Kinetic Imaging Cytometer (KIC) for High Throughput Studies of Cellular Physiology

用于细胞生理学高通量研究的动态成像细胞仪 (KIC)

• 批准号: 10175806
• 负责人: MARK MERCOLA
• 金额: $ 52.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-26 至 2022-10-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175806
• 关键词: Biomedical Engineering; Cardiac Myocytes; Cardiovascular Diseases; Cardiovascular system; Cell physiology; Cells; Complications of Diabetes Mellitus; Development; Disease; Disease model; Electrophysiology (science); Ensure; Faculty; Faculty Recruitment; Funding; Goals; Grant; Heart failure; Image; Institutes; Institution; Kinetics; Laboratories; Maintenance; Medicine; Minor; Ophthalmology; Patient Care; Pediatrics; Physiological; Physiology; Play; Proteome; Research; Research Personnel; Resources; Robotics; Role; Science; Speed; Strategic Planning; System; Therapeutic; Time; United States National Institutes of Health; base; cancer therapy; drug development; experience; heart cell; improved; induced pluripotent stem cell; innovation; instrument; instrumentation; medical schools; operation; patch clamp; screening; senior faculty; stem cell model; whole genome

PROJECT SUMMARY/ABSTRACT: The Stanford Cardiovascular Institute (CVI) proposes the acquisition of a Vala Sciences’ IC200 Kinetic Imaging Cytometer (KIC) to be placed in a shared setting in the new Biomedical Innovations (BMI) Building that is centrally located on campus. The IC200 KIC is a cutting edge instrument that combines aspects of high content screening systems with high speed video imaging and cell-by-cell analysis features of physiological recording instrumentation such as patch clamp electrophysiology setups. No other type of instrument combines these features, and no comparable instrument is available on Stanford campus or in the vicinity. The instrument will be used for in-depth studies that probe disease mechanisms of excitable cells such as cardiomyocytes on an unprecedented whole genome and whole proteome level. The focus on mechanism-based understanding of disease, and ultimately the development of mechanism- based therapeutics, aligns with the long-term strategic plan of the Stanford CVI and School of Medicine, and is consistent with recent faculty recruitment. The capability of high throughput assessment of physiological function of heart cells plays a central role in this plan. The instrument will be managed by an experienced group with expertise in high content screening and disease modelling using induced pluripotent stem cell (iPSC)-based models. The CVI comprises more than 400 investigators and more than 125 faculty-led research groups and spans diverse departments including Medicine, Pediatrics, Ophthalmology and Biomedical Engineering. The Major and Minor Users highlighted in this proposal range from junior faculty who are just starting their own laboratories to established full professors with over 20 years of research experience. All the major users have NIH funding, and the collective benefit to NIH grants comprises over 80% of the anticipated user time of the instrument. The institution is committing considerable financial resources and dedicated space to ensure the maintenance and continued operation of the instrument, including its integration into a robotics facility. We expect that the IC200 will positively impact a broad cross section of cardiovascular and other diseases, notably heart failure and cardiovascular complications of diabetes and cancer treatments. In addition, the high throughput and physiology recording aspects of the IC200 will propel research towards our drug development and our translational goal of improving care of patients with cardiovascular disease.

项目概要/摘要： 斯坦福心血管研究所 (CVI) 提议收购 Vala Sciences 的 IC200 动态成像细胞仪 (KIC) 将放置在新生物医学中心的共享环境中 位于校园中心的创新 (BMI) 大楼是一栋尖端建筑。 将高内容筛选系统与高速视频相结合的仪器 生理记录仪器的成像和逐个细胞分析功能，例如 膜片钳电生理学设置没有其他类型的仪器结合了这些功能，并且 斯坦福大学校园或附近地区没有类似的仪器。 可用于探索可兴奋细胞疾病机制的深入研究，例如 心肌细胞在前所未有的全基因组和全蛋白质组水平上的关注。 基于机制的疾病理解，并最终发展机制- 基于疗法，符合斯坦福大学 CVI 和学院的长期战略计划 医学，并且与最近的师资招聘相一致 具有高吞吐量的能力。 心脏细胞生理功能的评估在该计划中发挥着核心作用。 将由在高内涵筛查和疾病方面具有专业知识的经验丰富的团队管理 使用基于诱导多能干细胞 (iPSC) 的模型进行建模 CVI 包含更多内容。 超过 400 名研究人员和超过 125 个由教师领导的研究小组，涵盖不同领域 科室包括医学、儿科、眼科和生物医学工程。 本提案中强调的主要和次要用户包括刚刚起步的初级教师 拥有自己的实验室，建立了具有20多年研究经验的教授。 所有主要用户都有 NIH 资助，NIH 资助的集体利益包括超过 仪器预期用户时间的 80%。 财政资源和专用空间，以确保维护和持续运行 该仪器，包括将其集成到机器人设施中，我们预计 IC200 将能够实现。 对心血管和其他疾病的广泛领域产生积极影响，特别是心力衰竭 以及糖尿病和癌症治疗的心血管并发症。 IC200 的吞吐量和生理学记录方面将推动我们的药物研究 的发展以及我们改善心血管疾病患者护理的转化目标。

项目成果

Genetic analysis and multimodal imaging identify novel mtDNA 12148T>C leading to multisystem dysfunction with tissue-specific heteroplasmy.

遗传分析和多模态成像发现了新的 mtDNA 12148T>C，导致多系统功能障碍和组织特异性异质性。

• 发表时间: 2023-11-05
• 作者: Belle, Kinsley;Kreymerman, Alexander;Vadgama, Nirmal;Ji, Marco H;Randhawa, Sandeep;Caicedo, Juan;Wong, Megan;Muscat, Stephanie P;Gifford, Casey A;Lee, Richard T;Nasir, Jamal;Young, Jill L;Enns, Gregory;Karakikes, Ioannis;Mercola, Mark;Wood
• 通讯作者: Wood