Rapid Point-of-Care RNA Diagnostic for Dengue Infection

登革热感染的快速护理点 RNA 诊断

基本信息

批准号：
10436535
负责人：
HyunDae Cho
金额：
$ 99.98万
依托单位：
CROSSLIFE TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-15 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10436535
关键词：
Achievement Antibodies Antigens Area Base Sequence Biological Assay Biosensor Blood Blood Tests Blood specimen Borrelia Brazil Centers for Disease Control and Prevention (U.S.)Cessation of life Chikungunya virus Clinical Colombia Containment Country Cytomegalovirus Dangerousness Data Data Set Dengue Dengue Fever Dengue Infection Dengue Virus Detection Development Devices Diagnostic Diagnostic tests Disease Dissociation Early Diagnosis Epidemic Evaluation Feedback Fever Goals Health Herpesvirus 1 Herpesvirus Type 3 Hospitals Human Human Resources Infection Instruction Laboratories Lateral Latin America Leptospira Malaria Methods Modification Molecular Monitor Nucleic Acid Amplification Tests Nucleic Acids Organism Outcome Measure Pan American Health Organization Panama Paper Patients Performance Periodicity Peripheral Phase Power Sources Preparation Process Public Health Publications Quarantine RNA Rapid diagnostics Reagent Recommendation Refrigeration Reporting Reproducibility Resources Reverse Transcriptase Polymerase Chain Reaction Rickettsia Running Sampling Sensitivity and Specificity Singapore Site South America Southeastern Asia Specificity Specimen St. Louis Encephalitis Virus Supportive care System Taiwan Technology Testing Time Training Triage Universities Validation Virus West Nile virus Whole Blood World Health Organization Yellow fever virus antigen test commercialization cost cross reactivity design detection assay detection limit detection method detection test diagnostic assay economic impact expedited review field study innovative technologies instrument instrumentation medical schools mosquito-borne new technology pathogen pathogenic virus performance tests point of care point-of-care diagnostics public health relevance rapid detection rapid test test strip viral RNA waiver

项目摘要

Abstract Rapid Point-of-Care RNA Diagnostic for Dengue Infection CrossLife Technologies Inc. Project Summary/Abstract Dengue viruses (DENV) are one of the most dangerous and significant pathogens in the world, and there are 230 million cases of dengue virus infections per year, mostly in Latin America and Southeast Asia. These mosquito-borne viruses cause periodic explosive epidemics in many tropical and sub-tropical countries. CrossLife technologies' dengue diagnostic is being developed to detect dengue infection early at the point-of-care so that patients can be treated effectively. Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and containment of the disease. However, current methods for early detection of dengue involve the use of RT-PCR instruments in the laboratory, which may not be available in areas where dengue is endemic, and which may be too slow. CrossLife Technologies proposed to develop a Template Assisted Rapid Assay (TARA) diagnostics for detection of dengue viruses in human blood. TARA dengue diagnostic will detect dengue in 30 minutes on a paper strip at the point-of-care (POC) so that patients can be treated rapidly and at low cost. This innovative technology will provide PCR-less, instrumentation-free, low-cost, disposable, rapid and sensitive POC dengue diagnostics directly from whole blood samples.

抽象的登革热感染快速护理点 RNA 诊断 CrossLife Technologies Inc. 项目摘要/摘要登革热病毒 (DENV) 是世界上最危险和最重要的病原体之一，每年有 2.3 亿例登革热病毒感染病例，主要分布在拉丁美洲和东南亚。这些蚊媒病毒在许多热带和亚热带国家引起周期性爆发性流行病。 CrossLife Technologies 的登革热诊断技术正在开发中，旨在在护理点及早发现登革热感染，以便患者能够得到有效治疗。在发热期间及早、快速检测登革热病毒 (DENV) 感染对于正确的患者管理和控制疾病至关重要。然而，目前登革热早期检测方法涉及在实验室使用RT-PCR仪器，这在登革热流行地区可能无法使用，而且检测速度可能太慢。 CrossLife Technologies 提议开发一种模板辅助快速检测 (TARA) 诊断方法，用于检测人类血液中的登革热病毒。 TARA 登革热诊断仪将在 30 分钟内在护理点 (POC) 的纸条上检测出登革热，以便患者能够以较低的成本快速得到治疗。这项创新技术将直接从全血样本中提供无 PCR、无需仪器、低成本、一次性、快速且灵敏的 POC 登革热诊断。