INTERMEDIATE FILAMENT--CELL SURFACE INTERACTIONS

中间丝--细胞表面相互作用

• 批准号: 2178532
• 负责人: ROBERT D GOLDMAN
• 金额: $ 25.06万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2178532
• 关键词: active sites; cell cell interaction; cell membrane; cytoskeleton; intermediate filaments; microinjections; molecular cloning; phosphorylation; protein kinase; protein sequence; protein structure function; site directed mutagenesis; tissue /cell culture

DESCRIPTION (Adapted from applicant's abstract): The work described in this project is aimed at determining the structure, properties, and functions of intermediate filaments (IF) in mammalian cells. Many of the experiments take advantage of recently discovered properties of IF showing that they are dynamic cytoskeletal proteins. Dr. Goldman proposes to use specific peptides designed to disrupt IF assemblies in vivo using microinjection methods and to determine the physiological changes which accompany their disassembly. He also proposes to study the mechanisms by which phosphorylation modifies IF organization in cells using a variety of cellular and molecular approaches, including microscopic, biochemical, and immunological techniques. Another major goal is to determine the function of one of the major IF associated proteins, IFAP300. This protein appears to be involved in regulating the distribution and supramolecular organization of IF networks in cells via its crossbridging function. IFAP300 also appears to be involved in interactions with other cytoskeletal systems such as microtubules. In order to further understanding of the function of this protein, it is being cloned and sequenced using standard methods.

描述（改编自申请人的摘要）：中描述的工作 该项目旨在确定结构、属性和 哺乳动物细胞中中间丝（IF）的功能。许多 该实验利用了最近发现的 IF 特性 显示它们是动态细胞骨架蛋白。戈德曼博士 建议使用旨在破坏 IF 组装的特定肽 体内使用显微注射方法并测定生理学 伴随拆卸而发生的变化。他还建议学习 磷酸化改变 IF 组织的机制 使用多种细胞和分子方法对细胞进行研究，包括 显微镜、生化和免疫学技术。另一个专业 目标是确定相关主要 IF 之一的功能 蛋白质，IFAP300。这种蛋白质似乎参与调节 细胞内 IF 网络的分布和超分子组织 其跨桥功能。 IFAP300 似乎也参与了 与其他细胞骨架系统（例如微管）的相互作用。在 为了进一步了解该蛋白的功能， 使用标准方法进行克隆和测序。