MHC Genetic Typing Core

MHC 基因分型核心

• 批准号: 10440879
• 负责人: Eric J. Vallender
• 金额: $ 7.92万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10440879
• 关键词: AIDS/HIV problem; Acceleration; Acquired Immunodeficiency Syndrome; Alleles; Animal Husbandry; Animals; Behavioral Genetics; Breeding; Budgets; Collaborations; Communities; DNA Library; Data; Databases; Development; Disease Progression; Ensure; Funding; Future; Genetic; Genetic Services; Genetic Variation; Genome; Genomics; Genotype; Geographic Locations; Geography; Goals; Grant; Haplotypes; Health; Immune response; Inbreeding; Infection; Informatics; Institution; MHC Class I Genes; Macaca mulatta; Maintenance; Mind; Monitor; Other Genetics; Output; Population; Predisposition; Preventive Medicine; Primates; Process; Protocols documentation; Research; Research Activity; Research Personnel; Role; SIV; Scientist; Services; Source; Structure; System; Targeted Research; Testing; Time; Translational Research; Wisconsin; animal colony; animal data; base; data sharing; deep sequencing; design; evidence base; exhaustion; exome sequencing; experience; genetic information; genetic pedigree; genetic resource; genetic testing; genome sequencing; genomic data; genomic variation; improved; male; nonhuman primate; programs; reproductive; research study; simian human immunodeficiency virus; testing services; tool; whole genome; working group

Project Summary/Abstract - MHC Genetic Typing Core The SPF rhesus macaque breeding colony for AIDS research at the Tulane National Primate Research Center (TNPRC) is part of an extensive evidence-based, integrative, program that incorporates best practices in health surveillance, husbandry, preventive medicine, and behavioral and genetic management. The goals of the genetic management program are to 1) maintain genetic diversity and minimize inbreeding to support sustainable populations and 2) characterize class I MHC alleles in support of HIV/AIDS research activities. To this end, the TNPRC Genome Bank collects and stores materials from more than 9000 unique animals, with additional animals collected as they are produced. The colony is characterized for ancestral geographic-origin and parentage confirmed using a pan-NPRC SNP panel. MHC typing for all animals includes testing for 10 class I alleles, which serves the needs of most core and affiliate scientists, and MHC deep sequencing in collaboration with the Wisconsin National Primate Research Center for targeted research programs. Over the previous grant funding period whole genome sequencing (WGS) has been added for 164 key breeders, focusing on those males with the greatest reproductive output in the breeding colony, and whole exome sequencing (WES) from 72 additional animals. Support has also been obtained for WGS on an additional 200 animals during the budget period for this proposal. These efforts are supported by core TNPRC scientists in collaboration with researchers from other NPRCs and research institutions. These efforts are made in concert with the broader NPRC community through consortia activities, notably, the Nonhuman Primate Genetics and Genomics Working Group. Presently, the efforts of the genetic management program and MHC Genetic Typing Core are to continue to provide high-quality MHC characterization of the SPF breeding colony in support of HIV/AIDS research, to integrate MHC typing with other genetic and genomic data currently being characterized at the TNPRC, and to develop additional support structures for sharing this data with the research community. These efforts ensure the SPF rhesus macaque breeding colony is healthy, sustainable, and of maximal translational research value to the broader HIV/AIDS research community.

项目摘要/摘要-MHC遗传分型核心 Tulane国家灵长类动物研究中心的SPF恒河猴猕猴育种殖民地艾滋病研究 （TNPRC）是一项广泛的循证，综合计划的一部分，该计划纳入了健康方面的最佳实践 监视，饲养，预防医学以及行为和遗传管理。遗传的目标 管理计划是1）保持遗传多样性并最大程度地减少近交以支持可持续性 人群和2）特征是I类MHC等位基因以支持HIV/AIDS研究活动。为此， TNPRC基因组库收集和存储来自9000多种独特动物的材料，还有其他 产生的动物收集。殖民地的特征是祖先地理原生物和 使用PAN-NPRC SNP面板确认了Parentage。所有动物的MHC打字包括测试10级I 等位基因，它满足了大多数核心和会员科学家的需求，并在合作中进行了深入测序 威斯康星州国家灵长类动物研究中心的目标研究计划中心。超过以前的赠款 为164个关键育种者添加了整个基因组测序（WGS）的资金期，重点是这些雄性 育种菌落中最大的生殖产量，以及72的整个外显子组测序（WES） 其他动物。在预算期间，还获得了WGS对WGS的支持 该提议的期限。这些努力得到了核心TNPRC科学家的支持 来自其他NPRC和研究机构。这些努力是与更广泛的NPRC共同做出的 通过财团活动，特别是非人类灵长类动物遗传学和基因组学工作组的社区。 目前，遗传管理计划和MHC遗传打字核心的努力将继续 提供SPF育种菌落的高质量MHC表征，以支持HIV/AIDS研究，以 将MHC键入与目前在TNPRC中表征的其他遗传和基因组数据相结合，并将其整合到 开发与研究界共享此数据的其他支持结构。这些努力确保了 SPF恒河猕猴繁殖菌落是健康，可持续性和最大翻译研究价值的 向更广泛的艾滋病毒/艾滋病研究界。