VESTIBULAR REGULATION OF RESPIRATORY MUSCLE ACTIVITY

呼吸肌活动的前庭调节

• 批准号: 2128107
• 负责人: ALAN D MILLER
• 金额: $ 25.83万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2128107
• 关键词: brain stem; cats; decerebration; electrostimulus; experimental brain lesion; head movements; laryngeal nerves; medulla oblongata; neuronal transport; posture; pulmonary respiration; respiratory muscles; vagus nerve; vestibular apparatus; vestibular nerve; vestibular nuclei

The vestibular system produces a number of compensatory responses to accelerations imposed upon the head. Probably the least understood of these are vestibular influences on thoracic and abdominal "respiratory" muscles which also have postural and cardiovascular functions. The goal of this project is to determine both the functional significance of vestibular-evoked respiratory muscle responses and the neural substrate that produces these responses. Experiments will be conducted using the cat, for which there is considerable relevant background information concerning the vestibular and respiratory systems. Two types of experiments will be performed to gain insight into the functional significance of vestibular-respiratory responses, using the decerebrate cat preparation. First, the extent of the responses will be examined. In particular, we will determine if the activity of the muscles of the upper airway, like that of the respiratory muscles of the thorax and abdomen, is influenced by electrical stimulation of the vestibular nerve. Second, natural vestibular stimulation in multiple vertical planes and in the horizontal plane will be used to determine the directional sensitivity and dynamics of the respiratory muscle response. The neural substrate that produces the vestibulo-respiratory response will be examined using three types of experiments. First, the anterograde neuroanatomical tracer Phaseolus vulgaris leucoagglutinin (PHA-L) will be iontophoretically injected into the medial and adjacent inferior vestibular nuclei to determine which areas containing respiratory neurons may receive direct vestibular inputs. The medial and inferior nuclei were chosen for study since they are known to be essential for mediating vestibulo-respiratory responses. Second, the responses of medullary respiratory neurons to electrical and natural vestibular input will be determined. Our preliminary data indicate that at least some of these neurons are responsive to vestibular stimulation. Finally, medullary respiratory groups will be inactivated using chinook acid microinjections to determine their importance in mediating vestibulo-respiratory responses.

前庭系统对 施加在头上的加速度。 可能最了解的 这些是对胸腔和腹部“呼吸道”的前庭影响 肌肉也具有姿势和心血管功能。 目标 这个项目的是确定功能意义 前庭诱发的呼吸肌反应和神经底物 产生这些反应。 实验将使用 猫，有相关的背景信息 关于前庭和呼吸系统。 将进行两种类型的实验，以了解 前庭呼吸反应的功能意义，使用 脑猫的准备。 首先，回应的程度将是 检查。 特别是，我们将确定肌肉的活性是否 上呼吸道，就像胸腔的呼吸道肌肉一样 腹部受到前庭的电刺激的影响 神经。 其次，多个垂直平面的天然前庭刺激 在水平面中将用于确定方向性 呼吸肌反应的敏感性和动力学。 产生前庭呼吸反应的神经底物将 使用三种类型的实验检查。 首先，如今 神经解剖示踪剂叶状果素蛋白（PHA-L）将是 离子po养注射到内侧和邻近的下 前庭核确定哪些区域含有呼吸神经元 可能会收到直接的前庭输入。 内侧和下核是 选择进行研究，因为它们是介导至关重要的 前庭呼吸反应。 其次，髓质的反应 对电气和天然前庭输入的呼吸神经元将是 决定。 我们的初步数据表明至少其中一些 神经元对前庭刺激有反应。 最后，髓 使用奇努克酸的显微注射将呼吸组灭活 确定它们在调解前庭呼吸器中的重要性 回答。