Sartorious Octet R8 System

赛多利斯八位位组 R8 系统

• 批准号: 10429632
• 负责人: Manish Sagar
• 金额: $ 24.91万
• 依托单位: BOSTON MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-17 至 2023-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429632
• 关键词: Advisory Committees; Antibodies; Autoimmune Diseases; Basic Science; Binding; Biological Assay; Boston; Cardiovascular Diseases; Clinical Research; Communicable Diseases; Communities; Diabetes Mellitus; Diagnosis; Education; Equipment; Event; Fc Receptor; Feedback; Financial Support; Funding; Goals; Grant; Heart Diseases; In Vitro; Individual; Industry; Institution; Interferometry; Investments; Kidney Diseases; Kinetics; Maintenance; Medical; Medical center; Mission; Modernization; Monitor; Obesity; Pathogenesis; Peptides; Price; Progress Reports; Proteins; Publications; Reporting; Research; Research Personnel; Scientist; Services; System; Time; Training; Translational Research; United States National Institutes of Health; Universities; Virus; base; cost; drug development; experience; human disease; instrument; instrumentation; novel; pandemic disease; programs; small molecule; targeted treatment; vaccine development

PROJECT SUMMARY / ABSTRACT The proposed S10 project entitled “Octet R8 System for Molecular Interaction Studies in Infectious and Non-infectious Diseases” is to respond to PAR-21-125 Basic Instrumentation Grant (BIG) Program. The mission of the Analytical Instrumentation Core (AIC) at Boston Medical Center (BMC) and Boston University (BU) is to provide support and training to investigators through cutting-edge analytical instruments and services to facilitate research and education. The ultimate goal is to contribute to the understanding of the pathogenesis, diagnosis, and treatment of a diverse range of human diseases. The AIC meets this goal by providing access to modern, sophisticated high-priced instruments that are necessary to advancing the research of multiple biomedical investigators. These instruments are typically cost-prohibitive for individual scientists to purchase, operate, and maintain. Based on feedback from our investigators, having highly sensitive approaches for identifying and quantifying intramolecular interactions between antibodies, receptors, peptides, small molecules, and viruses have consistently been reported as an AIC service of the greatest need. This is especially relevant because many groups have started more translational projects over the past several years (particularly because of the current pandemic) and require the ability to quantitatively detect binding between novel targets and therapeutics. This desire from multiple investigators has led us to explore the possibility of purchasing a Sartorius 8-channel Octet biolayer interferometry (BLI) R8 system. Industry- leading BLI analytical instruments precisely and accurately monitor binding between two different molecules in real-time. The Octet R8 will provide investigators a highly sensitive and reliable way to conduct binding assays, to quantify proteins, antibodies, peptides and small molecules, and to directly monitor the kinetics of binding events and interactions between biomolecules from in-vitro and ex-vivo studies. The AIC provides services to more than 150 investigators/labs, and has over 10 NIH funded projects that would directly utilize the Octet R8. Presence of BLI services will allow researchers to perform their projects with greater precision and sensitivity. We propose that the Octet-R8 will be housed at the AIC, which is located on the BU Medical Campus. However, it will still be equally accessible to investigators across the entire research community. This will be highly beneficial because the AIC has extensive experience and a magnificent record of accomplishment regarding instrument maintenance and training users. The instrument will be assured to be in excellent working condition, thus minimizing any potential down time. The institution will provide substantial financial support and there is also an advisory committee to assist with biosafety needs, publications, and annual progress reports. Investment in this equipment will allow our investigators to conduct studies of high significance for basic, translational and clinical research into infectious diseases, vaccine development, diabetes, heart disease, obesity, kidney disease, autoimmune diseases, cardiovascular diseases, and drug development.

项目摘要 /摘要 拟议的S10项目标题为“用于传染和 非感染性疾病”是为了应对PAR-21-125基本仪器赠款（BIG）计划。 波士顿医学中心（BMC）和波士顿大学的分析仪器核心（AIC）任务 （BU）是通过尖端的分析工具和服务向调查人员提供支持和培训 促进研究和教育。最终目标是为理解 潜水员人类疾病的发病机理，诊断和治疗。 AIC符合这个目标 提供获得现代，精致的高价乐器的访问权限 多个生物医学研究人员的研究。这些仪器通常对个人来说是成本良好的 科学家购买，运营和维护。基于我们调查人员的反馈 识别和量化抗体，接收器，，接收器之间分子内相互作用的敏感方法 肽，小分子和病毒一直被报道为最伟大的AIC服务 需要。这尤其重要，因为许多小组过去已经开始了更多的翻译项目 几年（部分是由于当前大流行），需要定量检测的能力 新型靶标和治疗之间的结合。多个调查人员的愿望使我们探索了 购买sartorius 8通道八八角材料干扰（BLI）R8系统的可能性。行业- 领先的BLI分析仪器精确，准确地监测两个不同分子之间的结合 即时的。八位位r8将为调查人员提供一种高度敏感和可靠的方法来进行绑定测定， 量化蛋白质，抗体，胡椒粉和小分子，并直接监测结合的动力学 来自体外和体外研究的生物分子之间的事件和相互作用。 AIC为 超过150名调查人员/实验室，拥有10多个NIH资助的项目，这些项目将直接利用八位八月R8。 BLI服务的存在将使研究人员能够以更高的精度和敏感性进行项目。 我们建议将八位钟r8安置在位于BU医疗校园的AIC。 但是，整个研究界的调查人员仍然可以使用它。这将是 非常有益，因为AIC具有丰富的经验和宏伟的成就记录 关于仪器维护和培训用户。该乐器将被认为是出色的工作 条件，从而最大程度地减少任何潜在的停机时间。该机构将提供大量财政支持，并 还有一个咨询委员会，以协助满足生物安全需求，出版物和年度进度报告。 对该设备的投资将使我们的调查人员能够对基本的研究具有很高的意义， 转化和临床研究对传染病，疫苗发育，糖尿病，心脏病， 肥胖，肾脏疾病，自身免疫性疾病，心血管疾病和药物发育。