Rapid Point-of-Care RNA Diagnostic for Dengue Infection

登革热感染的快速护理点 RNA 诊断

基本信息

批准号：
10469689
负责人：
HyunDae Cho
金额：
$ 99.98万
依托单位：
CROSSLIFE TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-15 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10469689
关键词：
Achievement Antibodies Antigens Area Base Sequence Biological Assay Biosensor Blood Blood Tests Blood specimen Borrelia Brazil Centers for Disease Control and Prevention (U.S.)Cessation of life Chikungunya virus Clinical Colombia Containment Country Cytomegalovirus Dangerousness Data Data Set Dengue Dengue Fever Dengue Infection Dengue Virus Detection Development Devices Diagnostic Diagnostic tests Disease Dissociation Early Diagnosis Epidemic Evaluation Feedback Fever Goals Health Herpesvirus 1 Herpesvirus Type 3 Hospitals Human Human Resources Infection Instruction Laboratories Lateral Latin America Leptospira Malaria Methods Modification Molecular Monitor Nucleic Acid Amplification Tests Nucleic Acids Organism Outcome Measure Pan American Health Organization Panama Paper Patients Performance Periodicity Peripheral Phase Power Sources Preparation Process Public Health Publications Quarantine RNA Rapid diagnostics Reagent Recommendation Refrigeration Reporting Reproducibility Resource-limited setting Resources Reverse Transcriptase Polymerase Chain Reaction Rickettsia Running Sampling Sensitivity and Specificity Singapore Site South America Southeastern Asia Specificity Specimen St. Louis Encephalitis Virus Supportive care System Taiwan Technology Testing Time Training Triage Universities Validation Virus West Nile virus Whole Blood World Health Organization Yellow fever virus antigen test commercialization cost cross reactivity design detection assay detection limit detection method detection test diagnostic assay economic impact expedited review field study innovative technologies instrument instrumentation medical schools mosquito-borne new technology pathogen pathogenic virus performance tests point of care point-of-care diagnostics public health relevance rapid detection rapid test test strip viral RNA waiver

项目摘要

Abstract Rapid Point-of-Care RNA Diagnostic for Dengue Infection CrossLife Technologies Inc. Project Summary/Abstract Dengue viruses (DENV) are one of the most dangerous and significant pathogens in the world, and there are 230 million cases of dengue virus infections per year, mostly in Latin America and Southeast Asia. These mosquito-borne viruses cause periodic explosive epidemics in many tropical and sub-tropical countries. CrossLife technologies' dengue diagnostic is being developed to detect dengue infection early at the point-of-care so that patients can be treated effectively. Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and containment of the disease. However, current methods for early detection of dengue involve the use of RT-PCR instruments in the laboratory, which may not be available in areas where dengue is endemic, and which may be too slow. CrossLife Technologies proposed to develop a Template Assisted Rapid Assay (TARA) diagnostics for detection of dengue viruses in human blood. TARA dengue diagnostic will detect dengue in 30 minutes on a paper strip at the point-of-care (POC) so that patients can be treated rapidly and at low cost. This innovative technology will provide PCR-less, instrumentation-free, low-cost, disposable, rapid and sensitive POC dengue diagnostics directly from whole blood samples.

抽象的登革热感染Crosslife Technologies Inc.的快速护理RNA诊断项目摘要/摘要登革热病毒（DENV）是世界上最危险和最重要的病原体之一，每年有2.3亿例登革热病毒感染病例，大多是在拉丁美洲和东南亚。这些蚊子传播的病毒在许多热带和亚热带国家引起周期性爆炸性流行病。正在开发越野技术的登革热诊断，以便在护理的早期检测登革热感染，以便可以有效治疗患者。在高热期间，早期和快速检测到登革热病毒（DENV）感染对于适当的患者治疗和疾病的控制至关重要。但是，当前早期发现登革热的方法涉及在实验室中使用RT-PCR仪器，在登革热是地方性的地区可能无法使用，并且可能太慢。提议开发模板辅助快速测定（TARA）诊断的跨生命技术，以检测人类血液中的登革热病毒。塔拉登革热诊断将在30分钟内在造纸地带（POC）的纸条带上检测到登革热，以便可以快速以低成本治疗患者。这种创新的技术将直接从全血样品中直接提供无PCR，无仪器，低成本，可置，快速和敏感的POC登革热诊断。