Combined Optical Tweezers-Fluorescence Super-Resolution Microscope for Single-Molecule Biophysical Studies

用于单分子生物物理研究的光镊-荧光超分辨率组合显微镜

• 批准号: 10177000
• 负责人: Ruben L Gonzalez
• 金额: $ 133.75万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177000
• 关键词: Area; Biochemical; Biomedical Research; Biophysics; Communities; Fluorescence; Funding; Future; Individual; Laboratories; Lasers; Microscope; New York City; Photons; Research; Research Personnel; Resolution; Scanning; Schools; United States National Institutes of Health; Universities; biophysical analysis; cost; experimental study; fluorescence microscope; instrument; instrumentation; laser tweezer; nanoscope; programs; single molecule

PROJECT SUMMARY In this application, we propose to acquire a combined optical tweezers-fluorescence super-resolution microscope for single-molecule biophysical studies from LUMICKS. The LUMICKS C-Trap will be placed in a shared instrumentation facility such that it will be available for use by the entire biomedical research community at Columbia University. Consequently, the C-Trap will impact a large number of current and future NIH-funded research programs at Columbia University. Specifically, the C-Trap will be installed, housed, operated, and maintained in the Columbia University Precision Biomolecular Characterization Facility (PBCF), a university- supported, staffed, shared instrumentation facility that will provide all Columbia University researchers with efficient, low-cost access to the C-Trap. The proposed C-Trap is a state-of-the-art instrument that will be equipped with quadrupule laser tweezers, a three-wavelength, single-photon-sensitivity laser scanning confocal fluorescence microscope, and stimulated emission depletion (STED) super-resolution fluorescence microscope (i.e., a STED nanoscope), thereby enabling the broadest range of research to be accommodated. There are currently no comparable instruments anywhere at Columbia University and there are only two C-Trap instruments in the entire New York City area. These two instruments are not located in a shared instrumentation facility and are instead located in the laboratory of individual researchers, where the instruments exclusively serve the experimental needs of the individual researcher’s laboratory and are therefore rendered unavailable to outside users. Moreover, these two instruments are configured differently from the requested instrument and have limited capabilities and, thus, cannot be used to perform the experiments proposed in the current application. Successfully establishing the proposed C-Trap in the PBCF is expected to advance the aims of more than 26 NIH-funded research programs spanning many departments and schools at Columbia University and representing an extremely broad range of biochemical, biophysical, and biomedical research programs.

项目概要 在本应用中，我们建议获得组合式光镊-荧光超分辨率显微镜 用于 LUMICKS 的单分子生物物理研究。 LUMICKS C-Trap 将放置在共享的环境中。 仪器设施，以便整个生物医学研究界可以使用 哥伦比亚大学研究发现，C-Trap 将影响大量当前和未来的 NIH 资助项目。 具体来说，C-Trap 将在哥伦比亚大学的研究项目中安装、安置、操作和运行。 保存在哥伦比亚大学精密生物分子表征设施（PBCF）中，该设施是一所大学- 支持、配备人员、共享仪器设施，将为所有哥伦比亚大学研究人员提供 高效、低成本地访问 C-Trap 拟议的 C-Trap 是一种最先进的仪器，将成为 配备四极激光镊子，三波长、单光子灵敏度激光扫描共焦 荧光显微镜和受激发射损耗（STED）超分辨率荧光显微镜 （即 STED 纳米显微镜），从而能够适应最广泛的研究。 目前哥伦比亚大学任何地方都没有类似的仪器，并且只有两台 C-Trap 整个纽约市地区的仪器 这两个仪器并不位于共享仪器中。 设施，而是位于个别研究人员的实验室中，其中的仪器专门用于 满足个别研究人员实验室的实验需求，因此无法使用 此外，这两种仪器的配置与所要求的仪器不同。 能力有限，因此不能用于执行当前提出的实验 在 PBCF 中成功建立拟议的 C-Trap 预计将推动更多目标的实现。 超过 26 个由 NIH 资助的研究项目，涵盖哥伦比亚大学和 代表了极其广泛的生物化学、生物物理和生物医学研究项目。