Ultramicrotome with necessary accessories

超薄切片机及必要配件

• 批准号: 10416530
• 负责人: MARTIN L KATZ
• 金额: $ 15.16万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10416530
• 关键词: 3-Dimensional; Address; Age; Amyotrophic Lateral Sclerosis; Array tomography; Awareness; Biomedical Research; Cellular Structures; Confocal Microscopy; Connective Tissue Diseases; Core Facility; Cytology; Diamond; Disease; Dry Eye Syndromes; Electron Microscopy; Electrons; Equipment; Funding; Glass; Glean; Hereditary Disease; Image; Immunofluorescence Immunologic; Impairment; Individual; Inherited; Investigation; Laboratories; Light; Lysosomal Storage Diseases; Malignant Neoplasms; Microscope; Microscopic; Microscopy; Microtomy; Molecular; Nerve Degeneration; Pathogenesis; Research; Research Personnel; Retina; Retinal Degeneration; Services; Structure; Synapses; Techniques; Three-Dimensional Image; Tissue imaging; Tissues; Training; Universities; Work; digital imaging; effective therapy; image processing; instrument; instrumentation; interest; light microscopy; member; retinal neuron; vibration

Project Summary/Abstract Funds are requested to purchase a Leica ARTOS ultramicrotome with necessary accessories including a vibration isolation instrument table, diamond knives, and a glass knifemaker. Included with the purchase will be training for the major users and the core facility staff member responsible for overseeing use of the instrument. The instrument will be housed in the campus Molecular Cytology Core Facility (MCC) where it will replace an ultramicrotome that is over 30 years old and in disrepair. The existing instrument can no longer be serviced due to its age and lack of replacement parts. The ultramicrotome is essential for the research being conducted by members of a large user group. Research by the users that requires this instrument includes studies on hereditary neurodegenerative and retinal degenerative diseases, amyotrophic lateral sclerosis, dry eye diseases, connective tissue disorders, lysosomal storage diseases and cancer. The new instrument will enable the investigators to prepare tissue sections for both light and electron microscopic (EM) examination. The instrument will enable users to produce ribbons of serial sections that can be used to generate 3- dimensional images of tissues and cellular structures of interest using advanced light microscopy equipment (including confocal microscopes) at the MCC and electron microscopy capabilities of the University Electron Microscopy Core Facility (EMC) to obtain images for array tomography. The 3-D images will enable the investigators to address important research questions that cannot be addressed using microtomy instrumentation currently available at the University. For example, it appears that impaired retinal function in several hereditary disorders under investigation may be due to alterations in the synapses between retinal neurons. It is very difficult to determine whether this is the case by examining individual thin sections with TEM or with immunofluorescence (IF) using confocal microscopy alone. If the users can collect serial sections encompassing entire synaptic structures and generate 3-D composite images that can be examined with both EM and immunofluorescence, the users will be able to reliably determine whether there are disease-related structural alterations in the synapses that could explain the functional deficits. Numerous projects of anticipated users would benefit from having the capability of performing correlative IF and EM with array tomography. The information that users will be able to glean using the ultramicrotome will assist in developing better understanding of pathogenesis and in developing effective therapies for numerous disorders. In addition to benefiting the biomedical research of users that are identified in this proposal, the ultramicrotome has the potential to facilitate the research of additional potential biomedical researchers. Many laboratories utilize the MCC for a variety of microscopy techniques and digital image processing. Through their work in the MCC facility, they will become aware of the availability of the ultramicrotome and its capabilities and may elect to use it in their research.

项目摘要/摘要 要求资金购买带有必要配件在内的必要配件的Leica Artos Ultromicrotome 振动隔离仪表，钻石刀和玻璃刀具制造商。购买中包括 为主要用户和核心设施工作人员进行培训，负责监督使用 乐器。该仪器将安置在校园分子细胞学核心设施（MCC）中 更换超过30年历史且失修的超大核病。现有仪器不再是 由于其年龄和缺乏替换零件而服务。超小观机构对于研究是必不可少的 由大型用户组的成员进行。需要此工具的用户的研究包括 关于遗传神经退行性和视网膜退行性疾病，肌萎缩性侧索硬化症，干燥的研究 眼病，结缔组织疾病，溶酶体储存疾病和癌症。新工具将 使研究人员能够为光和电子显微镜检查（EM）检查准备组织切片。 该仪器将使用户能够生成串行部分的丝带，可用于生成3-- 使用高级光学显微镜设备的组织和感兴趣的细胞结构的尺寸图像 （包括共聚焦显微镜）在大学电子的MCC和电子显微镜功能上 显微镜核心设施（EMC）以获得阵列层析成像的图像。 3-D图像将使 研究人员解决了无法使用微切开术解决的重要研究问题 该大学目前可用的仪器。例如，看来视网膜功能受损 正在调查的几种遗传性疾病可能是由于视网膜之间的突触改变 神经元。很难通过使用tem检查单个薄部分来确定这种情况 或单独使用共聚焦显微镜使用免疫荧光（IF）。如果用户可以收集串行部分 涵盖整个突触结构并生成3-D复合图像，这两者都可以检查 EM和免疫荧光，用户将能够可靠地确定是否存在与疾病有关的 突触的结构变化，可以解释功能缺陷。许多预期的项目 用户将受益于具有与阵列断层扫描的IF和EM具有相关性的能力。这 用户将能够使用超小感蛋糕收集的信息将有助于更好地开发 了解发病机理和开发许多疾病的有效疗法。此外 从本提案中确定的用户的生物医学研究中受益，超大型机构具有 潜在的促进其他潜在生物医学研究人员的研究。许多实验室都利用 MCC用于多种显微镜技术和数字图像处理。通过他们在MCC的工作 设施，他们将意识到超级机体及其能力的可用性，并可能选择使用 它在他们的研究中。