AUTOMATION OF STEPS IN LARGE SCALE DNA SEQUENCING

大规模 DNA 测序步骤的自动化

• 批准号: 2208313
• 负责人: Deirdre R. Meldrum
• 金额: $ 11.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2208313
• 关键词: bioengineering /biomedical engineering; biomedical automation; biomedical equipment development; biotechnology; method development; nucleic acid sequence; robotics

The overall objective of this research career award is to develop new methods and tools for automated large-scale DNA sequencing. Initially, the specific aims of the award are to: Train extensively in the field of genomics via lab rotations, courses and seminars in the Department of Molecular Biotechnology. In the lab training the candidate will perform all steps of DNA sequencing via current methods. This experience combined with the candidate's electrical engineering background will be used to develop fast, efficient methods to automate certain steps in large-scale DNA sequencing procedures. Develop a prototype robot that will dispense sub-microliter to sub- nanoliter volumes of reagents for DNA sequencing reactions. A precise robotic positioning system will be designed and built to access individual wells on 384-well or 864-well microtiter plates and alternate denser formats. Investigate and develop a new, automated procedure for loading sequencing reactions onto gels from high density formats. Currently this step in large-scale DNA sequencing is performed manually but with higher density reactions this will be more problematic. Explore alternate electrophoretic formats that require no net migration. Signal processing and identification techniques will be employed to identify DNA molecules in free solution. While performing the steps of large-scale DNA sequencing in lab training during the first year of the five year SERCA program, bottlenecks in large-scale DNA sequencing procedures will be identified. The remainder of the award will be used to focus on solving these problems using new, automated techniques. Ultimately, these technological developments will increase the throughput in DNA sequencing and contribute to the sequencing of the Human Genome. Deirdre Meldrum, the candidate, received her Ph.D. in Electrical Engineering from Stanford University in 1992. At Stanford and the Jet Propulsion Laboratory, Deirdre worked extensively on robotic and controls for space structures, space robots, and computer disk drives. Her strong background in robotics, controls, dynamics, and electrical/mechanical design will be invaluable for fulfilling the goal of automating steps in large-scale DNA sequencing. Throughout the award period and beyond, Deirdre will train and work closely with the faculty in the Department of Molecular Biology while maintaining close ties with the Department of Electrical Engineering. Leroy Hood, the principle faculty advisor, is Professor and Chair of the Department of Molecular Biotechnology at the University of Washington.

该研究职业奖的总体目标是开发新的 自动化大规模 DNA 测序的方法和工具。 最初， 该奖项的具体目标是： 通过实验室轮换、课程和培训，在基因组学领域进行广泛的培训 分子生物技术系的研讨会。 在实验室 培训候选人将通过以下方式执行 DNA 测序的所有步骤 目前的方法。 这种经验结合候选人的 将利用电气工程背景快速、高效地开发 自动化大规模 DNA 测序中某些步骤的方法 程序。 开发一个原型机器人，可以将亚微升的液体分配到亚微升的液体中。 用于 DNA 测序反应的纳升体积试剂。 一个精确的 将设计和建造机器人定位系统以访问 384 孔或 864 孔微量滴定板上的各个孔以及 替代更密集的格式。 研究并开发一种新的自动化上样测序程序 从高密度形式反应到凝胶上。 目前这一步在 大规模 DNA 测序是手动进行的，但密度更高 反应这会更成问题。 探索不需要净迁移的替代电泳格式。 将采用信号处理和识别技术 识别游离溶液中的 DNA 分子。 在实验室培训中执行大规模 DNA 测序步骤时 在 SERCA 五年计划的第一年， 将确定大规模 DNA 测序程序。 剩下的 该奖项的一部分将用于重点使用新的、 自动化技术。 最终，这些技术的发展将 提高 DNA 测序的通量并有助于 人类基因组测序。 候选人迪尔德丽·梅尔德鲁姆 (Deirdre Meldrum) 获得了博士学位。在电气方面 1992 年获得斯坦福大学工程学学位。在斯坦福大学和喷气机公司 迪尔德丽推进实验室在机器人和控制方面进行了广泛的研究 用于空间结构、空间机器人和计算机磁盘驱动器。 她的坚强 机器人、控制、动力学和电气/机械背景 设计对于实现自动化步骤的目标将是无价的 大规模 DNA 测序。 在整个颁奖期间及之后， 迪尔德丽将与该系的教职人员进行培训并密切合作 分子生物学系，同时与该系保持密切联系 电气工程。 勒罗伊·胡德（Leroy Hood），首席教务顾问， 分子生物技术系教授兼系主任 华盛顿大学。