NORMAL AND NEOPLASTIC PITUITARY TISSUE

正常和肿瘤性垂体组织

• 批准号: 2091029
• 负责人: Ricardo Vincent Lloyd
• 金额: $ 20.88万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2091029
• 关键词: RNA biosynthesis; adenoma; autoradiography; cell differentiation; cell growth regulation; cell type; cellular oncology; electron microscopy; estrogen receptors; growth factor; hormone receptor; hormone regulation /control mechanism; hormone related neoplasm /cancer; human tissue; hypothalamic pituitary axis; hypothalamus; immunochemistry; in situ hybridization; messenger RNA; neoplastic cell; neural cell adhesion molecules; phorbols; pituitary gland; pituitary neoplasms; prolactin; radioimmunoassay; receptor binding; secretion; tissue /cell culture; transcription factor

Although pituitary neoplasms are associated with a great deal of morbidity because of excessive hormone production and/or as space occupying lesions, very little is known about the mechanisms regulating the development of pituitary hyperplasia and neoplasia in humans. The regulation of hormone production and differentiation in normal and neoplastic human pituitary gland tissues will be investigated using morphological, immunochemical, biochemical and in situ hybridization methods. Morphological studies at the light and electron microscopic level, immunochemical methods with specific antibodies and in situ hybridization analyses with oligonucleotide, cDNA and riboprobes will be used to study normal pituitaries, prolactin (PRL), growth hormone (GH)-producing and null cell or undifferentiated adenomas. Cell culture on extracellular matrix with serum free media will be used to analyze the effects of specific hypothalamic hormones such as thyrotropin releasing hormone, gonadotropin releasing hormone, corticotropin releasing hormone and somatostatin, as well as phorbol esters on hormone production and differentiation. Receptor autoradiography and biochemical analysis for specific receptors will be performed in these tissues and correlated with the effects of various secretagogues on cell differentiation. The role of specific growth factors, such as epidermal growth factor and basic fibroblast growth factor on pituitary cell differentiation will be examined. The regulation of estrogen receptor, chromogranins and pituitary transcription factor messenger RNA levels in normal and neoplastic pituitary cells will be analyzed. The long-term objectives are to understand the morphological and biochemical differences and similarities between normal and neoplastic human pituitary tissues and to gain more insight into the biology of normal and neoplastic pituitary cells. The data from these studies may lead to a better understanding of the biology of normal and neoplastic pituitary cells in humans and to the development of methods to treat these tumors more effectively.

尽管垂体肿瘤与大量发病有关 由于激素的产生过多和/或作为占据的空间病变， 关于调节发展的机制知之甚少 人类的垂体增生和肿瘤。 调节激素产生和正常和分化的调节 肿瘤的人垂体组织将使用 形态学，免疫化学，生化和原位杂交 方法。光和电子显微镜水平的形态学研究， 具有特定抗体和原位杂交的免疫化学方法 将使用寡核苷酸，cDNA和核糖核酸分析来研究 正常垂体，催乳素（PRL），生长激素（GH）产生和无效 细胞或未分化的腺瘤。细胞外基质上的细胞培养 使用无血清的培养基将用于分析特定的影响 下丘脑激素，例如甲状腺激素释放激素，促性腺激素 释放激素，皮质激素释放激素和生长抑素，为 以及佛波酯在激素产生和分化方面。受体 特定受体的放射自显影和生化分析将是 在这些组织中进行，与各种效果相关 细胞分化的促分子。特定增长因素的作用， 例如表皮生长因子和基本成纤维细胞生长因子 将检查垂体细胞分化。雌激素的调节 受体，染色体蛋白和垂体转录因子Messenger RNA 将分析正常和肿瘤垂体细胞中的水平。这 长期目标是了解形态学和生化 正常和肿瘤的人垂体之间的差异和相似之处 组织并更多地了解正常和肿瘤的生物学 垂体细胞。这些研究的数据可能会导致更好 了解正常和肿瘤垂体细胞的生物学 人类和开发方法来治疗这些肿瘤 有效地。