MOLECULAR BIOLOGY OF MOT AND THE CHE GENE PRODUCTS

MOT 的分子生物学和 CHE 基因产品

• 批准号: 2060822
• 负责人: PHILIP MATSUMURA
• 金额: $ 21.88万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2060822
• 关键词: analytical method; antibody; biological signal transduction; cell motility; chemotaxis; developmental genetics; flagellum; gene expression; genetic manipulation; genetic transcription; membrane proteins; molecular cloning; mutant; nucleic acid sequence; phosphorylation; physical chemical interaction; protein biosynthesis; protein signal sequence; protein structure function; regulatory gene

Signal transduction in bacterial chemotaxis is arguably the best understood of the two-component signalling system first described by Ausubel (Nixon et al. 1986). Therefore, the understanding of the underlying mechanisms will have a great impact in many other examples of response to environmental signals. A long term goal of this application is characterization of the physical interactions and submolecular architecture of the chemotaxis components. The physical protein-protein interactions are being identified by the isolation of complexes of the chemotaxis proteins. These complexes are being isolated by antibody precipitation and protein affinity chromatography. The role of these complexes in the signalling process will be assessed by examining the composition of these complexes in different mutant backgrounds and different signalling states. Changes in biochemical properties such as phosphorylation will also be examined with respect to changes in the composition of these complexes. The elucidation of the submolecular architecture of these proteins will continue by using a combination of genetic selection, complex isolation and structural chemistry to map sites of interaction with other proteins and sites of function. This novel combination of molecular genetics and structural chemistry will produce testable hypotheses of protein structure and function which could not have been predicted from a purely structural or genetic approach. The other long term goal of this application is characterization of the growth-phase regulated expression of the flagellar regulon cascade. In particular, the roles of the positive trans-acting regulators, FlhD and FlhC, will be studied and their potential involvement in other postexponentially regulated phenomena will be further explored.

细菌趋化性中的信号转导可以说是最好的理解 Ausubel首先描述的两个组件信号传导系统（Nixon et al。 1986）。 因此，对基本机制的理解将 在许多其他对环境的反应的例子中都有很大的影响 信号。 该应用的长期目标是表征物理 趋化成分的相互作用和分子结构。 物理蛋白 - 蛋白质相互作用正在通过 分离趋化蛋白的复合物。 这些复合物是 通过抗体沉淀和蛋白质亲和力分离 色谱法。 这些复合物在信号传导过程中的作用将 可以通过检查不同的复合物的组成来评估 突变背景和不同的信号态。 生化的变化 还将检查诸如磷酸化之类的特性 这些复合物的组成变化。 阐明 这些蛋白质的分子结构将通过使用 遗传选择，复杂分离和结构的结合 化学以绘制与其他蛋白质和位点相互作用的位点 功能。 这种新颖的分子遗传学和结构的组合 化学将产生可检验的蛋白质结构和 无法通过纯粹的结构或 遗传方法。 此应用的另一个长期目标是表征 生长期调节鞭毛调节级联的表达。 在 特别是正面跨性调节器，FLHD和 FLHC将进行研究，并可能参与其他 指数后调节现象将进一步探讨。