Micro-CT Imaging Core

Micro-CT 成像核心

• 批准号: 10475073
• 负责人: Xiaowei Sherry Liu
• 金额: $ 13.39万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10475073
• 关键词: 3-Dimensional; Acute; Aging; Animal Model; Animals; Biological Assay; Biological Process; Cartilage; Chronic; Clinical Research; Collaborations; Communities; Complement; Computer software; Consultations; Custom; Data; Data Analyses; Development; Disease; Doctor of Philosophy; Evaluation; Event; Faculty; Fibrocartilages; Floor; Foundations; Freezing; Funding; Genetic; Glycosaminoglycans; Gold; Health; Histologic; Histological Techniques; Histology; Home; Human; Image; Imaging Techniques; Immunohistochemistry; Intervertebral disc structure; Laboratories; Laboratory Animals; Location; Maintenance; Measurement; Mechanics; Methods; Minerals; Modality; Modeling; Morphology; Muscle; Musculoskeletal; Musculoskeletal Development; Musculoskeletal Diseases; Musculoskeletal Physiology; Musculoskeletal System; Paraffin; Pathologic; Pennsylvania; Peripheral; Philadelphia; Process; Research; Research Design; Research Personnel; Resolution; Resources; Roentgen Rays; Services; Skeleton; Stains; Structure; Techniques; Tendon structure; Time; Tissue Engineering; Tissue Model; Tissue Sample; Tissues; Training; University resources; X-Ray Computed Tomography; base; bone; bone imaging; calcification; clinical imaging; contrast enhanced; density; extracellular; human tissue; imaging approach; imaging modality; in vivo; injury and repair; innovation; insight; member; microCT; novel; programs; protocol development; repaired; service delivery; soft tissue; three-dimensional visualization; tool

The mechanical and biological functions of musculoskeletal tissues are based on their structure, organization and composition. Maintenance of tissue integrity, acute changes with injury and repair, as well as progressive and chronic changes with aging and disease, can be evaluated and quantified on the sub-cellular, cellular, extracellular, and tissue level by histological techniques. Normal and pathological human tissues and animal models are widely used to study the cellular, extracellular, and tissue determinants of these processes. Thus, qualitative and analytical descriptions of key histological changes by standard and advanced staining techniques, and assessment and measurements of important histomorphological parameters in human tissue and animal models are at the foundation of examining critical questions in musculoskeletal health and disease. The overall objective of this Histology Core is to develop and apply a wide range of standard and innovative histological and histomorphometric approaches to evaluate musculoskeletal tissue structure and composition, and to provide training and funding for new projects and collaborations using these approaches. Due to their heterogeneous structures and compositions, musculoskeletal tissues present unique experimental and analytical challenges that require customized, sensitive and diverse histological assays. The PCMD Histology Core provides sophisticated expertise, and a suite of standard and novel tools and techniques, which are specially tailored for the complete range of musculoskeletal tissues, including intervertebral disc, bone, cartilage, fibrocartilage, tendon and muscle from large and small animal models, human tissue samples, and tissue-engineered constructs. Conventional techniques supported by the core include paraffin, frozen and plastic processing, embedding, sectioning and histochemical staining, as well as imaging and analysis, and more advanced and sophisticated techniques such as immunohistochemistry and histomorphometry. Additional new, cutting edge techniques will be added to the suite of core capabilities in this renewal. The Histology Core also provides educational programs for PCMD members and trainees through a range of mechanisms focusing on standard and advanced histological techniques, and their application to musculoskeletal research across a spectrum of healthy and disease conditions. With respect to service delivery, the core offers both full and self-service options, complemented by study design consultation and data interpretation to maximize the Core’s impact. A popular and fully subsidized protocol development and optimization service is offered to users to envision and jumpstart novel, application-specific histological techniques. Through this renewal, the Histology Core will continue to serve as a unique and indispensable resource for researchers at UPenn and in the wider community, catalyzing innovative and high impact musculoskeletal research, and stimulating collaborations between current and new PCMD members who may have not previously included histological approaches in their musculoskeletal research programs.

肌肉骨骼组织的机械和生物学功能基于其结构， 组织和组成。 可以在亚细胞上评估和量化的进行性和疾病的渐进性变化和疾病的变化 通过组织学技术和病理人体组织，细胞外和组织水平 动物模型被广泛用于研究这些过程的细胞，细胞外和组织决定因素。 因此，通过标准和高级染色对关键组织学变化的定性和分析描述 人类tisue重要的组织形态参数的技术，评估和测量 动物模型是检查关键的探索Qusculoskleoskeletal和疾病的基础。 组织学核心的总体目标是开发和应用各种标准和 创新的组织学，组织学和组织形态计量学方法，用于评估肌肉促进组织 结构和组成，并为新项目和合作提供培训和资金 使用这些方法，由于它们的异质结构和组成 目前需要定制，敏感和多样化的独特实验和分析挑战 组织学分析。 新颖的工具和技术，专门针对整个肌肉骨骼组织范围量身定制 包含椎间盘，骨骼，软骨，纤维球杆菌，肌腱和大型动物的肌腱和肌肉 模型，人体组织样品和组织工程构建体。 核心包括石蜡，冷冻和塑料加工，嵌入，切片和组织化学纹章 作为成像和分析，以及更先进和复杂的技术，例如免疫组织化学 和组织形态计量学。 这种续约。组织学核心还为PCMD成员和受训者提供教育计划 一系列着重于标准和高级组织学技术的机制及其应用 在健康和疾病方面的肌肉骨骼研究。 交付，核心提供了完整和自助服务的选项，并通过研究设计咨询和数据进行了汇编 解释以最大化核心的影响。 优化服务向用户提供给用户，以设想和快速启动小说，特定于应用程序特定的组织学 通过这种续订，组织学核心将继续作为Anique 为Upenn和更广泛社区的研究人员提供资源，催化创新和高影响力 肌肉骨骼研究，并刺激可能 以前没有将组织学方法纳入其肌肉骨骼tearch程序。