Thermo Orbitrap Eclipse Tribrid with ETD and an Ultimate 3000 RSLCnano System

带 ETD 和 Ultimate 3000 RSLCnano 系统的 Thermo Orbitrap Eclipse Tribrid

• 批准号: 10431695
• 负责人: Shouling Xu
• 金额: $ 60万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431695
• 关键词: System

This proposal is seeking funds for a Thermo Orbitrap Eclipse Tribrid with ETD and an Ultimate 3000 RSLCnano system to meet increasing demands for proteomic analysis for 10 labs from two departments of Carnegie Institution (nine are funded by NIH) and for three labs at Stanford University ( two are funded by NIH). The CISMS is equipped with one instrument Orbitrap Q Exactive HF. With only HCD mode and lack of MS3 functionality, lack of sophisticated configuration and software, the current instrument can no longer meet the demands of current NIH-funded research at our institution. In particular, the needs of many researchers in the area of posttranslational modifications (such as O-GlcNAcylation, O-fucosylation, prenylation, methylation), cross-linking mass spectrometry, cell-type specific and single-cell proteomics, rRNA modification, protein-RNA interface mapping, and enhanced quantification using TMT multiplexing, are currently not being met with existing instrumentation. Thus, the planned acquisition of Orbitrap Eclipse ETD is crucial for the development and completion of NIH-supported research, and thereby is fully backed by our research community. The proposed instrument is to be housed within Carnegie Institution for Science Mass Spectrometry facility(CIS-MS) which is highly used by researchers throughout the region. The requested Orbitrap Eclipse with ETD not only doubles the capacity of the facility, but also provides several key capabilities not available on the current instrument of the facility. In particular, ETD and hybrid EThcd fragmentation modes, with MS3 and with sophisticated configuration and software, are essential for projects that study site-specific post-translational modification analysis such as O-GlcNAcylation and O-fucosylation, and prenylation, and structural analysis using MScleavable cross-linker, and rRNA post-transcriptional modifications. The ability to perform real-time search, synchronous precursor selection (SPS) and MS3 fragmentation for TMT isobaric quantification is requested from several groups for projects that demand high accuracy, high sensitivity and high throughput for proteomics and phosphoproteomics, cell type proteomics and single-cell proteomics. Operation and maintenance of the instrument will be performed by experienced staff in CIS-MS. The Carnegie institution provides the $567,739.80 match required to meet the full price of the system, and ensures full support for the facility director and two supporting staff, as well as service contract for year 2 and the remaining effective lifetime of the instrument after the one-year warranty.

该提案正在寻求用ETD和最终3000 RSLCNANO的Thermo Orbitrap Eclipse Tribrid的资金 满足来自卡内基两个部门的10个实验室蛋白质组学分析需求不断增长的系统 机构（九个由NIH资助）和斯坦福大学的三个实验室（两个由NIH资助）。开心 配备了一个仪器Orbitrap Q Extive HF。只有HCD模式和缺乏MS3功能， 缺乏复杂的配置和软件，当前的仪器无法再满足 当前的NIH资助的研究。特别是，许多研究人员在翻译后领域的需求 修饰（例如O-Glcnacylation，O-核苷酸化，原始化，甲基化），交联 质谱，细胞类型特异性和单细胞蛋白质组学，rRNA修饰，蛋白-RNA界面 目前尚未使用现有的映射和​​增强的量化量化 仪器。因此，计划对Orbitrap Eclipse ETD的收购对于开发和 完成了NIH支持的研究，因此得到了我们的研究社区的完全支持。提议 仪器应安置在卡内基科学质谱设施（CIS-MS）中 在整个地区的研究人员高度使用。 ETD的请求的Orbitrap Eclipse不仅双打 该设施的能力，但还提供了当前工具上没有的几种关键功能 设施。特别是，ETD和杂交EthCD碎片模式，具有MS3，并且具有复杂性 配置和软件，对于研究特定网站特定后翻译后修改的项目至关重要 分析，例如使用MSCLEAVED的O-Glcnacylation和O-核苷酸化以及前苯基化和结构分析 交叉链接和rRNA转录后修改。执行实时搜索的能力， 从要求从同步前体选择（SPS）和MS3碎片TMT等同量定量化 几个小组，需要高准确性，高灵敏度和高吞吐量的蛋白质组学和高通量 磷蛋白质组学，细胞类型蛋白质组学和单细胞蛋白质组学。操作和维护 仪器将由CIS-MS的经验丰富的员工执行。卡内基机构提供$ 567,739.80 满足系统的全价所需的比赛，并确保对设施主管和两个 支持人员以及2年级的服务合同以及仪器的剩余寿命之后 一年保修。