Development of multifunctional resins for robust dentin bonding

开发用于牢固牙本质粘合的多功能树脂

基本信息

批准号：
10412961
负责人：
YONG WANG
金额：
$ 36.44万
依托单位：
UNIVERSITY OF MISSOURI KANSAS CITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10412961
关键词：
Acids Address Adhesives Age Bacteria Binding Biodegradation Chemistry Collagen Collagen Fibril Combinatorial Synthesis Dental Dental Enamel Dental caries Dentin Development Elasticity Engineering Enzymes Excision Exhibits Failure Frequencies General Population Gingiva Goals Hybrids Hydrolysis In Situ In Vitro Infiltration Life Link Liquid substance Longevity Matrix Metalloproteinases Operative Dentistry Oral Patients Penetration Persons Phase Plant Resins Polymers Predisposition Public Health Quality of life Recurrence Replacement Therapy Resistance Risk Root Caries Services Site Structure Surface System Time Tooth Loss Tooth structure Translating base chemical bond clinically relevant cost crosslink demineralization design improved in vivo innovation interfacial mechanical properties microleakage monomer novel restoration restorative dentistry restorative material restorative resins seal sound

项目摘要

Development of Multifunctional Resins for Robust Dentin Bonding Project Summary The current dental restorations suffer from reduced longevity mainly due to interfacial breakdown/failures, which cause microleakage, sensitivity, recurrent caries, restoration removal/replacement and extensive loss of sound tooth structure. The breakdown has been linked to the failure of current bonding systems to develop a durable seal to dentin. Current dentin bonding strategies mainly rely on micromechanical retention between infiltrated resin and exposed collagen fibrils in the demineralized dentin layer. Strength of this interlocking or entanglement depends on the quality and longevity of both the infiltrated resin and collagen fibrils within the hybrid layer. There is substantial evidence to suggest that the quality of this layer is very poor, and the micromechanical binding mechanism is intrinsically problematic, does not provide a strong, tight, and durable seal between restorative material and dentin. Results from both in vitro and in vivo studies including ours have indicated that the following critical issues inhibit the formation of a durable bond when using current restorative bonding systems. These issues include poor/no interactions/bindings between infiltrated resin and collagen, poor quality of infiltrated resin (due to inadequate monomer/polymer conversion and hydrolysis), and degradation of acid-etched/unprotected collagen fibrils. The unprotected collagen undergoes degradation by exogenous bacteria and endogenous MMPs (which are activated immediately by acid etching), proceeding with hydrolysis of poor quality resins. It is nearly impossible to obtain strong and durable interface bonding without dramatic alterations in chemistry and/or bonding strategies. Clearly new chemistry and new bonding concept must be developed before a revolutionary improvement in dental restorations can be accomplished. In this application, we propose to develop novel monomers for robust, durable binding to address all the above issues. Such functional monomers will be designed to crosslink resin at one end and crosslink collagen fibrils at the other, not only stabilizing and increasing the longevity of both resin and collagen phases but also creating a tight, strong bond between resin polymer and dentin collagen. The approach is innovative since it represents the first systematic design with rationally engineered chemistry to simultaneously tackle all the three critical challenges afflicting current bonding systems. The overall hypothesis of this proposal is that new restorative resins formulated to induce collagen crosslinking, strong resin-collagen interactions and resin crosslinking will provide enhanced interfacial structural integrity and increased durability in the presence of clinically relevant dentin substrates. A combinatory approach/strategy together with in situ interfacial multi-scale characterization will be used in the studies. This approach will allow us to identify the most promising structures for the development of a biodegradation-resistant restorative resin that provides a durable, structurally integrated interfacial layer with clinically relevant dentin substrates.

开发用于稳健牙本质键合的多功能树脂项目摘要当前的牙科修复体的寿命缩短主要是由于界面崩溃/失败，会导致微孔，灵敏度，复发性龋齿，恢复恢复/置换和广泛的损失声音结构。分解已与当前键合系统的失败有关将耐用的密封到牙本质。当前的牙本质键合策略主要依赖于微机械浸润的树脂与脱矿化牙本质层中暴露的胶原原纤维之间的保留。力量这种互锁或纠缠取决于浸润的树脂的质量和寿命混合层内的胶原蛋白原纤维。有大量证据表明该层的质量非常差，微机械结合机制本质上是有问题的，不提供恢复材料和牙本质之间的坚固，紧密且耐用的密封。体外和体内的结果包括我们在内的研究表明，以下关键问题抑制了持久债券的形成使用当前的还原键合系统。这些问题包括差/无互动/绑定在浸润的树脂和胶原蛋白之间，浸润的树脂质量较差（由于单体/聚合物不足，转化和水解），以及酸蚀刻/未受保护的胶原蛋白原纤维的降解。未受保护的胶原蛋白通过外源细菌和内源性MMPS经历降解（它们被激活立即通过酸蚀刻），进行质量较差的树脂的水解。几乎不可能获得牢固而耐用的界面键合，而没有化学和/或粘合的戏剧性改变策略。显然，必须在革命性之前制定新的化学和新的键合概念可以改善牙科修复体。在此应用中，我们建议开发新颖可靠，耐用粘合的单体，以解决上述所有问题。这样的功能单体将是设计用于一端交联树脂，另一端交叉链接胶原原纤维在另一端，不仅稳定和提高树脂和胶原蛋白阶段的寿命，同时也建立了紧密而牢固的联系树脂聚合物和牙本质胶原蛋白。该方法具有创新性，因为它代表了第一个系统设计通过合理设计的化学，可以同时应对困扰的所有三个关键挑战当前的粘结系统。该提议的总体假设是，新的修复树脂制定为诱导胶原蛋白交联，较强的树脂 - 胶原蛋白相互作用和树脂交联将提供增强界面结构完整性和在临床相关的牙本质底物存在下的耐用性。组合方法/策略以及原位界面多尺度表征将用于研究。这种方法将使我们能够确定开发最有希望的结构耐生物降解的修复树脂，可提供耐用的结构整合的界面层临床相关的牙本质底物。