Single cell glycomics: mapping the surface glycan signature of cells

单细胞糖组学：绘制细胞表面聚糖特征

• 批准号: DP200102004
• 负责人: Prof Yuling Wang
• 金额: $ 28.86万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-05-18 至 2023-05-17
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200102004
• 关键词: Single; cell; glycomics; mapping; surface

This project aims to develop a platform technology for multiplexed glycan mapping of the surface of a single cell to address challenges of functional glycomics by utilising a conceptually new approach. By combining newly designed plasmonic nanoparticles with surface-enhanced Raman scattering tags and multiple specific carbohydrate-recognising lectins, this project expects to produce a generic technology that is capable of non-destructive barcoding of the surface glycan signature of single cells in their native state and in response to metabolic perturbations. Expected project outcomes include advancing knowledge in nanobiotechnology, glycobiology and cell biology by being able to easily monitor changes to the surface of single cells.

该项目旨在开发一种用于单细胞表面多重聚糖图谱的平台技术，以利用概念性新方法应对功能糖组学的挑战。通过将新设计的等离子体纳米粒子与表面增强拉曼散射标签和多种特定碳水化合物识别凝集素相结合，该项目期望产生一种通用技术，能够对处于天然状态的单细胞表面聚糖特征进行无损条形码编码，并响应代谢扰动。预期的项目成果包括通过能够轻松监测单细胞表面的变化来推进纳米生物技术、糖生物学和细胞生物学的知识。