Flow cytometer Cell Sorter for Biosafety Level-3 Facility

生物安全三级设施流式细胞仪细胞分选仪

• 批准号: 10177283
• 负责人: ANKITA GARG
• 金额: $ 54.7万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177283
• 关键词: 2019-nCoV; Address; Antigen-Antibody Complex; Area; Biological Assay; Biomedical Research; Bordetella; Brucella melitensis; Burkholderia mallei; Burkholderia pseudomallei; Cells; Communicable Diseases; Containment; Diagnostic; Ebola; Funding; Goals; HIV; Immune response; Immunity; International; Investigation; Laboratories; Maintenance; Mycobacterium tuberculosis; Organism; Pathogenesis; Pathogenicity; Pathology; Preclinical Testing; Process; Research; Research Personnel; Sampling; Sorting - Cell Movement; Speed; Therapeutic; United States Department of Agriculture; United States National Institutes of Health; Universities; Viral; biosafety level 3 facility; co-infection; disease transmission; experimental study; hemorrhagic fever virus; influenzavirus; instrument; novel vaccines; pathogen; population health; programs; respiratory; respiratory pathogen; transmission process; vaccine development; vaccine efficacy; vaccine evaluation

PROJECT SUMMARY/ABSTRACT Investigators at UGA have established national and international recognition for their research on high- containment pathogens of global importance. UGA investigators are working towards understanding pathogenesis and developing newer vaccines, therapeutics and diagnostics to confer sustained immunity to re- challenge with homologous or heterologous pathogen strains. However, to advance these programs, multiparametric flow cytometer with high speed sorting is critical to formulate assays which assess vaccine efficacy, disease transmission, host response, and pre-clinical testing of vaccine/therapeutics/diagnostic candidate(s). This capability is currently lacking at the University of Georgia. The major users for the proposed flow cytometer sorter comprise NIH-funded researchers from three Departments at UGA; Infectious Diseases, Population Health and Pathology. Additional users are investigators of United States Department of Agriculture. Currently, multiple flow cytometers and sorters are available at UGA, and three are equipped with Biosafety Cabinets for sorting infectious cells. However, none of the existing cytometers or sorters can be used for un-fixed/live samples that are processed in high-containment / Biosafety Level-3 (BSL3) or BSL3-Ag laboratories. Acquisition of the proposed flow cytometer-sorter will provide new capabilities that will allow investigators to address complex immunological research problems in the area of infectious diseases. Specific areas of investigation that will directly benefit by obtaining the proposed instrument are: 1) immune response and vaccine development for Brucella melitensis, Mycobacterium tuberculosis, Bordetella spp, influenza virus, SARS-CoV-2 and several others, 2) immune response to Human Immunodeficiency Virus (HIV) – Mycobacterium tuberculosis co-infection, 3) understanding of the transmission of respiratory pathogens, 4) host range and pathogenesis of respiratory viral and bacterial co-infection, 5) pathogenesis and immune response to Burkholderia mallei and Burkholderia pseudomallei and hemorrhagic fever viruses Ebola, Lassa and SARS-CoV-2. The lack of a cytometer-sorter in a high-containment facility has hindered UGA researchers ability to perform critical experiments with highly pathogenic organisms. The instrument will therefore facilitate the realization of research goals and maintenance of NIH support for biomedical research.

项目概要/摘要 佐治亚大学的研究人员的高水平研究已获得国内和国际认可。 佐治亚大学研究人员正在努力了解具有全球重要性的遏制病原体。 开发新的疫苗、治疗方法和诊断方法，以赋予持续的免疫力 然而，为了推进这些计划， 具有高速分选功能的多参数流式细胞仪对于制定评估疫苗的测定至关重要 疫苗/治疗/诊断的功效、疾病传播、宿主反应和临床前测试 乔治亚大学目前缺乏这种能力。 拟议的主要用户。 流式细胞仪分选机由美国国立卫生研究院 (NIH) 资助的佐治亚大学传染病研究所的研究人员组成； 其他用户是美国卫生部的调查人员。 目前，佐治亚大学拥有多台流式细胞仪和分选仪，其中三台配备了流式细胞仪和分选仪。 用于分选传染性细胞的生物安全柜然而，现有的细胞仪或分选机都不能使用。 适用于在高防护/生物安全 3 级 (BSL3) 或 BSL3-Ag 中处理的未固定/活样品 收购拟议的流式细胞仪分选仪将提供新的功能，使之能够实现。 研究人员解决特定传染病领域的复杂免疫学研究问题。 通过获得拟议的工具将直接受益的研究领域是：1）免疫反应 以及布鲁氏菌、结核杆菌、博德特氏菌、流感病毒、 SARS-CoV-2 和其他几种，2) 对人类免疫缺陷病毒 (HIV) 的免疫反应 – 结核分枝杆菌合并感染，3) 了解呼吸道病原体的传播，4) 呼吸道病毒和细菌共同感染的宿主范围和发病机制，5）发病机制和免疫 对鼻疽伯克霍尔德杆菌和类鼻疽伯克霍尔德杆菌以及出血热病毒埃博拉病毒、拉沙病毒的反应 和 SARS-CoV-2 的高防护设施中缺乏细胞分析仪阻碍了佐治亚大学研究人员的研究。 因此，该仪器将有助于利用高致病性生物体进行关键实验。 实现研究目标并维持 NIH 对生物医学研究的支持。