Spinning-Disk Confocal Microscope for Wide-Field, Super-Resolution, and Live-Cell Imaging

用于宽视场、超分辨率和活细胞成像的转盘共焦显微镜

• 批准号: 10415611
• 负责人: Peter G Smith
• 金额: $ 60万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2023-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415611
• 关键词: 3-Dimensional; Area; Autophagocytosis; Axon; Cilia; Computer software; Confocal Microscopy; Coupled; Cytoskeleton; Elements; Funding; Growth; Histologic; Image; Image Analysis; Kansas; Lasers; Medical center; Microscope; Minor; Mitochondria; Optics; Phototoxicity; Preparation; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Senior Scientist; Signal Transduction; System; Technical Expertise; Time; United States National Institutes of Health; Universities; design; digital; exosome; imaging facilities; interest; lightspeed; live cell imaging; programs; solid state; trafficking

Project summary This application requests funding for the purchase a Nikon CSU-W1 SoRa Spinning-Disk Super-Resolution Confocal Microscope to support 28 NIH-Funded research projects by 16 major and 7 minor users at the University of Kansas Medical Center (KUMC). The requested system has four solid state lasers and a wide field of view and can image frame rates up to 200fps. The imaging speed and light efficiency of the CSU- W1 SoRa equipped with an environmental chamber and piezoelectric stage make it ideal for live cell imaging, an area of growing interest at KUMC. The increased optical resolution afforded by the microlensing of the SoRa unit together with the additional 3D deconvolution package will enhance spatial resolution capabilities to 120nm with minimal phototoxicity, and will benefit a number of investigators interested in capturing intracellular dynamics in a variety of subcellular systems (e.g., mitochondria, cytoskeleton, exosomes, vesicular trafficking, autophagy, cell-signaling, axonal remodeling, and primary cilia). No other spinning-disk confocal microscopes are present on the KUMC campus, and recent decommissioning of several obsolete confocal systems has led to a critical need. This system will be housed in space designed for and dedicated to confocal microscopy. The CSU-W1 SoRa will be integrated into our long-established Imaging Facility, fully staffed by 2 senior scientists with extensive technical expertise in confocal microscopy and image analysis. Adjacent rooms provide dedicated space for administration, histological preparation, and digital processing on high content image analysis workstations with NIS-Elements software. The Imaging Facility has been successfully facilitating research at KUMC for over 40 years and currently serves about 140 active users. The CSU-W1 SoRa will provide tangible benefit to a large number of programs at a time when recent growth of NIH-funded research at KUMC, coupled with recent reductions confocal capacity, have led to a critical need.

项目摘要 该申请要求购买尼康CSU-W1 Sora旋转盘超级分辨率 共聚焦显微镜支持28个NIH资助的研究项目，由16个主要用户和7个未成年人用户 堪萨斯大学医学中心（KUMC）。请求的系统具有四个固态激光器和宽阔的系统 视野和可以为200fps的帧速率映像。 CSU-的成像速度和光效率 W1 Sora配备了环境室和压电阶段，使其非常适合活电池 成像，在KUMC越来越感兴趣的领域。微透镜提供的光学分辨率增加 Sora单元以及附加的3D反卷积套件将增强空间分辨率 具有最低光毒性的功能至120nm，并将受益于许多对 在多种亚细胞系统中捕获细胞内动力学（例如，线粒体，细胞骨架， 外泌体，囊泡运输，自噬，细胞信号，轴突重塑和原发性纤毛）。没有其他 KUMC校园中存在旋转盘共聚焦显微镜，最近退役 几个过时的共聚焦系统导致了巨大的需求。该系统将安装在设计的空间中 用于并专用于共聚焦显微镜。 CSU-W1 Sora将集成到我们悠久的 成像设施，由2位高级科学家组成，具有广泛的共聚焦显微镜技术专长 和图像分析。相邻的房间提供了专门的管理空间，组织学准备， 以及使用NIS元素软件上的高内容图像分析工作站上的数字处理。这 成像设施已成功地在KUMC进行了40多年的研究，目前服务 大约有140个活跃用户。 CSU-W1 Sora将为大量计划提供切实的好处 NIH资助研究在KUMC的最新增长以及最近的降低共焦 能力，导致了巨大的需求。