REDUCED CALORIES, PROLIFERATION, IMMUNITY, CANCER, AGING

减少热量、增殖、免疫力、癌症、衰老

• 批准号: 2049186
• 负责人: ROBERT A GOOD
• 金额: $ 19.35万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2049186
• 关键词: REDUCED; CALORIES; PROLIFERATION; IMMUNITY; CANCER

DESCRIPTION (Investigator's Abstract): The main purpose of the proposed studies is to determine whether a major mechanism of action of Chronic Energy Intake Restriction (CEIR) to both prolong life and health by inhibiting development of diseases of aging is to down-regulate (control) rapid cell replication. To this end, the investigators propose to compare the influence of CEIR on replication of cells in each of the major rapidly replicating cell systems in mice of each of several autoimmune-prone strains. It is further proposed to study in some detail the influence of CEIR on the extraordinary compensatory burst of cell replication that follows partial hepatectomy and to determine whether CEIR influences the initiation, ongoing replication and cessation of this replicative burst. Finally, it will be determined whether CEIR inhibits either or both the benign proliferation which occurs in the Harderian gland or the malignancies of breast, lymphoid tissue or salivary gland which develop in mice transgenic for the Ha-ras oncogene that is driven by murine mammary tumor virus (MMTV) LTR, or the lymphomas and breast tumors which occur in mice transgenic for the c-myc gene driven by MMTV LTR, or the lymphomas, which occur in the mice transgenic for the c-myc gene driven by immunoglobulin gene LTR, or the lymphomas, breast tumor and salivary gland tumors which occur in the F1 hybrids between mice transgenic for the above Ha-ras MMTV LTR and mice transgenic for c-myc MMTV LTR. Also proposed to be determined is whether or not prolactin levels which the investigators can lower greatly by CEIR, and can raise or lower at will by pituitary transplantation or treatment by daily I.P. injections of CV-205-502, represent a crucial influence on each of these forms of cell proliferation. The applicants will also seek to define the controls of the proliferative processes that are influenced by CEIR through analyses of gene expression at the mRNA level for what are deemed to be the most likely crucial proto-oncogenes, transoncogenes and/or growth factor genes involved in control of these different proliferative processes. These investigations should contribute significantly to understanding how proliferative processes of cells and proliferative processes in cellular regeneration that may be involved in numerous diseases of aging can be influenced and controlled by dietary intervention. Another aim of the proposal is to learn how CEIR controls premalignant and malignant proliferations that are associated with expression of certain proto-oncogenes. These investigations could become central issues in efforts to extend life and health in humans by dietary intervention.

描述（研究者的摘要）：提议的主要目的 研究是确定慢性的主要作用机理 能源摄入限制（CEIR）延长寿命和健康 抑制衰老疾病的发展是下调（对照） 快速的细胞复制。 为此，调查人员建议比较 CEIR对每个主要主要迅速复制细胞复制的影响 复制几种自身免疫性易免疫的小鼠中的细胞系统 菌株。 进一步提议详细研究 CEIR关于细胞复制的非凡补偿性爆发 遵循部分肝切除术，并确定CEIR是否影响 启动，持续的复制和这种复制爆发的停止。 最后，将确定CEIR是抑制的 良性扩散发生在硬腺或 乳腺癌，淋巴组织或唾液腺的恶性肿瘤发生 由鼠乳腺驱动的HA-RAS癌基因的小鼠转基因 肿瘤病毒（MMTV）LTR，或发生在 由MMTV LTR或淋巴瘤驱动的C-MYC基因的小鼠转基因 在小鼠中发生的C-MYC基因的转基因中发生 免疫球蛋白基因LTR或淋巴瘤，乳腺肿瘤和唾液腺 上述小鼠转基因之间发生的F1杂种中发生的肿瘤 HA-RAS MMTV LTR和C-MYC MMTV LTR的小鼠转基因。 也提出了 可以确定研究者是否催乳素水平 CEIR可以大大降低，并且可以通过垂体而随意升高或降低 每日i.p.移植或治疗注射CV-205-502， 代表对每种形式的细胞增殖形式的关键影响。 申请人还将寻求定义增生性的控制 通过基因表达分析受CEIR影响的过程 在mRNA水平上，认为最有可能是最可能的 涉及的原始基因，跨结构和/或生长因子基因 控制这些不同的增殖过程。 这些调查 应该有助于理解如何增殖 细胞的过程和细胞再生过程中的增殖过程 可能涉及多种衰老疾病可能会受到影响，并且 受饮食干预控制。 该提议的另一个目的是 了解CEIR如何控制前态和恶性增生 与某些原始癌基因的表达相关。 这些 调查可能成为延长生命和努力的核心问题 通过饮食干预，人类健康。