Tungsten Biocatalysis - Heavy Metal Enzymes for Sustainable Industrial Biocatalysis

钨生物催化 - 用于可持续工业生物催化的重金属酶

• 批准号: 10097682
• 金额: $ 10.7万
• 依托单位: HYDREGEN LIMITED
• 依托单位国家: 英国
• 项目类别: EU-Funded
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10097682
• 关键词: Tungsten; Biocatalysis; Heavy; Metal; Enzymes

Europe needs a sustainable chemical industry which will only be realized by new breakthrough technologies. Industrial biotechnology is established in chemical manufacturing, offering more efficient, more specific, safer and less energy demanding production, but is held back by the limited number of enzyme classes in industrial use. This project opens up an important new enzyme class of tungstencontaining enzymes (W-enzymes) which catalyse amazing chemical reactions involving challenging low redox potential reduction reactions, but are currently impossible to obtain economically and on scale to match industrial needs. We need to produce W-enzymes using an industrial workhorse micro-organism such as E. coli. Yet, we discovered that W-cofactor biosynthesisisthe bottleneck preventing successful production of W-enzymes in E. coli. We can solve this challenge by using cutting-edge computational enzyme design approaches we recently developed, to create a completely new W-cofactor biosynthesis pathway for E. coli. The W-BioCat strains developed in this project will enable expression of new W-enzymes from genetic databases, and facilitate production of new engineered W-enzymes. The catalytic potential of these new W-enzymes will be established and implemented in new processes. Exciting new reaction scope in biocatalytic CO2 reduction to valuable chemicals and Birch reduction of aromatic compounds will be explored, alongside the already-established and broadly applicable carboxylic acid reductions. W-BioCat will be the breakthrough to make W-enzymes accessible for industry. As a proof of concept, a hydrogen-driven processto convert plant-derived oleic acid to the emollient ester oleyl oleate will be created. Oleyl oleate is used in many cosmetic products used daily by millions of people. This process will be demonstrated in multi-gram yield in scalable, industrially-relevant hydrogenation reactors, together with market research to address a pathway to commercialisation.

欧洲需要一个可持续的化学工业，只能通过新的突破技术实现。工业生物技术是在化学制造中建立的，提供了更高效，更具体，更安全，更少的能源需求生产，但由于工业用途的酶类别有限而阻碍。该项目打开了一种重要的新酶类别的钨含酶（W-酶），该酶催化了惊人的化学反应，涉及挑战低氧化还原潜在的降低反应，但目前不可能在经济上和规模上获得适合工业需求的规模。我们需要使用诸如大肠杆菌之类的工业锻炼微生物生产W-酶。然而，我们发现W-COFACTOR生物合成性瓶颈瓶颈，阻止了大肠杆菌成功产生W-酶。我们可以通过使用最近开发的尖端计算酶设计方法来解决这一挑战，从而为大肠杆菌创建全新的W-Cofactor生物合成途径。该项目中开发的W-BIOCAT菌株将使遗传数据库的新W-酶表达，并促进新工程W-酶的生产。这些新的W-酶的催化潜力将在新过程中建立和实施。将探索已经建立和广泛适用的羧酸降低的芳香化合物的生物催化二氧化碳中令人兴奋的新反应范围。 W-Biocat将是使工业可访问的W-酶的突破。作为概念的证明，将创建以氢驱动的过程将植物来源的油酸转换为润肤剂烯基油酸酯。烯基蛋白油酸盐用于数百万人每天使用的许多化妆品中。该过程将以可扩展的，工业相关的氢化反应堆以及市场研究以解决商业化途径的多克收益率证明。