MOLECULAR EVOLUTIONARY GENETICS OF HAWAIIAN DROSOPHILA CHORION

夏威夷果蝇绒毛膜的分子进化遗传学

• 批准号: 3734287
• 负责人: J C MARTINEZ-CRUZADO
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO MAYAGUEZ
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734287
• 关键词: Drosophilidae; Hawaii; alcohol dehydrogenase; animal population genetics; biochemical evolution; chorioallantoic membrane; complementary DNA; developmental genetics; field study; gene expression; genetic polymorphism; genetic regulation; genetic regulatory element; genetic transcription; in situ hybridization; molecular cloning; molecular genetics; nucleic acid sequence; polymerase chain reaction; species difference; transposon /insertion element

In recent years, it has been firmly established that transcriptional regulation plays a fundamental role in the developmental and physiological regulation of eucaryotic systems. In fact, it may be that genotypic variation governing phenotypic variation between species exists mostly at the transcriptional rather than at the structural level. We will study the mode and the frequency of transcriptional regulation evolution using as a model system the rich reservoir of eggshell phenotypic variants established in short time periods in the evolution of Hawaiian Drosophila. Specifically, we will gauge the extent of natural transcriptional variation by means of the characterization of the transcriptional regulation of various late minor chorion genes in several species exhibiting diverging chorion surface phenotypes. We will then identify a transcription variating gene whose developmental expression can be reproduced in a D. melanogaster background. This will lead to future experiments that will identify cis-acting regulatory elements as well as their mode of function and evolution. The fundamental approach is to use the natural transcription variants of the gene tb help select, by sequence analysis, candidate regulatory DNA elements. In vivo assays of mutagenized versions of these candidates will identify the elements responsible for the observed natural variation as well as their function in subsequent experiments. In addition, whole mount in situ hybridization of late minor chorion cDNAs to developing follicles will expand our knowledge on the number and distribution of their messengers, and will suggest functional significances for the natural transcription variants.

近年来，它已经牢固地确定了该转录 监管在发展中起着基本作用 桉树系统的生理调节。 实际上，可能是 存在于物种之间表型变异的基因型变异 主要是在转录，而不是在结构层面上。 我们将研究转录调节的模式和频率 进化用作模型系统蛋壳的丰富储层 表型变体在短时间内建立 夏威夷果蝇。 具体来说，我们将衡量自然的程度 通过表征的转录变化 几个晚期辅助基因的转录调节 表现出不同绒毛膜表面表型的物种。 然后我们会 确定转录变异基因的发育表达 可以在D. melanogaster背景中复制。 这将导致 未来的实验将确定顺式作用调节元素为 以及它们的功能和进化方式。 基本方法 是使用基因结核的自然转录变体有助于选择， 通过序列分析，候选调节性DNA元素。 体内测定 这些候选人的诱变版本将确定要素 负责 观察到的自然变化以及它们在随后的功能 实验。 此外，晚期小型绒毛膜的原位杂交整体 开发卵泡的cDNA将扩大我们对数量和数量的知识 分配其使者，并建议功能 自然转录变体的重要性。