Laboratory Sciences Core (Core D)

实验室科学核心（核心 D）

基本信息

批准号：
10153673
负责人：
Spyros A Kalams
金额：
$ 50.99万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-04-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10153673
关键词：
Academic Medical Centers Acquired Immunodeficiency Syndrome Address Adipose tissue Administrative Supplement Adoption Area B-Cell Antigen Receptor B-Lymphocytes Basic Science Biological Assay Biomass Cell Separation Cell physiology Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Clinical Clinical Sciences Collaborations Consultations Core Facility Cryopreservation DNA DNA sequencing Discipline Disease Equipment Flow Cytometry Funding Future Gene Expression Profiling Genomics Grant HIV HIV Genome Health Immunology In Vitro Individual Laboratories Leadership Lymphocyte Measures Metagenomics Methodology Mission Nucleic Acids Pathogenesis Performance Peripheral Blood Mononuclear Cell Phenotype Plasma Population Procedures Proteins Protocols documentation RNA Receptor Cell Recording of previous events Research Research Design Research Personnel Resources Ribosomal RNA Role Saliva Sampling Schedule Science Sequence Analysis Services Skin Sorting - Cell Movement Specimen Stains System T cell receptor repertoire sequencing T-Lymphocyte Techniques Technology Tennessee Tissues Training Translational Research Viral Genome Work career development design digital genetic variant genomic platform host microbiome human genomics innovation integration site laboratory experience medical schools microbial microbial genomics microbiome analysis microbiome sequencing nano-string single cell sequencing success synergism transcriptomics viral genomics

项目摘要

Laboratory Sciences Core – PROJECT SUMMARY The CFAR Laboratory Sciences (LSC) provides services specifically designed to meet the needs of HIV clinical, translational and basic science researchers. Over the past grant cycle, we established multiple services for CFAR investigators, with an easily accessible scheduling and chargeback system, regardless of investigators' prior level of laboratory experience or training. These include cell isolation and cryopreservation protocols for PBMCs, adipose tissue, and skin. We developed cell staining protocols for analytical flow cytometry to evaluate the phenotype and function of B and T lymphocytes, and flow sorting assays to bulk sort populations of lymphocytes, or to perform single-cell sorting for detailed transcriptomic analyses. Vanderbilt has long prioritized institutional support for shared core facilities and invested accordingly. The services that the LSC established over the prior funding cycle were carefully designed to address needs unmet by other non-CFAR Vanderbilt core facilities, while simultaneously taking advantage of the impressive range of institutional cores that are equally available to investigators at Vanderbilt and Meharry. Our successes have allowed us to anticipate the future needs of this CFAR. An institutional core, VANTAGE (VANderbilt Technologies for Advanced Genomics), has significantly enhanced our ability to analyze single cells, including B cell receptor and T cell receptor sequencing, and RNA transcriptional profiling. VANTAGE has also designed assays to perform whole HIV genome, including integration site, sequencing. The LSC, in addition to providing its own services, will function as a concierge service. We will help investigators design appropriate flow cytometry panels, and sort cells, and leverage VANTAGE to perform sequencing and analysis. The mission of the LSC is to continue to provide sustainable leadership and services specifically responsive to the needs of HIV investigators, so that these services are used in ways unimaginable without CFAR. The LSC will work closely with CFAR investigators to design assays, facilitate assay performance, and help interpret results. The LSC will promote basic, clinical and translational HIV research through two Specific Aims: 1) To provide expertise, consultation, assay performance, and training in immunology techniques including those that require BSL3 procedures. These include study design and implementation of state-of-the-art assays to measure phenotype and function of cell subsets relevant to HIV and to quantitate HIV protein and nucleic acid in plasma samples and in vitro culture. 2) To provide concierge level service for genomics research relevant to HIV. Services include access to single-cell transcriptomics technologies, viral genome evolutionary sequence analysis, and host microbiome analysis relevant to HIV disease pathogenesis.

实验室科学核心——项目摘要 CFAR 实验室科学 (LSC) 提供专为满足 HIV 临床、在过去的资助周期中，我们为转化和基础科学研究人员建立了多项服务。 CFAR 调查员，拥有易于访问的调度和退款系统，无论调查员的情况如何先前的实验室经验或培训水平，包括细胞分离和冷冻保存方案。我们开发了用于分析流式细胞仪评估的细胞染色方案。 B 和 T 淋巴细胞的表型和功能，以及对 B 和 T 淋巴细胞进行批量分类的流式分选测定淋巴细胞，或进行单细胞分选以进行详细的转录组分析，范德比尔特长期以来一直优先考虑。为共享核心设施提供机构支持并相应投资 LSC 建立的服务。在之前的资助周期中经过精心设计，以解决其他非 CFAR 范德比尔特核心无法满足的需求设施，同时利用一系列令人印象深刻的机构核心，这些核心机构同样具有范德比尔特和梅哈里的调查人员可以使用我们的成功使我们能够预测未来。机构核心 VANTAGE（范德比尔特先进基因组学技术）拥有满足这一 CFAR 需求的能力。显着增强了我们分析单细胞的能力，包括B细胞受体和T细胞受体测序， RNA 转录谱分析还设计了用于执行整个 HIV 基因组的检测方法，包括。 LSC 除了提供自己的服务外，还将充当礼宾服务。我们将帮助研究人员设计合适的流式细胞仪面板、对细胞进行分类并利用。 VANTAGE 进行测序和分析 LSC 的使命是继续提供可持续的服务。专门满足艾滋病毒调查人员需求的领导和服务，以便这些服务得到利用如果没有 CFAR，LSC 将与 CFAR 研究人员密切合作来设计检测方法，促进检测性能，并帮助解释结果。LSC 将促进基础、临床和转化。 HIV 研究通过两个具体目标：1) 提供专业知识、咨询、检测性能和培训免疫学技术，包括需要 BSL3 程序的技术，其中包括研究设计和实施最先进的检测方法来测量与 HIV 相关的细胞亚群的表型和功能定量血浆样本和体外培养中的 HIV 蛋白和核酸 2) 提供礼宾级别。与 HIV 相关的基因组学研究服务服务包括获取单细胞转录组学。与 HIV 相关的技术、病毒基因组进化序列分析和宿主微生物组分析疾病发病机制。