Molecular mechanisms of pathogenicity of rinderpest virus

牛瘟病毒致病性的分子机制

• 批准号: 10044221
• 负责人: YAMANOUCHI Kazuya
• 金额: $ 8.64万
• 依托单位: Nippon Institute for Biological Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044221/
• 关键词: Rinderpest; Pathogenicity; Reverse genetics; Immune mechanism; Recombinant vaccine; ヘルパーT細胞; ISCOMワクチン; 防御免疫; 細胞性免疫; 細胞傷害性T細胞; 免疫抑制

System to recover infectious rinderpest virus (RPV) from cDNA copy of the virus genome was established on the RBOK strain of RPV, and role of virus gene in the pathogenicity was examined. The RBOK that is widely used as an attnuated vaccine does not show virulence in rabbits, whereas the L strain, another attenuated RPV, shows high virulence in rabbits. Involvement of the H gene in such difference in the susceptibility of rabbits was examined by construction chimeric RBOK virus containing the H gene of the RPV-L strain. The results indicate that not only the H gene but also the other genes may be involved in the expression of virulence in rabbits.

在RPV RBOK株上建立了从病毒基因组cDNA拷贝中回收传染性牛瘟病毒(RPV)的系统，并检查了病毒基因在致病性中的作用。广泛用作减毒疫苗的 RBOK 对兔子没有表现出毒力，而另一种减毒 RPV L 株对兔子表现出高毒力。通过构建含有RPV-L毒株的H基因的嵌合RBOK病毒来检查H基因在兔的易感性差异中的参与。结果表明，不仅H基因，其他基因也可能参与兔毒力的表达。

项目成果

山内一也: "Cell-mediated immune responses incattle vaccinated with a recombinant vaccinia"J.gen.Virol. 80. 1627-1634 (1999)

Kazuya Yamauchi：“重组牛痘疫苗接种中的细胞介导的免疫反应”J.gen.Virol。 80. 1627-1634 (1999)

山内一也: "Rinderpest virus ISCOM vaccine induces protection against virulent RPV challenge"Vaccine. (In press). (2001)

Kazuya Yamauchi：“牛瘟病毒 ISCOM 疫苗可诱导抵抗有毒 RPV 攻击”疫苗（2001 年出版）。

山内一也: "Rinderpest virus ISCOM vaccine induces protection against virulent RPV challenge"Vaccine. (In press). (2001)

Kazuya Yamauchi：“牛瘟病毒 ISCOM 疫苗可诱导抵抗有毒 RPV 攻击”疫苗（2001 年出版）。

山内一也: "Long-term protective immunity to rinderpest in cattle following a single vaccination with recombinant vaccinia virus"J. Gen. Virol.. 81. 1439-1446 (2000)

Kazuya Yamauchi：“重组痘苗病毒单次接种后牛对牛瘟的长期保护性免疫力”J. Gen. Virol.. 81. 1439-1446 (2000)

Kobune, F3: "Recovery of pathogenic measles virus from cloned cDNA"J.Virol.. 74. 6643-6647 (2000)

Kobune, F3：“从克隆的 cDNA 中回收致病性麻疹病毒”J.Virol.. 74. 6643-6647 (2000)