Shared Resource - Microscopy & Imaging Shared Resource

共享资源 - 显微镜

基本信息

批准号：
10400651
负责人：
MICHAEL D JOHNSON
金额：
$ 5.88万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-15 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10400651
关键词：
3-Dimensional Aging Animals Apoptosis Biological Assay Brightfield Microscopy Cancer Center Support Grant Cancer Control Cell surface Cells Code Color Comprehensive Cancer Center Computer software Confocal Microscopy Consultations Cultured Cells Cytogenetics Development Doctor of Philosophy Educational workshop Electron Microscope Electron Microscopy Embryo Experimental Designs Fluorescein-5-isothiocyanate Fluorescence Fluorescence Microscopy Fluorescence Recovery After Photobleaching Fluorescence Resonance Energy Transfer Glass Goals Hybrids Image Image Analysis In Situ Individual Inflammation Injections Interference Reflection Microscopy Investigational Therapies Laboratories Lasers Licensing Methods Microinjections Microscope Microscopy Microtus Mitosis Molecular Cytogenetics Mus NCI Center for Cancer Research Optics Organ Phase Photobleaching Reagent Research Research Personnel Resource Sharing Scanning Services Site Slide Speed Supervision System Three-Dimensional Image Time Tissue imaging Tissues Training Transmission Electron Microscopy Universities angiogenesis anticancer research assay development base beam-splitter cancer prevention cellular imaging charge coupled device camera confocal imaging contrast imaging culture plates design detection sensitivity digital exosome experimental study fluorescence lifetime imaging fluorescence microscope imaging modality imaging system instrument instrumentation lectures light microscopy malignant breast neoplasm member microscopic imaging migration molecular oncology multiphoton imaging photon-counting detector programs red fluorescent protein tissue culture tissue/cell culture transmission process tumor tumorigenesis

项目摘要

The Microscopy & Imaging Shared Resource (MISR) was established in 1989 to provide 1) access to an array of well-maintained instrumentation for Georgetown Lombardi Comprehensive Cancer Center (LCCC) investigators wishing to apply light microscopy and image analysis to cancer research and 2) the expertise, training and reagents necessary for investigators to make optimal use of the instruments available. The MISR provides instrumentation for bright-field microscopy, routine fluorescence microscopy and more advanced applications, including confocal microscopy, microinjection, total internal reflection microscopy (TIRF), multispectral emission imaging, fluorescence lifetime imaging, Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and deep tissue multiphoton imaging. Live cell, time-lapse imaging is possible using five different wide-field and confocal systems. Using MetaMorph v7.8.13 offline image analysis and Volicity v6.3.0 3D Image Analysis Software, MISR develops automated routines for users carrying out common assays, such as cell tracking, proliferation, studies of mitosis and apoptosis plus angiogenesis experiments. The MISR also supports cytogenetics and molecular cytogenetics services and provides the following: • Access to nine major digital microscope workstations, transmission electron microscope and stand-alone image analysis workstations. It also supports 16 image analysis software licenses in individual laboratories. • Expert advice, training and assistance with sophisticated microscopy and image analysis approaches in cancer research. Since the last submission, the MISR 1) replaced an aging confocal system with a new highly capable Leica SP8 AOBS laser scanning confocal microscope, 2) upgraded the TIRF system with a new camera and a coded stage that extends the imaging modalities available and 3) currently supports multiple high-impact studies demonstrating the interaction between distinct cell surface molecules in tumorigenesis and inflammation. The MISR Director, M. Johnson, PhD, leads a team with a wealth of expertise in light and electron microscopy plus image analysis. In 2017, 20 LCCC members representing all four Research Programs (Breast Cancer [BC], Cancer Prevention and Control [CPC], Experimental Therapeutics [ET], and Molecular Oncology [MO]) used the MISR.

显微镜和成像共享资源（MISR）于1989年建立以提供1）访问一组乔治敦伦巴第综合癌症中心（LCCC）调查人员维护良好的仪器希望将光学显微镜和图像分析应用于癌症研究以及2）专业知识，培训和试剂调查人员最佳使用可用的仪器所必需的。 MISR提供仪器对于明亮的现场显微镜，常规荧光显微镜和更高级的应用，包括共焦显微镜，显微注射，总内反射显微镜（TIRF），多光谱发射成像，荧光寿命成像，Förster共振能量转移（FRET），荧光恢复后光漂白（FRAP）和深层组织多光子成像。实时单元，使用五个可以进行延时成像不同的宽场和共聚焦系统。使用Metamorph v7.8.13脱机图像分析和VoliCity v6.3.0 3D 图像分析软件，MISR为执行常见测定的用户开发自动例程，例如单元格跟踪，增殖，有丝分裂和凋亡以及血管生成实验的研究。 MISR也支持细胞遗传学和分子细胞遗传学服务，并提供以下内容： •访问九个主要的数字显微镜工作站，传输电子显微镜和独立的图像分析工作站。它还支持单个实验室中的16个图像分析软件许可。 •专家建议，培训和协助以复杂的显微镜和图像分析方法癌症研究。自上次提交以来，MISR 1）用新的功能强大的Leica SP8代替了老化的共聚焦系统 AOBS激光扫描共聚焦显微镜，2）用新相机和编码阶段升级了TIRF系统扩展了可用的成像方式，3）当前支持多个高影响研究证明了肿瘤发生和炎症中不同细胞表面分子之间的相互作用。 MISR主任M. Johnson博士领导一个具有丰富专业知识的团队图像分析。 2017年，有20名代表所有四个研究计划的LCCC成员（乳腺癌[BC]，癌症预防和对照[CPC]，实验疗法[ET]和分子肿瘤[MO]）使用了 MISR。