Olfactory neuromodulation of visual circuits and behavior

视觉回路和行为的嗅觉神经调节

基本信息

批准号：
10177297
负责人：
Mark Arthur Frye
金额：
$ 38.05万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10177297
关键词：
Affect Aging Animals Ballistics Basic Science Behavior Behavioral Biogenic Amines Biological Models Brain Cells Chemicals Cognitive Complex Couples Degenerative Disorder Detection Discrimination Disease Drosophila genus Food Functional disorder Ganglia Genetic Models Genetic Techniques Goals Home Human Image Individual Knowledge Lead Link Locomotion Logic Maps Mission Modality Modeling Molecular Motion Motor Movement National Institute of Neurological Disorders and Stroke Nervous system structure Neurons Norepinephrine Octopamine Odors Olfactory Pathways Optic Lobe Optics Output Pathology Pathway interactions Pattern Perception Population Process Property Reflex action Research Resolution Saccades Sensory Signal Pathway Signal Transduction Smell Perception Smooth Pursuit Source Spatial Distribution Stimulus Stroke Synapses System Testing Transcript Traumatic injury United States Vision Visual Writing behavioral response brain health chemical function cognitive function detector disability falls fly functional disability improved in vivo in vivo calcium imaging multimodality multisensory neurogenetics neuronal circuitry neuroregulation nonhuman primate octopamine receptor optogenetics public health relevance receptive field relating to nervous system response sensor sensory input two-photon virtual reality virtual reality system visual control visual feedback visual processing

项目摘要

Project summary A significant percentage of people in the US suffer from disabilities resulting from traumatic injury, stroke, or degenerative disease which result in enigmatic deficits in cognitive function. Therefore, an understanding of the fundamental properties of neuronal circuits for complex brain function, and how these circuits are modulated by biogenic amines, will sharpen our understanding of the normal brain, thereby highlighting pathology and facilitating treatments. A major function of the brain is to integrate information across sensory modalities to enable sharp and robust perception. The cell-circuit mechanism for how different sensory modalities interact is not well understood. This project will capitalize on the significant experimental advantages of the fruit fly Drosophila to explore the molecular logic and neural connections that produce an elementary form of multisensory integration perception. The fly displays robust multisensory perception, integrating olfactory signals with visual processing to enhance perceptual abilities. Furthermore, sensory circuits have been shown to be under robust neuromodulatory control by biogenic amines. Similar processes have been localized to sub- cortical and cortical pathways in humans and non-human primates. The fly has a numerically compact nervous system, with which highly advanced genetic techniques can be used to identify, manipulate, and record activity from individual neurons and neurosecretory cells, as well as their upstream and downstream synaptic partners and molecular components. The PI hypothesizes that the fly brain couples olfactory sensory detection to visual processing through neuromodulatory cells that carry the chemical equivalent of norepinephrine. The PI will perform two-photon Ca2+ imaging to ‘read’ activity from live flies in response to stimuli the PI has discovered elicit robust multi-modal integration behavior within a virtual reality system. The PI will use optogenetics imaging to ‘write’ signals into these circuit pathways to assess input-output functions from an intact behaving fly.

项目概要在美国，很大一部分人因创伤而患有残疾损伤、中风或退行性疾病会导致认知功能出现神秘缺陷。因此，了解复杂大脑神经回路的基本特性功能，以及这些电路如何被生物胺调节，将提高我们的了解正常大脑，从而突出病理学并促进治疗。大脑的一个主要功能是整合各种感觉模式的信息，以实现不同感觉方式的细胞回路机制。这个项目将利用重要的实验。果蝇的优势探索分子逻辑和神经联系产生一种基本形式的多感官整合感知。多感官知觉，将嗅觉信号与视觉处理相结合，以增强此外，感知能力已被证明不够强大。生物胺的神经调节控制已被定位于亚- 人类和非人类灵长类动物的皮质和皮质通路苍蝇具有数字。紧凑的神经系统，可以使用高度先进的遗传技术识别、操纵和记录单个神经元和神经分泌细胞的活动，如以及它们的上游和下游突触伙伴和分子成分。果蝇大脑通过以下方式将嗅觉检测与视觉处理结合起来携带去甲肾上腺素化学当量的神经调节细胞将发挥作用。双光子 Ca2+ 成像可“读取”活果蝇响应刺激 PI 的活动发现在虚拟现实系统中引发强大的多模式集成行为。将使用光遗传学成像将信号“写入”这些电路通路以评估输入输出来自完整行为的苍蝇的功能。