疹、麻风病、 和结核病。 我们建议将这种动物遗传资源导入 杰克逊实验室，通过冷冻永久保存每种菌株 1,000 个胚胎，并向其他研究人员提供小鼠。 为了方便 使用这些 RI 菌株，我们将分配每个菌株的 DNA， 用 10 种限制性内切酶切割祖代 DNA 的 Southern 印迹以帮助 寻找整个 DNA 的多态性和 Southern 印迹 注册机设置方便搜索联动。 虽然这主要是 资源拨款，我们将通过分析进一步描述该 RI 集的特征 高浓度引起的疾病中祖细胞之间的遗传差异 脂肪饮食，如动脉粥样硬化、肥胖、糖尿病和胆结石 形成。 我们将在其中键入其他具有战略意义的 DNA 标记 此 RI 设定使得遗传标记与另一个遗传标记之间的距离不超过 20 cM。 当这一目标实现时，该 RI 资源可用于快速 并通过简单地确定任何新的基因多态性来有效地定位 RI 菌株之间等位基因的菌株分布 祖先。 我们提议建立一个信息学中心，其中将包括所有 RI 菌株集。 该信息中心的组件将是一台 Macintosh 计算机 程序、RI 管理器，可供所有研究人员持续使用 根据当前文献更新应变分布模式 可以在计算机磁盘上找到，并且半年出版一本小册子，其中包含 所有 RI 应变集的应变分布模式，类似于 杰克逊目前定期发布的信息手册 人鼠同源性、小鼠 DNA 探针和小鼠实验室 遗传图谱。