PHS2019-02 Omnibus Solic of the NIH, CDC, and FDA for SBIR Apps No Clinical Trial (Parent SBIR R43/4

PHS2019-02 NIH、CDC 和 FDA 的 SBIR 应用程序综合 Solic 尚未进行临床试验（母公司 SBIR R43/4

• 批准号: 10476977
• 负责人: STEVEN A BENNER
• 金额: $ 48.52万
• 依托单位: FIREBIRD BIOMOLECULAR SCIENCES, LLC
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10476977
• 关键词: PHS2019; 02; Omnibus; Solic; NIH

Field Detection of arboviruses directly from mosquito traps Firebird Biomolecular Sciences, LLC. Abstract Mosquitoes are the world's deadliest creatures. In the US, mosquitoes are vectors for West Nile, many kinds of encephalitis, and viruses arriving Latin America and Africa. In the developing world, they cause millions of cases of disease and death each year. Routine insect spraying is not a "fix", as it leads to pesticide resistance, encounters public opposition, and destroys beneficial insects. Thus, the EPA, CDC, ECDPC, and their counterparts around the globe, strongly recommend surveillance before spraying. Florida law requires it. In Phase 1, we created (a) innovative technology to generate CO2 to attract mosquitoes, (b) innovative tech- nology to collect viral RNA from the attracted mosquitoes, (c) data showing that collected RNA is stable under Florida summer conditions for at least 3 days, (d) processes where captured viral RNA is amplified directly without handling mosquitos or Qiagen RNA preps, and (e) reagents that allow robust multiplex amplification. These specs, which can transform the science and practice of arbovirus surveillance, attracted TrakITNow, an innovator in instrument design. Its award-winning trap (Moskeet) counts and speciates mosquitoes, doing everything short of collecting samples from the mosquitoes and determining if these hold arboviruses. This Phase 2 project will re-configure and metric the Phase 1 innovations to allow them, in Phase 3, to be placed on Moskeet in two operational architectures (OA1 and OA2). A series of improvement and adaptations will be evaluated using tightly crafted metrics in two Aims. Meeting these metrica will trigger a commitment by TrakITNow to support Phase 3 funding to launch a combined product, Aim 1 will develop and metric Firebird's chemistry for an OA1 architecture that will convert Moskeet from a trap-count-speciate-communicate product into a trap-count-speciate-sample-communicate product. This will exploit Firebird's Phase 1 innovation (SterileQTM) that autonomously traps and stabilizes viral RNA. Different baits, RNA-stabilizing preservatives, and pre-amplification enzymes will be metricked to create, at the end of Year 1, a Moskeet-ready OA1 chemistry that allows continuous sampling of mosquitoes. Metrics include (a) >50% of infected mosquitoes leaving detectable sample, (b) >200 hours half-life of RNA, and (c) capture of >1 pg of RNA. An instrument from Insilixa (Hydra), also enhanced by Firebird's reagents, will be used for on-site virus detection. Hydra is portable, and its low complexity design allows it to be used by amateurs. Aim 2 will develop and metric Firebird's chemistry for an OA2 architecture. This will convert Moskeet into a trap-count-speciate-sample-analyze-communicate product. Here, we will develop and benchmark point-of- sampling amplification tools, specifically: (a) RT-PCR, and (b) loop amplification (LAMP). These will, as needed, incorporate reagent innovations (AEGIS, SAMRS, Biversals) to support highly multiplexed detection of arboviruses, create uniform amplification of multiple analytes, manage primer-primer products, prevent loss of PCR resources to off-target processes that create "monsters", and manage arbovirus sequence divergence.

直接从蚊子陷阱的弧菌病毒的现场检测 火鸟生物分子科学有限责任公司。 抽象的 蚊子是世界上最致命的生物。在美国，蚊子是西尼罗河的向量，许多种类 脑炎以及到达拉丁美洲和非洲的病毒。在发展中国家，它们造成了数百万 疾病和死亡病例每年。常规的昆虫喷涂不是“修复”，因为它会导致农药耐药性， 遇到公众反对，并破坏有益的昆虫。因此，EPA，CDC，ECDPC及其 在全球范围内，强烈建议在喷洒之前进行监视。佛罗里达州法律要求这一点。 在第1阶段，我们创建了（a）创新技术来生成二氧化碳来吸引蚊子，（b）创新的技术 - 从吸引的蚊子收集病毒RNA的一种方法，（c）数据，显示收集的RNA在下面是稳定的 佛罗里达州夏季条件至少3天，（d）捕获的病毒RNA的过程直接放大 不处理蚊子或Qiagen RNA的准备，以及（E）允许多重扩增的试剂。 这些规格可以改变Arbovirus监视的科学和实践，吸引了Trakitnow，这是一个 仪器设计中的创新者。其屡获殊荣的陷阱（Moskeet）计数并规定了蚊子 除了从蚊子中收集样品并确定这些是否持有弧病毒的一切都没有。 该第2阶段项目将重新配置并度量1阶段创新，以使其在第3阶段中成为 将两个操作架构（OA1和OA2）放在Moskeet上。一系列改进和改编 将在两个目标中使用紧密制作的指标进行评估。满足这些Metrica将引发一项承诺 Trakitnow支持第三阶段资金推出合并产品， AIM 1将开发和公制的Firebird的OA1体系结构的化学反应 将陷阱分布式交流的产品陷入陷阱量表类样品群中。这会 利用Firebird的第1阶段创新（Interileqtm）自主捕获并稳定病毒RNA。不同的 诱饵，稳定的防腐剂和预扩增酶将在结束时创建 1年级，一种可摩擦的OA1化学，可以连续采样蚊子。指标包括（a） > 50％的感染蚊子留下可检测的样品，（b）> 200小时的RNA半衰期，（c）捕获> 1 RNA的Pg。 Insilixa（Hydra）的一种仪器（也可以通过火鸟的试剂增强）将用于现场 病毒检测。九头蛇是便携式的，其低复杂性设计允许业余爱好者使用它。 AIM 2将开发OA2建筑的公制火鸟的化学。这会将moskeet转换为 陷阱量表样品 - 分析式共传输产品。在这里，我们将开发和基准测试 特别是采样放大工具：（a）RT-PCR和（b）循环放大（LAMP）。这些将是 需要，将试剂创新（宙斯盾，萨姆斯，载火）合并，以支持对高度多重检测 arboviruses，创建多个分析物的统一扩增，管理引物制品产品，防止损失 PCR资源可用于创建“怪物”并管理Arbovirus序列差异的目标流程。