The Human Lung BioMolecular Multi-Scale Atlas Program (HuBMAP-Lung)

人肺生物分子多尺度图谱计划 (HuBMAP-Lung)

• 批准号: 10530972
• 负责人: GLORIA S PRYHUBER
• 金额: $ 225万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10530972
• 关键词: 3-Dimensional; Adult; Alveolus; Anatomy; Atlases; Biological Assay; Biology; California; Cell Nucleus; Cell physiology; Cells; Chromatin; Collaborations; Collection; Communication; Communities; Coordination and Collaboration; Data; Data Analyses; Data Collection; Disease; Education; Educational workshop; Elements; Experimental Designs; FAIR principles; Gene Expression; Goals; Human; Human BioMolecular Atlas Program; Image; Institution; International; Knowledge; Laboratories; Leadership; Learning; Lipids; Location; Lung; Lung diseases; Maps; Microscopy; Molecular; Names; Nomenclature; North Carolina; Organ; Pacific Northwest; Phase; Pilot Projects; Post-Translational Protein Processing; Production; Productivity; Proteins; Protocols documentation; Public Health; Recording of previous events; Research; Research Personnel; Resolution; Resource Sharing; Resources; Respiratory System; Sensitivity and Specificity; Standardization; Structure; Structure of parenchyma of lung; Technology; Therapeutic Intervention; Trachea; United States National Institutes of Health; Universities; Visualization; Washington; body system; cell type; clinical care; collaboratory; data management; data portal; data sharing; expectation; experience; improved; indexing; interest; interoperability; lung injury; meetings; member; multi-scale atlas; new technology; novel strategies; programs; pulmonary function; repaired; success; tissue mapping; tissue resource; transcriptome sequencing; transcriptomics; working group

Project Summary/Abstract Overall Research Plan The Human Lung Biomolecular Multi-Scale Atlas Program (HuBMAP-Lung) The Human Biomolecular Atlas Program (HuBMAP) is entering the production phase, with an emphasis on progressive, high sensitivity and specificity assays, prioritizing spatial biomolecular analysis. Our Tissue Mapping Center (TMC) proposal will focus on one organ system, comprised of two lungs and related respiratory tract (trachea to alveoli) that for simplicity we refer to as the “Lung”. Our TMC will generate, standardize, and validate extensive data from high content, high-throughput imaging and `omics technology to produce systematic human lung tissue maps at high resolution. We will take a long view of an Atlas, as a collection of 2D and 3D maps containing images, tabular data, facts about multiple locations at varying resolution, and indexes of named objects keyed to coordinates of a locational grid analogous to latitude and longitude, to cartographically present the whole range of salient features of the human Lung. To accomplish this task, our center brings together Investigators from five institutions, the University of Rochester (URMC), University of California at San Diego (UCSD), Pacific Northwest National Laboratory (PNNL), University of Washington (UW), and University of North Carolina at Chapel Hill (UNC), each with complementary lung-focused academic interests, knowledge, and inter- consortia network connections and with internationally recognized expertise in lung and state-of-the-art biomolecular technologies. Our Lung data provided to the HuBMAP Data Portal in Phase I has been focused to single nucleus RNA-sequencing, chromatin availability and beginning spatial transcriptomics. In addition to further increasing representation of human diversity in these data types, the next phase of our TMC turns primary effort toward determining spatial organization of cells and matrices defined by not only gene expression but also proteins, protein modifications, lipids and select metabolites critical to specific cell function within anatomical and functional niches. With the recognized value of global investigative efforts within and outside the Consortium, the Specific Aims of the TMC Overall Component concentrate on Communication and Collaboration within the TMC, between all HuBMAP components and synergistically among national and international researchers and Consortia.

项目摘要/摘要 总体研究计划 人肺生物分子多尺度图集计划（Hubmap-ung） 人类生物分子图集计划（Hubmap）正在进入生产阶段，重点是 进行性，高灵敏度和特异性测定，优先考虑空间生物分子分析。我们的组织映射 中心（TMC）建议将集中在一个器官系统上，由两个肺和相关呼吸道组成 （到达肺泡的气管）为简单起见，我们称为“肺”。我们的TMC将生成，标准化和验证 来自高素质，高通量成像和```OMICS''的广泛数据来产生系统的人类 高分辨率的肺组织图。我们将长时间了解Atlas，作为2D和3D地图的集合 包含图像，表格数据，关于不同分辨率以多个位置的事实以及命名的索引 对象构成类似于纬度和经度的位置网格的坐标，以制图呈现 人类肺的显着特征范围。为了完成这项任务，我们的中心汇集了 加利福尼亚大学圣地亚哥分校罗切斯特大学（URMC）的五个机构的调查人员 （UCSD），太平洋西北国家实验室（PNNL），华盛顿大学（UW）和北大学 卡罗来纳州教堂山（UNC）的卡罗来纳州，每个人都有完全以肺为中心的学术兴趣，知识和间 财团网络连接以及国际认可的肺部和最先进的专业知识 生物分子技术。我们在第一阶段提供给Hubmap数据门户的肺数据已集中在 单核RNA测序，染色质的可用性和开始空间转录组学。此外 在这些数据类型中，进一步增加了人类多样性的表示，我们TMC的下一阶段转向主要 努力确定不仅由基因表达而定义的细胞和物质的空间组织，而且还 蛋白质，蛋白质修饰，脂质和精选的代谢物在解剖学和 功能性壁ni。随着财团内外全球调查工作的公认价值， TMC总体组件集中于沟通和协作的具体目的 TMC，在国家和国际研究人员中，所有Hubmap组件与协同之间 财团。