ShEEP Request for ImageXpress Micro Confocal High Content Screening System

ShEEP 请求 ImageXpress Micro 共焦高内涵筛选系统

• 批准号: 10177122
• 负责人: James Michael Fleckenstein
• 金额: --
• 依托单位: ST. LOUIS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177122
• 关键词: 3-Dimensional; 4D Imaging; Adopted; Basic Science; Biological Models; Blood; California; Cancer Biology; Cardiology; Cells; Chemotherapy-Oncologic Procedure; Communicable Diseases; Complex; Computer software; Confocal Microscopy; Devices; Disease; Disease Management; Equipment; Fluorescence; Four-dimensional; Funding; Goals; Health; Healthcare Systems; Image; Immersion; In Vitro; Individual; Investigation; Laboratories; Lasers; Light; Manufacturer Name; Microscope; Molecular; Nature; Nephrology; Noise; Optics; Organ; Organoids; Pathogenesis; Patients; Penetration; Phase; Population Heterogeneity; Predisposition; Prevention strategy; Principal Investigator; Program Evaluation; Reporter; Research; Research Personnel; Resolution; Rheumatology; Saints; Sampling; Scientist; Signal Transduction; Source; Speed; System; Testing; Thick; Time; Tissues; Translations; Universities; Veterans; Washington; Water; Whole Organism; adult stem cell; cellular imaging; chemotherapeutic agent; computerized data processing; confocal imaging; contrast imaging; design; drug testing; high resolution imaging; high throughput screening; human tissue; image processing; imaging capabilities; imaging system; instrument; instrumentation; live cell imaging; medical schools; microbial; novel therapeutics; novel vaccines; personalized approach; programs; screening; stem cells; therapy development; tool; welfare

Project Summary/Abstract The goal of this ORD Shared Equipment Evaluation Program (ShEEP) proposal from the Saint Louis VA Healthcare System is to obtain a high content confocal microscopy imaging system capable of obtaining and processing high resolution images of multiple samples, including live cells. The system will be shared by multiple VA-funded investigators and their colleagues at two affiliated universities Saint Louis University School of Medicine and Washington University School of Medicine. Both affiliates have substantial research enterprises connected to the Saint Louis VA. The instrument is designed to support and enhance the research endeavors of a large and diverse group of VA-funded scientists. The instrumentation requested, is a Molecular Devices ImageXpress Micro Confocal High-Content Imaging System and accompanying analysis software that includes multiple application modules pre- loaded onto an analysis workstation designed to support the imaging and data processing needs for a wide variety of investigations. The proposed system is configured for spinning disc laser confocal microscopy to accommodate rapid imaging at multiple fluorescence wavelengths, water immersion objectives that permit increased depth of penetration for thicker samples at reduced exposure times, environmental control for imaging of live samples and phase contrast for imaging of unstained cells. In addition to the need for high content imaging and analysis, a common theme among the investigators supporting this proposal is the need to image and analyze organoids. These adult stem cell-derived clusters of cells recapitulate many of the features from the tissue from which they were obtained and therefore have now been adopted in multiple lines of investigation from personalized cancer chemotherapy to microbial pathogenesis studies. Multiple projects in this proposal relating cancer biology, rheumatology, nephrology, cardiology and infectious diseases are designed around the imaging of organoids as a platform for basic discovery and translation to therapies that would benefit our patients at the VA.

项目概要/摘要 圣路易斯 ORD 共享设备评估计划 (ShEEP) 提案的目标 VA 医疗保健系统旨在获得高内涵共焦显微镜成像系统，能够 获取和处理多个样本（包括活细胞）的高分辨率图像。系统 将由 VA 资助的多名研究人员及其两所附属大学的同事共享 圣路易斯大学医学院和华盛顿大学医学院。两家附属公司 拥有与圣路易斯弗吉尼亚州相关的大量研究企业。该仪器的设计目的是 支持和加强由 VA 资助的大量多元化科学家的研究工作。 所需的仪器是 Molecular Devices ImageXpress Micro Confocal 高内涵 成像系统和附带的分析软件，包括多个预置应用模块 加载到分析工作站上，旨在支持成像和数据处理需求 各种各样的调查。所提出的系统配置用于旋转圆盘激光共焦 显微镜可适应多种荧光波长的快速成像、水浸 允许在减少暴露时间的情况下增加较厚样品的穿透深度的物镜， 用于活样品成像的环境控制和用于未染色细胞成像的相差。 除了对高内容成像和分析的需求之外， 研究人员支持这一提议的理由是需要对类器官进行成像和分析。这些成年茎 细胞衍生的细胞簇概括了它们所来自的组织的许多特征 已获得，因此现已在个性化的多方面调查中采用 癌症化疗到微生物发病机制研究。本提案中的多个项目涉及 癌症生物学、风湿病学、肾病学、心脏病学和传染病学都是围绕 类器官成像作为基础发现和转化为有益疗法的平台 我们在退伍军人管理局的病人。