Gene Modulation inCancer Cellular Models

癌症细胞模型中的基因调控

• 批准号: 10439813
• 负责人: Jun Xu
• 金额: $ 13.89万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439813
• 关键词: Address; Animal Model; CRISPR/Cas technology; Cancer Center; Cancer Center Support Grant; Cancer Patient; Cancer cell line; Cell Line; Cell model; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collection; Complement; Complementary DNA; Computers and Advanced Instrumentation; Custom; Development; Disease remission; Elements; Epigenetic Process; Gene Proteins; Gene Targeting; Genes; Genetic; Genetic Screening; Genetic Transcription; Genome; Genomic Library; Genomics; Goals; Housing; Human; Human Characteristics; Individual; Investigation; Laboratories; Lentivirus; Libraries; Malignant Neoplasms; Mammalian Cell; Mediating; Medicine; Modeling; Modification; Mutation; Pathway interactions; Patients; Physiological; Preparation; Production; RNA Interference; Research; Research Personnel; Resource Sharing; Resources; Robotics; Services; Somatic Cell; System; Technology; Transfection; Tumor Suppressor Genes; Work; anticancer research; base; cancer cell; cancer heterogeneity; cancer stem cell; cell type; cohesion; college; cost; established cell line; functional genomics; gene function; genome editing; genome-wide; human pluripotent stem cell; improved; induced pluripotent stem cell; member; neoplasm resource; neoplastic cell; new therapeutic target; personalized cancer therapy; personalized therapeutic; protein function; small hairpin RNA; stem cells; technology development; tool; tumor; vector; whole genome

PROJECT SUMMARY: Gene Modulation in Cancer Cell Models (GMCCM) Shared Resource The complexity and heterogeneity of cancer demand appropriate and adaptable cell-based models that enable researchers to uncover key genes, pathways, interactions, and modifications that drive cancer development and to devise effective and personalized therapeutics. The Gene Modulation in Cancer Cell Models (GMCCM) Shared Resource provides DLDCCC members with a versatile combination of cutting-edge technologies, genomic resources, and advanced instrumentation. Directed by Drs. Dan Liu and Jean Kim, who combined have extensive expertise in developing technologies and cancer cell models (e.g., high-throughput genetic screens and stem cell manipulation), GMCCM houses essential elements for single-gene analyses to whole- genome screens, including genome-wide libraries of short-hairpin RNAs (shRNAs), cDNAs, and CRISPR sgRNAs, as well as multiple automated robotic platforms for library manipulation. In addition, the Shared Resource offers CRISPR/Cas9-mediated genome-editing services in a variety of cell types including induced pluripotent stem cells (iPSCs) derived from cancer patients. The ever-expanding functionalities of CRISPR/Cas9 that include gene targeting, genome modification, and transcriptional modulation, promise to greatly benefit DLDCCC members and facilitate both mechanistic and exploratory investigations using cancer cell models. Specific services provided by the GMCCM Shared Resource include cellular reprogramming, large-scale automated manipulation and preparation of genomic libraries, utilizing individual cDNA/shRNA/sgRNA vectors, and automated mammalian cell transfection and lentivirus production in arrayed formats. Importantly, GMCCM assists DLDCCC members with highly customizable gene-editing services in cells of their choice, from primary cells and established cell lines, to human pluripotent stem cells (hPSCs) and cancer patient-derived induced PSCs. By housing these resources in a single, cohesive Shared Resource, GMCCM allows investigators to employ myriad genomic and genetic platforms that are often cost- and labor- prohibitive or simply not feasible for individual laboratories. This synergistic combination of expertise and resources makes GMCCM an invaluable asset to the DLDCCC.

项目摘要：癌细胞模型（GMCCM）共享资源中的基因调节 癌症的复杂性和异质性要求适当且基于适应性的细胞模型，以实现 研究人员发现促进癌症发展的关键基因，途径，相互作用和修饰 并设计有效和个性化的治疗剂。癌细胞模型（GMCCM）中的基因调节 共享资源为DLDCCC成员提供了尖端技术的多功能组合， 基因组资源和高级仪器。由Drs执导。丹·刘（Dan Liu）和让·金（Jean Kim） 在开发技术和癌细胞模型方面拥有广泛的专业知识（例如，高通量遗传 筛选和干细胞操作），GMCCM包含用于单基因分析的重要元素 基因组屏幕，包括全基因组的短发RNA（SHRNA），cDNA和CRISPR的库 SGRNA，以及多个用于图书馆操作的自动化机器人平台。另外，共享 资源提供CRISPR/CAS9介导的基因组编辑服务，包括各种细胞类型 源自癌症患者的多能干细胞（IPSC）。不断扩展的功能 包括基因靶向，基因组修饰和转录调制的CRISPR/CAS9，承诺 极大地使DLDCCC成员受益，并促进使用癌症的机械和探索性研究 细胞模型。 GMCCM共享资源提供的特定服务包括蜂窝重编程， 使用个人的大规模自动操作和基因组库的准备 cDNA/shRNA/sGRNA载体，以及在阵列中的自动化哺乳动物细胞转染和慢病毒的产生 格式。重要的是，GMCCM协助DLDCCC成员提供高度可自定义的基因编辑服务 他们选择的细胞，从主要细胞和已建立的细胞系，到人多能干细胞（HPSC）和 癌症患者衍生的诱导PSC。通过将这些资源载入单个共享资源中， GMCCM允许研究人员采用众多基因组和遗传平台，这些平台通常是成本和劳动力的 对于个别实验室而言，过敏或根本不可行。专业知识和 资源使GMCCM成为DLDCCC的宝贵资产。