Berghia BRAIN project

Berghia BRAIN 项目

基本信息

批准号：
10302199
负责人：
Paul S Katz
金额：
$ 100万
依托单位：
UNIVERSITY OF MASSACHUSETTS AMHERST
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10302199
关键词：
3-Dimensional Accounting Address Adolescent Adult Affect Algorithms Anatomy Animals Atlases Awareness Behavior Behavior Control Behavioral Body part Brain Calcium Cell Lineage Code Complex Decision Making Development Disease Dyes Electrons Enteric Nervous System Exhibits Ganglia Gene Expression Genetic Genomics Goals Health Human Image Immune system Immunohistochemistry In Situ Hybridization Insecta Institution Intelligence Knowledge Laboratories Mammals Mechanics Memory Microscopy Modeling Modernization Nerve Net Neuraxis Neurons Neurosciences Neurosecretory Systems Octopus Odors Olfactory Pathways Optics Organism Organizational Models Pathologic Pathway Analysis Peripheral Phylogenetic Analysis Physiological Process Production Protocols documentation Reaction Research Role Smell Perception Statistical Models Structure Synapses System Tactile Techniques Testing Vertebrates Vision Work appendage arm behavioral response biophysical model body system connectome experimental study fluorescence imaging holistic approach innovation insight multisensory network models neural network neurogenesis novel nudibranch olfactory stimulus reconstruction relating to nervous system response sensor sensory input sensory system tool transcriptome sequencing visual stimulus voltage water flow

项目摘要

PROJECT SUMMARY / ABSTRACT - Overall The Berghia Brain Project is a multi-institution collaborative project to examine fundamental issues about how brain circuits interact with distributed networks. There is a growing awareness of the importance of peripheral networks to human health, but it is difficult to study how such distributed networks interact at a cellular level with the brain in mammals. This project focuses on the nudibranch mollusc, Berghia stephanieae because it provides a unique opportunity to challenge fundamental concepts in neuroscience. The project examines the dynamics, network structure, algorithms, and neural implementation of behaviors related to mechanosensation, olfaction, and vision. The project addresses the critical need for phylogenetic diversity in neuroscience by developing genetic tools and protocols to make Berghia an accessible laboratory species for neuroscience. Berghia has a small brain with fewer than 5000 neurons in the adult and one tenth that number in juveniles. Understanding the impact of neurogenesis on functioning networks has implications for human memory circuits, which also gain neurons. In addition, Berghia has over 9000 neurons in each of its olfactory (rhinophore) ganglia and untold numbers of neurons that form a sub-epidermal nerve net on the animal’s body. The project will create a multi- feature, multi-scale atlas of neurons, which will feature connectomes of the adult brain and the whole body of a juvenile obtained from serial reconstruction of electron micrographs. These connectomes will be aligned with fluorescence images of neuronal gene expression and immunohistochemistry. The project will test solutions to automatically segment images and rapidly create wiring diagrams. Neurons will be further identified through cell- lineage tracing to determine the origins of identified neurons and classes of other neurons. Analysis of single neuron RNA sequencing will provide new insights into circuitry and neural identity. Expansion microscopy will allow a rich accounting of peripheral neurons. The activity of neurons in the peripheral net and the brain will be optically recorded with voltage- and calcium-sensitive dyes and genetically-encoded sensors to image information flow in response to mechanical, olfactory, and visual stimuli. The project will test standard hypotheses related to how these systems work in a non-standard organism with the goal of determining the generality of canonical concepts such as somatotopic mapping of the body, glomerular organization of olfactory systems, and modular organization of vision.

项目概要/摘要 - 总体 Berghia Brain 项目是一个多机构合作项目，旨在研究有关如何人们越来越意识到外围设备的重要性。网络对人类健康的影响，但很难研究这种分布式网络如何在细胞水平上与该项目重点关注裸鳃类软体动物 Berghia stephanieae，因为它提供了挑战神经科学基本概念的独特机会该项目研究了动态，与机械感觉、嗅觉、该项目通过开发解决了神经科学系统发育多样性的迫切需求。使 Berghia 成为神经科学可用的实验室物种的遗传工具和协议。成年人的大脑很小，神经元数量不到 5000 个，而青少年的神经元数量只有十分之一。神经发生对功能网络的影响对人类记忆回路有影响，这也获得了此外，Berghia 的每个嗅觉（犀牛）神经节和无数的神经元都有超过 9000 个神经元。该项目将在动物的身体上形成表皮下神经网络的神经元数量。特征，神经元的多尺度图谱，其中包含成人大脑和整个身体的连接组从电子显微照片的连续重建中获得的幼体将与这些连接组对齐。该项目将测试神经元基因表达和免疫组织化学的荧光图像。自动分割图像并快速创建接线图，通过细胞进一步识别神经元。谱系追踪以确定已识别神经元的起源和其他神经元的类别分析。神经元 RNA 测序将为电路和神经特性提供新的见解。允许对周围神经元进行丰富的统计周围网络和大脑中神经元的活动将被使用电压和钙敏感染料和基因编码传感器进行光学记录以成像响应机械、嗅觉和视觉刺激的信息流该项目将测试标准假设。与这些系统如何在非标准有机体中工作相关，目的是确定这些系统的普遍性规范概念，例如身体的体位图、嗅觉系统的肾小球组织，以及视觉的模块化组织。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Importance of Being Correlated: Implications of Dependence in Joint Spectral Inference across Multiple Networks

相关性的重要性：跨多个网络的联合谱推理中的依赖性的含义

DOI：
发表时间：
2020-08-01
期刊：
Journal of machine learning research : JMLR
影响因子：
0
作者：
Konstantinos Pantazis;A. Athreya;Jesús Arroyo;W. Frost;E. Hill;V. Lyzinski
通讯作者：
V. Lyzinski

State-dependent, visually guided behaviors in the nudibranch Berghia stephanieae.

裸鳃类动物 Berghia stephanieae 的状态依赖性、视觉引导行为。

DOI：
发表时间：
2023-09-15
期刊：
影响因子：
0
作者：
Quinlan, Phoenix D;Katz, Paul S
通讯作者：
Katz, Paul S

Cephalopod vision: How to build a better eye.

头足类动物视力：如何培养更好的眼睛。

DOI：
发表时间：
2023-01-09
期刊：
影响因子：
0
作者：
Katz, Paul S;Lyons, Deirdre C
通讯作者：
Lyons, Deirdre C

Movement and storage of nematocysts across development in the nudibranch Berghia stephanieae (Valdés, 2005).

裸鳃类Berghia stephanieae 线虫囊在发育过程中的运动和储存（Valdés，2005）。

DOI：
发表时间：
2022-04-18
期刊：
影响因子：
0
作者：
Goodheart, Jessica A;Barone, Vanessa;Lyons, Deirdre C
通讯作者：
Lyons, Deirdre C

A chromosome-level genome for the nudibranch gastropod Berghia stephanieae helps parse clade-specific gene expression in novel and conserved phenotypes.

裸鳃类腹足动物 Berghia stephanieae 的染色体水平基因组有助于解析新颖和保守表型中进化枝特异性基因的表达。

DOI：
发表时间：
2023-11-16
期刊：
影响因子：
0
作者：
Goodheart, Jessica A;Rio, Robin A;Taraporevala, Neville F;Fiorenza, Rose A;Barnes, Seth R;Morrill, Kevin;Jacob, Mark Allan C;Whitesel, Carl;Masterson, Park;Batzel, Grant O;Johnston, Hereroa T;Ramirez, M Desmond;Katz, Paul S;Lyons, Deirdre C
通讯作者：
Lyons, Deirdre C