GROWTH FACTORS IN HUMAN FETAL OOGENESIS

人类胎儿卵子发生中的生长因素

• 批准号: 2194542
• 负责人: JOHN YEH
• 金额: $ 8.15万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2194542
• 关键词: angiogenesis; apoptosis; autocrine; cell growth regulation; cellular pathology; epidermal growth factor; fibroblast growth factor; gene expression; growth factor; growth factor receptors; human fetus tissue; human tissue; immunocytochemistry; in situ hybridization; insulin receptor; insulinlike growth factor; messenger RNA; oogenesis; ovary; paracrine; polymerase chain reaction; transforming growth factors; transmission electron microscopy

The transformation of germ cells into primordial follicles in the human fetus, then into the follicles of the ovary, represents an important and relatively little understood process. Although microscopic descriptions of the changes in the number of oogonia are available, the actual factors which may regulate cell growth, angiogenesis, apoptosis and pattern formation are unclear. The broad long-term objectives of this proposal are (1) to clarify the role of peptide growth factors as signals/agents for growth and differentiation in human fetal ovaries and (2) to understand how peptide growth factors may contribute to abnormalities in adults ovaries. The research proposed for this application is intended to accomplish these goals: . Specific Aim 1. Quantitative analysis of mRNA for growth factors and their receptors during the process of human fetal oogenesis. . Specific Aim 2. Localization by immunohistochemistry and in situ hybridization of growth factors and their receptors in human fetal ovarian development. . Specific Aim 3. To investigate the gene expression of growth factors and their receptors and to investigate apoptosis in chromosomally abnormal fetal ovaries. . Specific Aim 4. Morphological analysis of development of oogonia and surrounding cells in fetal ovaries.

生殖细胞转化为人类的原始卵泡 胎儿，然后进入卵巢的卵泡，代表一个重要的 相对较少的理解过程。 虽然微观描述 可用的oogonia数量变化，实际因素 可能调节细胞生长，血管生成，凋亡和模式 形成不清楚。 该提议的广泛长期目标 是（1）阐明肽生长因子作为信号/药物的作用 用于人类胎儿卵巢的生长和分化和（2） 了解肽生长因子如何导致异常 成人卵巢。 该应用程序提出的研究旨在 实现这些目标： 。特定目的1。对生长因子的mRNA定量分析和 它们的受体在人类胎儿卵子发生过程中。 。特定目标2。免疫组织化学和原位定位 生长因子及其受体在人胎儿的杂交 卵巢发展。 。特定目的3。研究生长因子的基因表达 及其受体，并研究染色体的凋亡 异常的胎儿卵巢。 。特定目的4。对Oogonia和Ogonia和 胎儿卵巢中的细胞。