Live Cell Imaging System for Biomechanics and Mechanobiology Research

用于生物力学和力学生物学研究的活细胞成像系统

• 批准号: 10431500
• 负责人: Gunes Uzer
• 金额: $ 23.32万
• 依托单位: BOISE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431500
• 关键词: 3-Dimensional; Arts; Basic Science; Biology; Biomechanics; Biomedical Engineering; Biomedical Research; Bioreactors; Cells; Centers of Research Excellence; Coupled; Cues; Custom; Disease; Doctor of Philosophy; Engineering; Equipment; Faculty; Funding; Goals; Growth; Health; Health Sciences; Idaho; Institution; Investigation; Investments; Link; Mechanics; Microscope; Microscopy; Molecular; Musculoskeletal; Musculoskeletal System; Orthopedics; Physiological; Pongidae; Positioning Attribute; Process; Research; Research Personnel; Science; System; Tissues; United States National Institutes of Health; Universities; clinical application; college; extracellular; imaging system; innovation; instrument; live cell imaging; programs; rapid growth

Project Summary The goal of this proposal is to acquire a Zeiss Axio Observer 7 Live Cell Imaging system which will be coupled with available custom-made and commercial bioreactor systems that will enable biomedical research focused on deformation microscopy, mechano-adaptation and cell tracking approaches for researchers at Boise State as well as neighboring and collaborator institutions. Led by NIH initiatives Center of Biomedical Research Excellence (COBRE) at Matrix Biology and Idaho Network of Biomedical Research Excellence (INBRE) there was a rapid growth of biomedical research focused at biomechanics and mechanobiology in Boise State. These efforts are further supported by both new PhD programs like Biomedical Engineering and by established PhD programs such Materials Engineering and Biomolecular Sciences that seen increased Bioengineering focus over the years. Despite this growth, there is only one confocal microscope to serve all the researchers in Boise State. This not only bottlenecks the use of confocal system which is better suited for 3D applications, but also limits mechanistic approaches to study how extracellular cues regulate function of living cells. The Axio Observer 7 with mechanobiology apabilities is a critical instrument for a diverse and growing number of biomedical researchers at Boise State University. These researchers include biomedical engineers, biomechanists, and biologists from three colleges: College of Engineering, College of Health Sciences, and College of Arts and Sciences. A unifying theme of this group’s research is the investigation of mechanically active musculoskeletal systems and materials in normal, diseased, and treated states. With strategic investments by Boise State University, this group has rapidly grown in the last 10 years (7 new faculty, 5 new labs), creating a critical mass of complementary expertise that is positioned to collaborate on transformative and synergistic orthopaedic and musculoskeletal research. Yet achieving this potential requires the acquisition of state-of-the-art equipment to investigate the physiological and pathophysiological processes from molecular to tissue scales. Our ability to link this structural hierarchy is currently limited by the absence of a system that can apply physiologically relevant forces with a capability to observe molecular and mechanical consequences. As a result, biomedical faculty at Boise State University are poorly equipped to transition from discoveries in basic science to innovations with direct clinical application related to musculoskeletal health. Therefore there is a clear benefit of this live-cell imaging and mechanosignaling capabilities offered by this microscope system. The acquisition of the Axio Observer 7 with mechanobiology capabilities will correct this deficiency and will not only benefit NIH funded current project but also spur the initiation of many impactful projects with high funding potential.

项目摘要 该提案的目的是获取Zeiss Axio观察者7 Live Cell成像系统，该系统将耦合 使用可用的定制和商业生物反应器系统，可以以生物医学研究为重点 关于Boise State的研究人员的变形显微镜，机械适应和细胞跟踪接近者 以及邻近和合作者机构。 Matrix Biology和Idaho生物医学研究卓越网络（Inbrew）的卓越（COBRE） 是博伊西州生物力学和机械生物学的生物医学研究的快速增长。 建立博士 材料工程和生物分子科学的计划，使生物学的重点增加了 尽管有这种增长，但仍有一个共识显微镜为博伊西州的所有研究人员提供服务。 这不是Onle botleneck可以使用更适合3D应用程序的共聚焦系统的使用，但也限制了 机械方法研究活细胞的细胞外提示正规功能。 使用机械生物学的能力是多种生物医学数量的重要工具 博伊西州立大学的研究人员包括生物医学工程师，生物力学和 三个学院的生物学家：卫生学院工程学院。 科学。 Boise State的正常，患病和治疗状态的系统和材料。 大学，该小组在过去的10年中迅速发展（7个新教师，5个新实验室），创造了一个临界质量 互补专业知识的合作，可以在变革性和协同的骨根上进行合作 肌肉骨骼研究。 研究从分子到组织量表的生理和病理生理过程。 该结构层次结构目前受到无法应用生理学相关的系统的限制 有能力观察分子和机械后果。 博伊西州立大学从基础科学的发现过渡到与 与肌肉骨骼健康有关的直接临床应用。 该显微镜系统提供的成像和机械信号能力。 具有机械生物学功能的观察者7将纠正这一点，并且不会。 当前的项目，但也促使启动许多具有高资金潜力的Impactivel项目。