ENHANCING THE MOUSE RESOURCE RECOMBINANT INBRED STRAINS

增强小鼠资源重组近交系

• 批准号: 2283303
• 负责人: Beverly J Paigen
• 金额: $ 16.05万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283303
• 关键词: alleles; animal breeding; animal colony; animal population genetics; atherosclerosis; biomedical facility; cholelithiasis; computer program /software; diabetes mellitus; dietary lipid; embryo /fetus; embryo /fetus preservation; freezing; genetic mapping; genetic markers; genetic registry /resource /referral center; genetic strain; inbreeding; information systems; laboratory mouse; linkage mapping; nutrition related tag; obesity; publications; recombinant DNA; restriction fragment length polymorphism; southern blotting

Recombinant inbred (RI) strains are a powerful tool for the analysis and mapping of complex traits of biomedical significance. The largest existing set of RI strains, a 45-strain set derived from C57BL/6J and A/J, is in danger of being lost. These strains are of special importance to biomedical research because the progenitors differ in atherosclerosis, diabetes, obesity, gall stone formation, cancer, birth defects, and susceptibility to over 30 infectious diseases including herpes, leprosy, and tuberculosis. We propose to import this animal genetic resource into The Jackson Laboratory, to permanently preserve each strain by freezing 1,000 embryos, and to provide mice to other investigators. To facilitate the use of these RI strains, we will distribute DNA from each strain, Southern blots of progenitor DNA cut with 10 restriction enzymes to aid in the search for polymorphisms and Southern blots of DNA from the entire RI set to facilitate the search for linkage. Although this is primarily a resource grant, we will characterize this RI set further by analyzing genetic differences between the progenitors in diseases induced by a high fat diet, such as atherosclerosis, obesity, diabetes, and gall stone formation. We will type additional strategically located DNA markers in this RI set so that no genetic marker is more than 20 cM from another. When this goal is accomplished, this RI resource can be used to rapidly and efficiently map any new gene polymorphic by simply determining the strain distribution of the alleles among the RI strains, between the progenitors. We propose an Informatics Center that will include all RI strain sets. The components of this Informatics Center will be a Macintosh computer program, RI Manager, readily available to all investigators, continuously updated strain distribution patterns from the current literature available on computer disk, and semiannual publication of a booklet with the strain distribution patterns of all RI strain sets, similar to the information booklets currently released periodically by The Jackson Laboratory on human-mouse homologies, mouse DNA probes, and the mouse genetic map.

重组近交（RI）菌株是分析的强大工具， 生物医学意义的复杂性状的映射。 最大 现有的RI菌株集，这是一个从C57BL/6J派生的45支菌株和 A/J，有丢失的危险。 这些菌株特别重要 生物医学研究是因为祖细胞在动脉粥样硬化中有所不同， 糖尿病，肥胖，胆结石形成，癌症，先天缺陷和 对30多种传染病的敏感性，包括疱疹，麻风病， 和结核病。 我们建议将这种动物遗传资源导入 杰克逊实验室，通过冷冻永久保留每种应变 1,000个胚胎，并向其他研究人员提供小鼠。 促进 使用这些RI菌株，我们将从每个菌株中分布DNA， 用10个限制酶切割的祖细胞DNA的南部印迹以帮助 在寻找整个DNA的多态性和南部印迹中 RI设置以方便搜索链接。 虽然这主要是 资源赠款，我们将通过分析进一步表征此RI设置 疾病中祖细胞之间的遗传差异 脂肪饮食，例如动脉粥样硬化，肥胖，糖尿病和胆结石 形成。 我们将在 此RI设置，使得与另一个遗传标记不超过20厘米。 实现此目标后，该RI资源可用于快速 并通过简单地确定 RI菌株中等位基因的应变分布 祖先。 我们提出了一个信息中心，其中包括所有RI菌株集。 该信息中心的组件将是一台Macintosh计算机 RI经理计划，所有调查人员都可以不断地使用 当前文献更新的应变分布模式 可在计算机磁盘上可用，并与 所有RI应变集的应变分布模式，类似于 杰克逊目前定期发布的信息手册 人鼠同源物，小鼠DNA探针和小鼠实验室 遗传图。