INTERCELLULAR COMMUNICATION AND CANCER

细胞间通讯与癌症

• 批准号: 2086249
• 负责人: WERNER R LOEWENSTEIN
• 金额: $ 24.41万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1994-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2086249
• 关键词: carcinogenesis; cell cell interaction; cell growth regulation; cellular oncology; enzyme activity; fluorescent dye /probe; genetic manipulation; immunocytochemistry; intercellular connection; membrane channels; membrane permeability; neoplasm /cancer genetics; phosphorylation; recombinant DNA; tissue /cell culture; western blottings

The long-term objective of this research program is to investigate the role of junctional cell-to-cell communication in growth control and the role of this communication in the etiology of cancer. In the present project, it will be investigated whether the growth of certain communication-deficient transformed cells can be corrected my manipulating the genes encoding the protein of the cell-cell membrane channels, the intercellular conduits of this communication; and, conversely, whether transformation can be induced by mutant genes for this protein. The role of protein kinase C in the regulation of communication and growth will be investigated. By use of mutant genes encoding kinases of enhanced enzyme activity, a correlated study will be undertaken on the kinase effects on communication, on the state of phosphorylation of the channel protein, and on growth. The main experimental approaches involve construction of recombinant DNAs, standard DNA and RNA analyses, immunoblottings, probings of junctional permeability with microinjected fluorescent tracers, analyses of protein phosphorylation including phosphoaminoacid analysis, and assays of cellular growth.

该研究计划的长期目标是调查 连接细胞对细胞通信在生长控制中的作用和 这种交流在癌症病因中的作用。 现在 项目，将研究某些人的增长 通信缺陷转化的细胞可以纠正我的操纵 编码细胞细胞膜通道蛋白质的基因， 这种交流的细胞间管道；相反，是否 转化可以由该蛋白的突变基因诱导。 角色 蛋白激酶C在传播和生长调节中的蛋白质C将是 调查。 通过使用编码增强酶激酶的突变基因 活动，将就激酶对激酶的影响进行相关研究 通信，在通道蛋白的磷酸化状态下，以及 关于增长。 主要的实验方法涉及建造 重组DNA，标准DNA和RNA分析，免疫印迹，探测 与微注射荧光示踪剂的连接渗透性的 蛋白质磷酸化的分析，包括磷酸氨基酸分析， 和细胞生长的测定。