Pro-inflammatory activation of human macrophages regulated by lncRNAs

lncRNA调控人巨噬细胞的促炎激活

基本信息

批准号：
10428357
负责人：
Masanori Aikawa
金额：
$ 72.36万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10428357
关键词：
Address Biological Biological Process Biology Blood Blood Vessels Cells Communities Complex Computational Biology Computer Analysis Coronary Arteriosclerosis Data Development Discipline Disease Drug usage Endotoxemia Event Gene Expression Profiling Goals Hematopoietic Stem Cell Transplantation Heterogeneity Human In Vitro Inflammation Inflammatory Laboratories Lesion Leukocytes Life Link Machine Learning Macrophage Activation Mechanics Mediating Methods Molecular Myocardial NF-kappa B Network-based Outcome Pathway Analysis Patients Plasma Play Protein Analysis Proteins Proteomics RNA Reporting Residual state Risk Risk Factors Role Science Signal Transduction Small Interfering RNA Splenocyte System Systems Biology Tissues Untranslated RNA Validation Vascular Diseases arterial lesion base cytokine experimental study femoral artery gain of function global health human disease humanized mouse in vivo injured loss of function macrophage modifiable risk mouse model multiple omics network models novel novel therapeutics overexpression protein protein interaction single cell analysis tool transcriptome transcriptome sequencing transcriptomics vascular inflammation

项目摘要

Project Summary Macrophage activation promotes major inflammatory disorders, including arterial diseases. Its underlying mechanisms, however, remain obscure. The present study will establish a systems approach, involving computational prediction analyses, multi-omics, network science, and in vitro and in vivo validation, to discover long noncoding RNA (lncRNA)-mediated mechanisms for pro-inflammatory activation of macrophages and arterial disease. In Specific Aim 1, we will involve omics studies of human macrophages to identify lncRNAs and their interacting proteins and develop computational analyses to predict human lncRNAs that regulate macrophage activation. Specific Aim 2 will examine the functionality of candidate lncRNAs in macrophage activation in vitro and in vivo. The findings from the study will help to identify new mechanisms for macrophage activation and may provide molecular bases for new therapies.

项目摘要巨噬细胞激活会促进包括动脉疾病在内的主要炎症性疾病。它的基础但是，机制仍然晦涩难懂。本研究将建立系统方法，涉及计算预测分析，多词，网络科学以及体外和体内验证发现长的非编码RNA（LNCRNA）介导的机制，用于促炎的激活巨噬细胞和动脉疾病。在特定的目标1中，我们将涉及人类巨噬细胞的法学研究鉴定lncRNA及其相互作用的蛋白质并开发计算分析以预测人类调节巨噬细胞激活的lncRNA。特定目标2将检查候选人的功能巨噬细胞在体外和体内激活中的LNCRNA。研究结果将有助于确定新的巨噬细胞激活的机制，可以为新疗法提供分子碱基。