SBIR PHASE II, TOPIC 019: ELECTROSPUN BIODEGRADABLE MEMBRANE INSERTS

SBIR 第二阶段，主题 019：电纺生物可降解膜插入物

• 批准号: 10505601
• 负责人: KRISTIN TATON
• 金额: $ 197.22万
• 依托单位: INNOVATIVE SURFACE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-24 至 2023-09-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10505601
• 关键词: 3-Dimensional; Adverse effects; Biocompatible Materials; Biological; Biomimetics; Cell Culture Techniques; Cell Line; Cells; Coculture Techniques; Cues; Dimensions; Electrospinning; Environment; Equipment; Extracellular Matrix; Fluorescein-5-isothiocyanate; Growth; Hippocampus (Brain); Image; Imaging Techniques; In Vitro; Kinetics; Life; Measurement; Mechanics; Medical; Membrane; Metabolic; Modeling; Neuroglia; Neurons; Noise; Phase; Property; Rattus; Reproducibility; Research; Research Personnel; Signal Transduction; Small Business Innovation Research Grant; Sterilization; Structure; System; Testing; Thick; Tight Junctions; Tissue Model; Tissues; Work; confocal imaging; cost estimate; cytotoxicity; design; in vitro Assay; in vivo; nanofiber; nanofibrillar; novel; prototype; research study; tissue culture; tool

Cell culture tools expand our abilities to conduct research from studying basic properties to developing specific medical treatments. These tools work best when they mimic cell’s and tissue's biological environment as closely as possible while maintaining high reproducibility in high throughput in vitro assays. As such, cell culture is moving to 3D tissue models, focusing on their in vivo-like properties as opposed to earlier 2D culture. Biodegradable nanofiber membranes offer an advance for in vitro tissue modeling: they contain biomimetic ECM topography, which provides temporary structural support and relevant mechanical and spatial growth cues for nascent tissues. The proposed cell culture biodegradable insert membrane will provide early biomimetic support for seeded cells; cellular extracellular matrix will then replace the function of the membrane as is degrades, resulting in free-standing tissue attached to the insert, for experimentation. To this end, biodegradable membranes will be incorporated into a novel cell culture insert for 96-well plates. Electrospinning will be used to create the nanofibrillar membranes composed of PCL and PLGA, two well characterized biodegradable biomaterials. Two designs will be investigated initially with monoculture cell sheets, and then subsequently, in the Phase I neuronal co-culture model to validate the insert. The insert is required to have specific dimensions, be compatible with imaging techniques used by most researchers, and to have specific pore size and degradation kinetics to mimic integration into the body.

细胞培养工具扩展了我们进行研究的能力，从研究基本特性到开发特定的医学治疗方法，这些工具在尽可能接近地模拟细胞和组织的生物环境，同时在高通量体外测定中保持高再现性时效果最佳。培养正在转向 3D 组织模型，与早期的 2D 培养相比，重点关注其类似体内的特性，这为体外组织建模提供了进步：它们包含仿生 ECM 拓扑结构，可提供类似的特性。新生组织的临时结构支撑和相关的机械和空间生长线索，所提出的细胞培养生物可降解插入膜将为种子细胞提供早期仿生支撑；然后，细胞外基质将取代膜的降解功能，从而形成独立的膜。为此，可生物降解的膜将被纳入 96 孔板的新型细胞培养插入物中，用于制造由以下物质组成的纳米纤丝膜。 PCL 和 PLGA 是两种经过充分表征的生物可降解生物材料，这两种设计将首先使用单一培养细胞片进行研究，然后在第一阶段神经共培养模型中验证插入物需要具有特定的尺寸和兼容性。大多数研究人员使用的成像技术，并具有特定的孔径和降解动力学来模拟融入体内。