Sartorious Octet R8 System

赛多利斯八位位组 R8 系统

• 批准号: 10429632
• 负责人: Manish Sagar
• 金额: $ 24.91万
• 依托单位: BOSTON MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-17 至 2023-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429632
• 关键词: Advisory Committees; Antibodies; Autoimmune Diseases; Basic Science; Binding; Biological Assay; Boston; Cardiovascular Diseases; Clinical Research; Communicable Diseases; Communities; Diabetes Mellitus; Diagnosis; Education; Equipment; Event; Fc Receptor; Feedback; Financial Support; Funding; Goals; Grant; Heart Diseases; In Vitro; Individual; Industry; Institution; Interferometry; Investments; Kidney Diseases; Kinetics; Maintenance; Medical; Medical center; Mission; Modernization; Monitor; Obesity; Pathogenesis; Peptides; Price; Progress Reports; Proteins; Publications; Reporting; Research; Research Personnel; Scientist; Services; System; Time; Training; Translational Research; United States National Institutes of Health; Universities; Virus; base; cost; drug development; experience; human disease; instrument; instrumentation; novel; pandemic disease; programs; small molecule; targeted treatment; vaccine development

PROJECT SUMMARY / ABSTRACT The proposed S10 project entitled “Octet R8 System for Molecular Interaction Studies in Infectious and Non-infectious Diseases” is to respond to PAR-21-125 Basic Instrumentation Grant (BIG) Program. The mission of the Analytical Instrumentation Core (AIC) at Boston Medical Center (BMC) and Boston University (BU) is to provide support and training to investigators through cutting-edge analytical instruments and services to facilitate research and education. The ultimate goal is to contribute to the understanding of the pathogenesis, diagnosis, and treatment of a diverse range of human diseases. The AIC meets this goal by providing access to modern, sophisticated high-priced instruments that are necessary to advancing the research of multiple biomedical investigators. These instruments are typically cost-prohibitive for individual scientists to purchase, operate, and maintain. Based on feedback from our investigators, having highly sensitive approaches for identifying and quantifying intramolecular interactions between antibodies, receptors, peptides, small molecules, and viruses have consistently been reported as an AIC service of the greatest need. This is especially relevant because many groups have started more translational projects over the past several years (particularly because of the current pandemic) and require the ability to quantitatively detect binding between novel targets and therapeutics. This desire from multiple investigators has led us to explore the possibility of purchasing a Sartorius 8-channel Octet biolayer interferometry (BLI) R8 system. Industry- leading BLI analytical instruments precisely and accurately monitor binding between two different molecules in real-time. The Octet R8 will provide investigators a highly sensitive and reliable way to conduct binding assays, to quantify proteins, antibodies, peptides and small molecules, and to directly monitor the kinetics of binding events and interactions between biomolecules from in-vitro and ex-vivo studies. The AIC provides services to more than 150 investigators/labs, and has over 10 NIH funded projects that would directly utilize the Octet R8. Presence of BLI services will allow researchers to perform their projects with greater precision and sensitivity. We propose that the Octet-R8 will be housed at the AIC, which is located on the BU Medical Campus. However, it will still be equally accessible to investigators across the entire research community. This will be highly beneficial because the AIC has extensive experience and a magnificent record of accomplishment regarding instrument maintenance and training users. The instrument will be assured to be in excellent working condition, thus minimizing any potential down time. The institution will provide substantial financial support and there is also an advisory committee to assist with biosafety needs, publications, and annual progress reports. Investment in this equipment will allow our investigators to conduct studies of high significance for basic, translational and clinical research into infectious diseases, vaccine development, diabetes, heart disease, obesity, kidney disease, autoimmune diseases, cardiovascular diseases, and drug development.

项目概要/摘要 拟议的 S10 项目题为“传染病和分子相互作用研究的 Octet R8 系统” 非传染性疾病”是为了响应 PAR-21-125 基本仪器补助金 (BIG) 计划。 波士顿医学中心 (BMC) 和波士顿大学分析仪器核心 (AIC) 的使命 （BU）是通过尖端的分析仪器和服务为研究人员提供支持和培训 促进研究和教育的最终目标是促进对事物的理解。 AIC 通过对多种人类疾病的发病机制、诊断和治疗来实现这一目标。 提供使用现代、先进、高价仪器的机会，这些仪器对于推进 多个生物医学研究人员的研究对于个人来说通常成本高昂。 根据我们研究人员的反馈，科学家购买、操作和维护具有高度的评价。 识别和量化抗体、受体之间分子内相互作用的灵敏方法 肽、小分子和病毒一直被报道为最伟大的 AIC 服务 这尤其重要，因为许多团体过去已经启动了更多的转化项目。 几年（特别是由于当前的大流行）并且需要能够定量检测 多个研究人员的这种愿望促使我们探索新靶点和治疗方法之间的结合。 可以购买 Sartorius 8 通道 Octet 生物层干涉测量 (BLI) R8 系统 Industry-。 领先的 BLI 分析仪器精确监测两种不同分子之间的结合 Octet R8 将为研究人员提供一种高度灵敏且可靠的方法来进行结合测定， 定量蛋白质、抗体、肽和小分子，并直接监测结合动力学 AIC 为体外和离体研究中的生物分子之间的事件和相互作用提供服务。 超过 150 名研究人员/实验室，并有超过 10 个 NIH 资助的项目将直接利用 Octet R8。 BLI 服务的存在将使研究人员能够以更高的精度和灵敏度执行他们的项目。 我们建议将 Octet-R8 安置在位于 BU 医学园区的 AIC 内。 然而，整个研究界的研究人员仍然可以平等地访问它。 非常有益，因为 AIC 拥有丰富的经验和辉煌的成就记录 关于仪器维护和培训用户，将确保仪器良好工作。 条件，从而最大限度地减少任何潜在的停机时间。该机构将提供大量的财政支持和 还有一个咨询委员会，负责协助满足生物安全需求、出版物和年度进展报告。 对该设备的投资将使我们的研究人员能够进行对基础、 传染病、疫苗开发、糖尿病、心脏病、 肥胖、肾脏疾病、自身免疫性疾病、心血管疾病和药物开发。