BIOMARKERS FOR OCCUPATIONAL SOLVENT EXPOSURE

职业溶剂暴露的生物标志物

• 批准号: 2155407
• 负责人: Grace LeMasters
• 金额: $ 24.33万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2155407
• 关键词: DNA damage; air pollution; air sampling /monitoring; biomarker; breath tests; chromatin; cytotoxicity; environmental contamination; environmental toxicology; ethane; gas chromatography mass spectrometry; genetic crossing over; human subject; industry; ketones; longitudinal human study; male; micronucleus; mutagens; occupational hazard; occupational health /safety; semen; sperm; sperm motility; toluene

Although millions of individuals are exposed to solvent mixtures at work, home and through the air, water, and food contamination, relatively few human studies have addressed the reproductive and genetic consequences of exposure. This study addresses these concerns and represents an optimum design as it is a prospective cohort study with a repeated measures design. Biological samples are collected pre- and post-exposure over three periods separated by fifteen week intervals. The study hypothesis is that duration and intensity of exposure to solvents at levels below the threshold limit values as measured with industrial hygiene sampling data and levels as measured in breath samples will be associated with an increase in adverse mutagenic and spermatogenic alterations. Trends over time will be noted between the baseline (pre-exposure and the two post-exposure biological assays. The specific aims of the study are to 1) test a battery of reproductive and mutagenic assays as early indicators of solvent exposures and 2) to determine the correlation and predictive ability between external exposure measures and internal dose measures and to compare each of these biologic effect markers. The mixed solvent exposure includes 1,1,1- trichloro-ethane, methyl ethyl ketone, xylene and toluene. Twenty-five exposed and 5 control subjects will be followed for a period of one year. Pre exposure mutagen assays and semen analysis are obtained as a baseline measure and then repeated post exposures at two intervals. Breath samples are collected at end of shift on the same day as the other biological samples. In addition, a total of eighteen industrial hygiene measurements divided between sample intervals are collected on each individual to obtain a composite index of a worker's cumulative exposure as well as exposure during each fifteen week period. This study represents an unique opportunity in that a Memorandum of Understanding with the United States Air Force is already in place in support of the study and has the approval of the Air Force Surgeon General's Office. In addition, a pilot study has been completed. The study is being conducted at a military installation, Hill Air Force Base with the voluntary cooperation of military and civilian employees and the full support of the union.

尽管数以百万计的人在工作中暴露于溶剂混合物中，但 家，通过空气，水和食物污染，相对较少 人类研究已经解决了生殖和遗传后果 接触。 这项研究解决了这些问题，并代表了最佳 设计是一项具有重复措施设计的前瞻性队列研究。 在三个时期内收集生物样品 隔开15周间隔。 研究假设是持续时间 在低于阈值限制的水​​平上暴露于溶剂的强度和强度 用工业卫生采样数据和水平测量的值 在呼吸样品中测量的情况与不利的增加有关 诱变和精子发生变化。 随着时间的流逝，趋势将被注意 在基线之间（暴露前和两种暴露后生物学 测定。 该研究的具体目的是1）测试一电池 繁殖和诱变测定作为溶剂暴露的早期指标 2）确定外部的相关性和预测能力 暴露措施和内部剂量措施并比较这些 生物效应标记。 混合溶剂暴露包括1,1,1-- 三氯 - 乙烷，甲基乙烯酮，二甲苯和甲苯。 将遵循25个暴露和5名对照对象 一年。 在暴露前诱变分析和精液分析作为 基线度量，然后以两个间隔重复进行后接触。 与另一个 生物样品。 此外，总共十八个工业卫生 在每个样品间隔之间划分的测量值 个人获得工人累积暴露的综合指数作为 以及在每个15周期间的暴露。 这项研究代表了一个独特的机会 与美国空军的了解已经存在 对研究的支持，并获得了空军外科医生的批准 将军办公室。 此外，一项试点研究已经完成。 这 在希尔空军基地的军事装置上进行了研究 随着军事和民用雇员的自愿合作， 联盟的全部支持。