TAS::75 0849::TAS INCREMENTAL FUNDING

塔斯马尼亚州::75 0849::塔斯马尼亚州增量资金

• 批准号: 10273219
• 负责人: LEONARD FREEDMAN
• 金额: $ 1.95万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-31 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10273219
• 关键词: Adherence; Aliquot; Animals; Biological; Blood; Blood specimen; Breast Cancer Risk Factor; CCR; Cancerous; Cell Culture Techniques; Cell Proliferation; Cells; Cervical; Clinical; Clinical Trials; Collection; Colon Carcinoma; Communication; DNA; Data Analyses; Databases; Diet; Differentiation and Growth; Flavonoids; Food; Freezing; Funding; Future; Generations; Genistein; Human; Human Resources; Imaging technology; In Vitro; Indole-3-Carbinol; Inflammation; Inflammatory; Joints; Laboratories; Leukocytes; Liquid substance; Maintenance; Measurement; Messenger RNA; Monitor; National Surgical Adjuvant Breast and Bowel Project; Natural regeneration; Nitrogen; Normal Cell; Physiological; Plasma; Process; Proteomics; Research; Resveratrol; Retrieval; Risk Behaviors; Rodent Model; Role; Sampling; Scientist; Services; Shipping; Specific qualifier value; Specimen; Structure of nail of toe; Sulfur; Technology; Testing; Tissue Sample; Tumor Stem Cells; Vial device; Visit; Woman; bean; biobank; men; neoplastic cell; repository; statistical center; stem cell differentiation; stem cells; transcriptomics; tumor; tumor behavior

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.

这项支持是与 CCR（Farrar 实验室）联合研究计划的一部分，旨在检查各种食物成分作为肿瘤干细胞行为调节剂的作用，并最终对肿瘤再生产生影响。这些体外研究使用细胞培养技术来评估其影响。食品成分的生理相关浓度对癌性和非癌性干细胞的分化进行了一些生物学测量，以评估吲哚 3-甲醇、染料木黄酮、白藜芦醇、烯丙基硫、这项支持是与 CCR（Timothy Veenstra 博士和 Thomas Turbyville 实验室）联合研究计划的一部分，旨在检查使用蛋白质组学技术来评估各种食物成分作为乳腺癌风险调节剂的作用。使用培养细胞的体外研究和动物研究将用于评估食物成分，最初的研究将集中于金雀异黄素作为培养物中正常细胞和肿瘤细胞的调节剂。 3-甲醇、白藜芦醇对蛋白质组学变化以及这些变化与细胞增殖的关系是与 CCR（科尔伯恩实验室）联合研究计划的一部分，旨在检查各种食物成分作为炎症调节剂的作用以及最终对结肠的影响。结合成像技术的动物研究被用来监测饮食的影响，以评估吲哚 3-甲醇、染料木黄酮、白藜芦醇的影响。 Fisher Bio Services 从贡献实验室接收临床样本，将指定样本等分以构建参考集，将每个样本的标识符记录到数据库中，保存所有这些样本并将参考集发送到数据库。只要临床试验期间收集的血液和宫颈细胞样本保存在冰箱中，这将是一个持续的项目。其他血液和宫颈样本储存在液氮冰箱中，这项支持是为了补偿 Frederick 完成人类和啮齿动物模型样本的转录组 (mRNA) 分析，并将提供给进行该研究的人员。所提供的大多数样本都需要进行初步提取，这些分析数据以电子方式提供给我们进行独立分析，其中包括来自 SELECT 临床试验的血液、脚趾甲、白细胞和血浆。正在进行的活动包括处理 2500 名过渡访问男性的血液、储存自 2000 年研究启动以来的所有血液、每周生成 BSI 文件、与 SELECT 统计中心人员持续沟通、从依从性小组运送样本。研究中，STAR 的 NSABP 存储库提取了大约 400 个样本的 DNA，包括样本的持续储存以及长期储存血液、白细胞和血浆的冰箱的维护。 STAR 临床试验有 19,000 名女性参与，任务包括定期检查标本和冰箱、采集标本、记录存储以及与 NSABP 互动。