Toward a human adult brain cell atlas with single-cell technologies

利用单细胞技术构建人类成人脑细胞图谱

基本信息

批准号：
10165827
负责人：
JEROLD CHUN
金额：
$ 173.07万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-22 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10165827
关键词：
3-Dimensional Adult Age Animal Model Area Atlases Autopsy Biological Assay Brain Brain Diseases Brain region Brodmann&apos s area Cataloging Catalogs Cell Nucleus Cells Cerebral cortex Chromatin Clinical Cognitive Collection Complement component C1 Complex DNA Data Data Analyses Dimensions Disease Epigenetic Process Event Female Freezing Future Gene Expression Genes Genetic Transcription Goals Grant Hour Human In Situ Individual Interneurons Investigation MRI Scans Magnetic Resonance Imaging Maps Medulla Oblongata Metabolic Metencephalon Methods Midbrain structure Molecular Mus Neuroglia Neurons Neurotransmitters Pathway interactions Publishing RNA Reporting Research Personnel Resolution Sampling Source Spatial Distribution Spinal Cord Technology Telencephalon Transcript Writing base brain cell brain tissue cell type cognitive function diencephalon experience in situ imaging large scale data male multiple omics new technology next generation novel organizational structure sex single cell analysis single cell sequencing single cell technology transcriptome transcriptome sequencing

项目摘要

Abstract Human brain is an exceedingly complex network of spatially organized and functionally connected neurons imbedded in glia. In surpasses the mouse brain by three orders of magnitude in terms of sheer numbers of cells, and likely has a more complex organization structure relevant to human-specific cognitive functions. Defining a complete cell atlas of the human brain, including a full catalog of all cell types (i.e. all parts) and their spatial distribution, is a critical step towards understanding the human cognitive machine. In this project we will greatly expand our previous efforts towards building a complete and spatially resolved cell atlas of the human whole brain, using a combination of three novel technologies for scalable single-cell sequencing and in situ imaging. Novel computational approaches will also be developed for integration and analysis of data at these larger scales. We will systematically apply these methods to MRI-scanned human whole brains to produce reference cell maps for female and male brains, and construct a spatial multi-omic map of the human adult brain.

抽象的人脑是一个极其复杂的空间组织和功能网络嵌入神经胶质细胞中的连接神经元。超过小鼠大脑三个数量级就细胞的绝对数量而言，其数量级很大，并且可能具有更复杂的组织与人类特定认知功能相关的结构。定义完整的细胞图谱人脑，包括所有细胞类型（即所有部分）及其空间分布的完整目录，是理解人类认知机器的关键一步。在这个项目中，我们将极大地扩展我们之前的努力，以建立一个完整的、人类全脑的空间分辨细胞图谱，使用三种新颖的组合可扩展的单细胞测序和原位成像技术。新颖的计算还将开发对这些更大规模的数据进行整合和分析的方法。我们将系统地将这些方法应用于 MRI 扫描的人类全脑，以产生参考女性和男性大脑的细胞图，并构建空间多组学图人类成人的大脑。