Role of Ankyrin-B in the Nervous System

锚蛋白-B 在神经系统中的作用

基本信息

批准号：
10330995
负责人：
Damaris N Lorenzo
金额：
$ 33.42万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-01-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10330995
关键词：
ANK2 gene ASD patient Adult Affect Anatomy Ankyrins Axon Axonal Transport Binding Biochemical Biological Assay Brain Brain Diseases Brain region Cell Adhesion Molecules Cell physiology Cells Complex Corpus Callosum Couples Cytoskeleton Data Defect Development Disease Etiology Failure Functional disorder Gene Delivery Glutamates Goals Grant Growth Human Image Impairment In Vitro Knock-out Knockout Mice Lead Length Maintenance Mediating Membrane Mental disorders Metabotropic Glutamate Receptors Microtubules Motor Mus Mutation Nervous system structure Neural Cell Adhesion Molecule L1 Neuraxis Neurodegenerative Disorders Neurodevelopmental Disability Neurologic Neurons Parkinson Disease Pathway Analysis Patients Phenotype Play Process Protein Isoforms Proteins Regulation Research Resolution Role Schizophrenia Semaphorin-3 Signal Transduction Somatosensory Cortex Spectrin Structure Synapses Synaptic Transmission Testing Variant Vertebral column Vesicle autism spectrum disorder axon growth axon guidance base cell motility de novo mutation density dynactin gene network in utero in vivo morphometry mouse model mutant nervous system disorder neuron development neuron loss neurotransmission novel postnatal period postsynaptic receptor synaptic function synaptic pruning synaptogenesis trafficking transmission process white matter

项目摘要

The establishment of functional neuronal networks in the developing and adult central nervous system (CNS) requires proper axonal specification, growth, branching, targeting, and synaptogenesis. Failure to appropriately interconnect brain regions during development or to refine those connections during maturation can lead to neurodevelopmental disabilities, such as autism, or to neurodegenerative and psychiatric disorders. De novo mutations in ANK2, which encodes ankyrin-B (AnkB), have been identified in autism spectrum disorder (ASD) patients, some of whom show aberrant axonal development. Neuronal loss of AnkB isoforms in mice results in absence of long axonal projections in the CNS and an overall reduction in axonal length, confirming that AnkB serves important roles in neuronal development in both humans and mice. AnkB has two major isoforms in the brain; ubiquitously expressed 220kDa (AnkB220) and neuron-specific 440kDa AnkB (AnkB440). We recently discovered that AnkB220 is motile and promotes microtubule-based axonal transport in cultured neurons to facilitate axonal growth. In contrast, AnkB440 interacts with cell adhesion molecules implicated in axon guidance and synaptogenesis. Neurons lacking AnkB440 have increased axon branching and synaptogenesis. We also found that AnkB is enriched at the postsynaptic density of glutamatergic synapses. The different phenotypes of the isoform-specific knockout in mice highlights the specialized functions of AnkB220 and AnkB440. Thus, there is a need to uncover the functional roles of neuronal AnkB and discern the cellular specialization of its AnkB220 and AnkB440 isoforms. Here, we will use novel mouse models lacking AnkB220 or AnkB440 in cortical neurons to unravel the precise cellular mechanisms underlying the neuronal development and connectivity deficits caused by the loss of these isoforms. Our research constitutes a novel effort to test our central hypothesis that AnkB coordinates neuronal structural and functional connectivity through the combined and specific roles of the AnkB220 and AnkB440 isoforms. To achieve our goals, we aim to: (1) Determine if AnkB220-driven axonal transport is required for the development and maintenance of long-range CNS axons in vivo; (2) Define molecular interactions required for AnkB440-mediated regulation of synaptic connections during brain development; and (3) Define the roles of AnkB in the postsynapse. Our studies will directly contribute to our understanding of the fundamental mechanisms of axonal growth and synaptogenesis, thereby informing the pathophysiology of ankyrin-related neurological and other brain disorders associated with deficits in white matter and synaptic connectivity.

发育中和成人中枢神经系统（CNS）中功能神经元网络的建立需要适当的轴突规格、生长、分支、定位和突触发生。未能适当在发育过程中互连大脑区域或在成熟过程中完善这些连接可能会导致神经发育障碍，例如自闭症，或神经退行性疾病和精神疾病。从头编码锚蛋白 B (AnkB) 的 ANK2 突变已在自闭症谱系障碍 (ASD) 中被发现患者，其中一些表现出异常的轴突发育。小鼠 AnkB 亚型的神经元丢失导致中枢神经系统中缺乏长轴突投射，轴突长度总体减少，证实 AnkB 在人类和小鼠的神经元发育中发挥重要作用。 AnkB 在大脑中有两种主要亚型；普遍表达的 220kDa (AnkB220) 和神经元特异性 440kDa AnkB (AnkB440)。我们最近发现 AnkB220 具有运动性并促进基于微管的培养神经元中的轴突运输以促进轴突生长。相反，AnkB440 与细胞粘附相互作用参与轴突引导和突触发生的分子。缺乏 AnkB440 的神经元轴突增加分支和突触发生。我们还发现 AnkB 在谷氨酸能突触后密度处富集。突触。小鼠体内异构体特异性敲除的不同表型凸显了特殊功能 AnkB220 和 AnkB440。因此，有必要揭示神经元 AnkB 的功能作用并辨别其 AnkB220 和 AnkB440 亚型的细胞特化。在这里，我们将使用皮质神经元中缺乏 AnkB220 或 AnkB440 的新型小鼠模型来揭示精确的神经元发育和连接缺陷背后的细胞机制是由这些缺失引起的同工型。我们的研究构成了一项新颖的努力来检验我们的中心假设：AnkB 协调神经元通过 AnkB220 和 AnkB440 的组合和特定作用实现结构和功能连接同工型。为了实现我们的目标，我们的目标是：（1）确定 AnkB220 驱动的轴突运输是否需要体内长程中枢神经系统轴突的发育和维持； (2) 定义所需的分子相互作用 AnkB440 介导的大脑发育过程中突触连接的调节； (3) 定义角色 AnkB 位于突触后。我们的研究将直接有助于我们对基本原理的理解轴突生长和突触发生的机制，从而了解锚蛋白相关的病理生理学与白质和突触连接缺陷相关的神经系统和其他大脑疾病。