A Comprehensive 5K Plus Glycan Microarray

综合 5K Plus 聚糖微阵列

基本信息

批准号：
10557232
负责人：
Lei Li
金额：
$ 45.4万
依托单位：
Z BIOTECH, LLC
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10557232
关键词：
Address Affinity Avidity Binding Biological Biological Markers Biotechnology Body Fluids Businesses Cell surface Cells Characteristics Collection Complex Computer software Custom Data Analyses Data Scientist Data Set Databases Development Disease Engineering Enzymes Event Financial cost Fostering Funding Glycobiology Glycopeptides Grant Image Immune system Immunotherapy Industrialization Intuition Investments Lectin Libraries Mannose Marketing Mediating Methods Mind Mucins Natural Source Pharmacologic Substance Phase Physiological Polysaccharides Positioning Attribute Process Production Proteins Provider Public Health Quality Control Research Research Institute Research Personnel Resources Route Scientist Signal Transduction Small Business Innovation Research Grant Specific qualifier value Specificity Spottings Structure Sulfate Surface Systems Integration Therapeutic Time Universities Vaccines Vendor analysis pipeline automated analysis commercial application comparative cost experience insight macromolecule manufacturing cost manufacturing scale-up molecular recognition new technology programs receptor research and development scale up sugar nucleotide surface coating tool

项目摘要

Project Summary / Abstract The Glycan Array has proven a valuable tool in the study of glycans. Comparatively the Glycan Array gives the greatest view of glycan binding specificity for the lowest cost in terms of analytical turn-around time and financial cost. The Glycan Array is far from well optimized in our mind. There are three key limitations to an array-based approach with high throughput glycan-protein interaction profiling: 1) glycan arrays suffer from a systematic lack of sensitivity, 2) current array providers lack comprehensive glycan libraries with scalable synthetic routes, and 3) physiological glycan binding is intricate and complex and requires a lengthy analysis process. In Phase I we sought out to find solutions for each of these limitations. First, we developed a first of its kind Glycan Array that can analyze glycan binding avidity not just affinity. Second, we partnered with an elite group of chemists to produce a glycan library containing ~900 glycan structures, which is larger than that of the CFG and one of the largest ever. Third, we partnered with a group of data scientists and bioinformaticians to create a robust software package that automates the entire array data analysis process. Over the last three years we have formed valuable relationships with researchers and clinicians in research institutes, pharmaceutical companies and public health sectors. Z Biotech is perfectly positioned to facilitate the supply for increasing demand for biomedically relevant glycan arrays. In each of these endeavors Z Biotech sincerely thanks to the support of this SBIR grant. Now in Phase II we are seeking funds to continue to find solutions for these key limitations in Glycan Array. First, we will continue to invest heavily in R&D to ensure that our arrays offer superior insights compared to our competitors. We will also use funds from Phase II to scale up production to accommodate increasing demand. Second, we will team with Dr. Lei Li’s group at Georgia State University. Dr. Li is an outstanding glyco-scientist with experience in production of complex glycans, sugar nucleotides and glycan-related enzymes. With his effort we will maintain our current glycan library and add ~2,000 new glycan structures in Phase II. Third, we will complete Z Biotech’s custom software package. In Phase II we will bring this first of its kind glycan array data analysis package or solution to market.

项目摘要 /摘要聚糖阵列已证明是对聚糖研究的宝贵工具。相对而言，聚糖阵列给出了在分析转折时间和财务方面，最低成本的聚糖约束特异性的最大视图成本。在我们脑海中，聚糖阵列远非如此。基于数组有三个关键限制高吞吐量聚糖 - 蛋白质相互作用分析的方法：1）聚糖阵列有系统的缺乏敏感性，2）当前阵列提供商缺乏可扩展合成路线的全面聚糖库，并且 3）生理聚糖结合是复杂而复杂的，需要冗长的分析过程。在第一阶段我们寻求寻找这些限制的解决方案。首先，我们开发了一个同类的聚糖阵列可以分析聚糖结合的亲和力。其次，我们与一群精英化学家合作生产一个包含〜900个聚糖结构的聚糖库，该结构大于CFG和其中一个。有史以来最大。第三，我们与一群数据科学家和生物信息学家合作，创建了一个强大的软件包装可自动化整个数组数据分析过程。在过去的三年中，我们成立了与研究机构，制药公司和研究人员和临床医生的有价值的关系公共卫生部门。 Z Biotech非常适合促进对不断增长的需求的供应生物医学相关的聚糖阵列。在这些努力中，Z BioTech衷心感谢您的SBIR赠款的支持。现在在第二阶段我们是寻求资金继续为聚糖阵列中的这些关键限制找到解决方案。首先，我们将继续大量投资于研发，以确保与竞争对手相比，我们的阵列提供优越的见解。我们也会使用II期的资金来扩大生产，以适应不断增长的需求。其次，我们将与 Lei Li博士在佐治亚州立大学的小组。 Li博士是一位杰出的Glyco-Scientist，具有生产复杂的聚糖，糖核苷酸和与聚糖相关的酶。在他的努力下，我们将维持我们当前的聚糖库，并在第二阶段增加了约2,000个新的聚糖结构。第三，我们将完成Z Biotech的自定义软件包。在第二阶段中，我们将将此类同类阵列阵列数据分析软件包或解决方案。