Nanomaterial Radioisotope Detectors for Intracellular Measurements

用于细胞内测量的纳米材料放射性同位素探测器

• 批准号: 10482292
• 负责人: Colleen Janczak
• 金额: $ 14.98万
• 依托单位: SCINTILLATION NANOTECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10482292
• 关键词: 1-Methyl-4-phenylpyridinium; Area; Beta Particle; Biochemical; Biological; Biological Assay; Biomedical Research; Categories; Cell Line; Cells; Chemicals; Chinese Hamster Ovary Cell; Cimetidine; Cytoplasm; Detection; Development; Disease; Dose; Evaluation; Future; Gold; Government; Hybrids; Industrialization; Innovation Corps; Interview; Investigation; Knowledge; Label; Laboratory Research; Lead; Legal patent; Liquid substance; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Microscopy; Modification; Molecular; Molecular Analysis; Monitor; Nanotechnology; Optics; Organic Cation Transporter; Organic solvent product; POU2F2 gene; Pathway interactions; Pharmacology; Phase; Physiological; Play; Positioning Attribute; Prevalence; Property; Radioisotopes; Radiolabeled; Radiometry; Research; Research Project Grants; Role; Sampling; Scintillation Counter; Scintillation Counting; Seeds; Series; Signal Transduction; Small Business Innovation Research Grant; Specificity; Structure; Surface; Technology; Time; Tissues; Toxic effect; Tracer; Water; Work; absorption; aqueous; base; biological systems; detector; drug discovery; human disease; improved; innovation; nanomaterials; nanoparticle; nanoscale; programs; public health relevance; research clinical testing; sensor; small molecule; surfactant; temporal measurement; uptake

The capability to analyze cellular uptake of physiological and pharmacological compounds with increasing sensitivity and temporal resolution plays a critical role in understanding the underlying molecular pathways involved in human diseases and disorders. Among the most challenging analytes are those present at very low concentrations with high temporal variability, and small molecules lacking moieties amenable for optical and/or electrochemical detection. Radioisotopes (RI) facilitate highly sensitive detection of these analytes, with minimal perturbation of analyte mass and structure, compared to fluorescent labels and other molecular tags. β-particle emitters, including 32P, 33P, 35S, and 3H are commonly used as biological tracers due to the prevalence of these atoms in biological molecules. Despite the advent of new molecular analysis approaches, RI remain the gold standard in a wide range of quantitative biological, chemical and environmental studies. RI labels have played a critical role in the investigation of biological systems, for almost a century. Though new analytical approaches, including mass spectrometry, now allow sensitive detection of label-free mixtures, the high mass sensitivity of radioassays is unparalleled, making radioassays indispensable for a range of biochemical and biomedical studies. Furthermore, improved detection capabilities that enable a broader application of RI detection to new biomedical research questions should prove transformational in high sensitivity biomolecular analyses. In this SBIR application, we propose to continue development of proprietary, IP-protected, innovative core-shell nanomaterials capable of real-time, and in some cases, molecularly selective, detection of low energy RIs directly in aqueous samples. These nanomaterials be demonstrated for intracellular measurement of low energy RI- labeled analytes. Such measurements would seed new research investigations and would position Scintillation Nanotechnologies INC as the only commercial entity providing products that perform such measurements.

分析物理和药物化合物的细胞摄取的能力随着增加而增加 灵敏度和临时分辨率在理解潜在分子途径中起着关键作用 参与人类疾病和疾病。最挑战的分析物是非常低的人 具有高临时变异性的浓度和缺乏光学和/或可容纳的小分子 电化学检测。放射性同位素（RI）对这些分析物的高度敏感检测，最少 与荧光标签和其他分子标签相比，分析物质量和结构的扰动。 β粒子 由于这些流行率，包括32p，33p，35s和3H在内 生物分子中的原子。 尽管有新的分子分析方法冒险，但RI仍然是广泛的金标准 定量生物学，化学和环境研究。 RI标签在 对生物系统的调查已有近一个世纪。尽管新的分析方法，包括质量 光谱法，现在允许对无标签混合物的敏感检测，无射线标记的高质量灵敏度为 无与伦比的，使得针对一系列生化和生物医学研究必不可少。此外， 改进的检测功能，可以在新的生物医学研究中更广泛地应用RI检测 在高灵敏度生物分子分析中，问题应证明是转化的。 在此SBIR应用程序中，我们建议继续开发专有的，受IP的创新核心壳 纳米材料能够实时，在某些情况下是分子选择性的，直接检测低能量的RI 在水性样品中。这些纳米材料被证明用于细胞内测量低能量RI- 标记的分析物。这样的测量将使新的研究调查播种，并将闪烁定位 纳米技术公司是唯一提供执行此类测量产品的商业实体。