Viral Testing Core

病毒检测核心

• 批准号: 10331080
• 负责人: Carolina Allers
• 金额: $ 22.85万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-09 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10331080
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Antigens; Biological Assay; Breeding; Cercopithecine Herpesvirus 1; Data; Detection; Development; Diagnostic Services; Future; Grant; Laboratories; Macaca; Maintenance; Microbiology; Mission; Pathogen detection; Performance; Primates; Research; Research Personnel; Research Support; Rivers; SIV; Sampling; Shipping; Simian Retroviruses; Site; Subgroup; Surveillance Program; Techniques; Testing; Time; United States National Institutes of Health; Validation; Validity of Results; Veterinary Medicine; Viral; Viral Physiology; Virus; animal colony; cost; diagnostic assay; interest; nonhuman primate; offspring; pathogen; performance tests; programs; quality assurance; screening; success; targeted agent; working group

Project Summary/Abstract - Viral Testing Core Continuous, accurate surveillance for agents of concern is an essential requirement for maintenance of a SPF breeding colony. In 2009 we transitioned from testing at off-site laboratories to performing all screening assays at the Tulane National Primate Research Center’s (TNPRC) Pathogen Detection and Quantification Core (PDQC). The PDQC is administratively located in the Division of Microbiology and was established at the TNPRC in 2004. The primary mission of the PDQC is to provide diagnostic services to the Division of Veterinary Medicine for establishing and maintaining SPF nonhuman primate colonies. A secondary mission is to provide research support to investigators. The development of onsite viral testing capabilities has resulted in reduced costs, eliminated shipping of samples, reduced turnaround time, and provided a platform for easy expansion for detection of additional agents. Quality assurance of the TNPRC SPF surveillance program is supported by the participation of the PDQC in proficiency panels testing from the Pathogen Detection Working Group (PDWG) of the NIH Nonhuman Primate Research Consortium (NHPRC) and the External Quality Assurance Program Oversight Laboratory (EQAPOL) Luminex program. This section of the application focuses on the function of the Viral Testing Core, techniques used for identification and detection of the target viruses, assay performance and validation, and current and future expansion of testing capabilities. The Aim of this core is to assure the SPF status of the colony through continued viral testing of existing breeding colony animals and all offspring. The SPF4 colony will be tested a minimum of twice each year for all four target viruses: Type D simian retrovirus (SRV), simian T-lymphotropic virus (STLV), simian immunodeficiency virus (SIV), and herpes B virus (BV) for SPF4. Characterization of the colony beyond the targeted agents supported by the U42 grant will continue through the development and use of additional assays for other pathogens of potential interest.

项目摘要/摘要 - 病毒检测核心 对受关注物质进行持续、准确的监测是维持 SPF 的基本要求 2009 年，我们从场外实验室测试转向进行所有筛选分析。 杜兰国家灵长类动物研究中心 (TNPRC) 病原体检测和定量核心 (PDQC) 行政上隶属于微生物学司，并在 TNPRC 设立。 2004 年。PDQC 的主要任务是为兽医部门提供诊断服务 用于建立和维持 SPF 非人类灵长类动物群体的次要任务是提供研究。 现场病毒检测能力的发展降低了成本， 消除了样品运输，缩短了周转时间，并提供了一个易于扩展的平台 TNPRC SPF 监测计划的质量保证得到了 TNPRC SPF 监测计划的支持。 PDQC 参与病原体检测工作组 (PDWG) 的能力小组测试 NIH 非人类灵长类动物研究联盟 (NHPRC) 和外部质量保证计划 监督实验室 (EQAPOL) Luminex 程序的这一部分重点介绍了 Luminex 程序的功能。 病毒检测核心，用于识别和检测目标病毒的技术、分析性能和 验证以及当前和未来测试能力的扩展。 该核心的目的是通过对现有育种的持续病毒测试来确保菌落的 SPF 状态 SPF4 群体的所有四个目标每年至少进行两次测试。 病毒：D型猴逆转录病毒（SRV）、猴T淋巴细胞病毒（STLV）、猴免疫缺陷病毒 (SIV) 和 B 型疱疹病毒 (BV) 的 SPF4 菌落特征超出了支持的目标药物。 U42 拨款将继续开发和使用针对其他病原体的其他检测方法 潜在的兴趣。