HORMONAL AND IONIC CONTROL OF METABOLISM

新陈代谢的激素和离子控制

• 批准号: 2137386
• 负责人: HOWARD RASMUSSEN
• 金额: $ 14.92万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2137386
• 关键词: 3T3 cells; Gastropoda; adrenal glands; adrenocorticotropic hormone; angiotensin II; biological information processing; brush border membrane; calcium channel; calcium metabolism; calmodulin; cholera toxin; cow; cyclic AMP; cytoplasm; drug receptors; electron microscopy; erythrocyte membrane; gastrointestinal absorption /transport; gel filtration chromatography; glucose transport; hormone regulation /control mechanism; human tissue; hydrogen; intestinal mucosa; intracellular transport; ion transport; ionophores; laboratory rabbit; laboratory rat; liver cells; membrane transport proteins; mitochondria; muscle contraction; myosins; peptide hormone; phenothiazines; radiotracer; sodium

The major aims of the proposed research is to more fully define the mechanisms by which CA2+ regulates cell function during such processes as the hormonal and ionic regulations of aldosterone secretion, from adrenal glomerulosa cells. Cell proliferation of cultured 3T3 cells, and leukocytes function. Specific aims include an elucidation of the temporal patterns of protein phosphorylation, inositol lipid metabolism, Ca2+ metabolism, and protein kinase C function during angiotensin II and bombesin-mediated response in, respectively, glomerulosa and 3T3 cells. The role of type II CaM-dependent protein kinase in the initial phase of cellular response; the immunocytochemical localization of the C-kinase and the possible role of M-kinase in sustained cellular response; the mechanism by which information is conveyed from cell surface to cell interior via the C-kinase branch of the Ca2+ messenger system during the sustained phase of cellular response focusing particularly on changes in Na+/H+ exchange and possible protein kinase cascades; the molecular basis of memory in the C-kinase-mediated phase of cellular response; and a reexamination of the temporal pattern of events in the cAMP messenger system during ACTH-mediate aldosterone secretion from adrenal glomerulosa cells as the system in which to explore these issues. Methods will include studies of Ca2+ fluxes and measurements of intracellular free Ca2+ using both fura 2 and aequorin as indicators; studies of the turnover of inositol lipids and inositols phosphates by both chemical and radio- isotope methods; 2-D gel electrophoresis of the phosphoproteins obtained from extracts of cells; and both light and EM immunocytochemical localization of C-kinase. The problem under investigation is of broad biological interest, and has implications for a wide range of biological phenomena. A better understanding of Ca2+ messenger system function is likely to alter our views of cell growth and proliferation, peptide and steroid hormone secretion, and smooth muscle contraction.

拟议研究的主要目的是更全面地定义 CA2+ 调节细胞功能的机制 诸如荷尔蒙和离子调节等过程 醛固酮由肾上腺肾小球细胞分泌。 细胞 培养的 3T3 细胞的增殖和白细胞功能。 具体目标包括阐明时间模式 蛋白质磷酸化、肌醇脂质代谢、Ca2+ 血管紧张素 II 期间的代谢和蛋白激酶 C 功能 和铃蟾肽介导的反应，分别在肾小球和 3T3细胞。 II 型 CaM 依赖性蛋白激酶在 细胞反应的初始阶段；免疫细胞化学 C-激酶的定位和 M-激酶的可能作用 持续的细胞反应；信息传递的机制 通过 C 激酶从细胞表面传送到细胞内部 持续期 Ca2+ 信使系统的分支 细胞反应尤其关注 Na+/H+ 的变化 交换和可能的蛋白激酶级联；分子 C-激酶介导的细胞阶段记忆的基础 回复;以及对事件的时间模式的重新审视 在 ACTH 介导的醛固酮过程中，cAMP 信使系统中 肾上腺肾小球细胞分泌的系统 来探讨这些问题。 方法将包括 Ca2+ 研究 使用两者的流量和细胞内游离 Ca2+ 的测量 Fura 2和水母发光蛋白作为指示剂；营业额研究 肌醇脂质和肌醇磷酸酯通过化学和放射 同位素方法；磷蛋白的二维凝胶电泳 从细胞提取物中获得；以及光和电磁波 C-激酶的免疫细胞化学定位。 下的问题 研究具有广泛的生物学意义，并且具有影响 适用于广泛的生物现象。 更好的理解 Ca2+ 信使系统功能的研究可能会改变我们的观点 细胞生长和增殖、肽和类固醇激素 分泌和平滑肌收缩。

项目成果

Influence of staurosporine on glucose-mediated and glucose-conditioned insulin secretion.

星形孢菌素对葡萄糖介导和葡萄糖调节的胰岛素分泌的影响。

• DOI: 10.1042/bj2790807
• 发表时间: 1991
• 期刊: The Biochemical journal
• 作者: Zawalich,WS;Zawalich,KC;Ganesan,S;Calle,R;Rasmussen,H
• 通讯作者: Rasmussen,H

Ca(2+)-cyclic AMP interactions in sustained cellular responses.

• DOI: 10.1002/9780470514207.ch7
• 发表时间: 1992
• 期刊: Ciba Foundation symposium
• 作者: H. Rasmussen;C. Isales;S. Ganesan;R. Calle;W. Zawalich

Calcium as intracellular messenger: from simplicity to complexity.

• DOI: 10.1016/b978-0-12-152831-7.50003-2
• 发表时间: 1990
• 期刊: Current topics in cellular regulation
• 作者: H. Rasmussen;J. Rasmussen

Expression of inducible nitric oxide synthase in breast cancer correlates with metastatic disease.

• 发表时间: 1997-07
• 期刊: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
• 作者: Alfonso Dueñas-González;Carlos M. Isales;M. M. Abad-Hernández-M.;Rogelio González-Sarmiento;O. Sangueza;J. Rodriguez-Commes

Cycling of Ca2+ across the plasma membrane as a mechanism for generating a Ca2+ signal for cell activation.

Ca2 穿过质膜的循环是产生细胞激活 Ca2 信号的机制。

• DOI: 10.1111/j.1749-6632.1989.tb12492.x
• 发表时间: 1989
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Rasmussen,H;Barrett,P;Zawalich,W;Isales,C;Stein,P;Smallwood,J;McCarthy,R;Bollag,W
• 通讯作者: Bollag,W