"Acquisition of a Multi-functional Cryo Scanning Electron Microscope (SEM)"

“购买多功能冷冻扫描电子显微镜 (SEM)”

• 批准号: 10175680
• 负责人: MARY ANN Y JABRA-RIZK
• 金额: $ 85.49万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2024-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175680
• 关键词: 3-Dimensional; Award; Baltimore; Biological; Biomedical Research; Communicable Diseases; Companions; Complement; Computer software; Core Facility; Diabetes Mellitus; Diagnostic; Electron Microscope; Electron Microscopy; Freezing; Funding; Grant; Hydration status; Hydrogels; Image; Information Storage; Institution; Kidney Diseases; Maintenance; Malignant Neoplasms; Maryland; Medical Device; Medical Device Designs; Microbial Biofilms; Microspheres; Minor; Nanotechnology; Nerve Degeneration; Obesity; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Services; Signal Transduction; Specimen; System; Temperature; Therapeutic; Tissues; Training; United States National Institutes of Health; Universities; Vaccine Research; Wages; base; bone; cost; design; drug development; hearing impairment; imaging facilities; instrument; instrumentation; light microscopy; programs; tendon development

PROJECT SUMMARY Acquisition of a multi-functional cryo scanning electron microscope: The Electron Microscopy Core Imaging Facility (EMCIF) of the University of Maryland Baltimore (UMB) requests funds to acquire an integrated cryo scanning electron microscope (SEM), the Apreo S LoVac from Thermo Fisher Scientific (formerly FEI Company). This multifunctional instrument is bundled with automated navigation and imaging analysis software and is capable of imaging delicate biological specimens at room temperature and in a frozen hydrated state. Companion programs are designed to correlate light microscopy signal with ultrastructural detail obtained by electron microscopy (EM), and to scan serial sections, thereby allowing imaging of a tissue specimen in 3-D. Eleven NIH funded investigators representing eight research groups studying a wide range of important biomedical research topics will be Major Users. The research domains of these Major users include cancer, obesity, diabetes, kidney diseases, infectious diseases, neural degeneration, hearing loss, bone/tendon development, drug development, medical device design, and diagnostic and therapeutic strategies that will all benefit from the capabilities of the new instrument. Five additional Minor Users will benefit from the enhanced resolution at both room and cryo temperatures to examine biofilms for vaccine research and hydrogels and microspheres for nanotechnology-based medical devices and therapeutic strategies. The requested instrument will be placed within the current EMCIF, a well- organized core facility that provides access to state-of-the-art EM instrumentation and training to over 150 researchers, trainees and staff each year. Strong institutional support provides ongoing support for Director and staff salaries, instrument service/maintenance and other EMCIF operating costs, new information storage/computing capacity, and a $100,000 contribution toward the purchase of the new instrument. Should this grant be awarded, the ultimate impact of this unique and versatile cryo SEM system will extend beyond the research projects listed in this proposal. Overall, this new capacity will complement existing imaging UMB capabilities and expand the capacity for biomedical research at UMB and regional institutions.

项目概要 购置多功能冷冻扫描电子显微镜： 马里兰大学巴尔的摩分校 (UMB) 的电子显微镜核心成像设施 (EMCIF) 请求资金购买集成冷冻扫描电子显微镜 (SEM)，Apreo S LoVac Thermo Fisher Scientific（原 FEI 公司）。该多功能仪器捆绑有自动化 导航和成像分析软件，能够在室内对精致的生物样本进行成像 温度和冷冻水合状态。配套程序旨在关联光学显微镜 信号与通过电子显微镜（EM）获得的超微结构细节，并扫描连续切片，从而 允许对组织样本进行 3D 成像。 11 名 NIH 资助的研究人员代表了 8 项研究 研究广泛的重要生物医学研究主题的团体将成为主要用户。研究 这些主要用户的领域包括癌症、肥胖、糖尿病、肾脏疾病、传染病、神经疾病 退化、听力损失、骨/肌腱发育、药物开发、医疗设备设计，以及 诊断和治疗策略都将受益于新仪器的功能。五 其他未成年人用户将受益于室温和低温下增强的分辨率 检查用于疫苗研究的生物膜以及用于基于纳米技术的医疗的水凝胶和微球 设备和治疗策略。所请求的仪器将放置在当前的 EMCIF 内，这是一个良好的 组织核心设施，为超过 150 名人员提供最先进的 EM 仪器和培训 每年研究人员、实习生和工作人员。强大的机构支持为主任提供持续支持 和员工工资、仪器服务/维护和其他 EMCIF 运营成本、新信息 存储/计算能力，并捐赠 100,000 美元购买新仪器。应该 如果获得这笔资助，这种独特且多功能的冷冻 SEM 系统的最终影响将超越 本提案中列出的研究项目。总体而言，这一新产能将补充现有的成像 UMB 能力并扩大 UMB 和区域机构的生物医学研究能力。