Discovery of Molecular Probes and Therapeutic Leads, Administrative Supplement for Equipment

分子探针和治疗先导物的发现，设备管理补充

基本信息

批准号：
10400299
负责人：
JOSEPH M FOX
金额：
$ 25万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10400299
关键词：
Adjuvant Administrative Supplement Analytical Chemistry Award Biochemistry Biological Biology Capsid Cells Centers of Research Excellence Chemicals Collaborations Communicable Diseases Complement Core Facility Custom Delaware Disease Engineering Enzymes Equipment Extramural Activities Faculty Faculty Recruitment Funding Goals Grant HIV Human Human Biology Immune response Infrastructure Kinetics Laboratories Legionnaires&apos Disease Lung Macromolecular Complexes Malignant Neoplasms Mass Spectrum Analysis Mentors Messenger RNA Microscopy Molecular Molecular Probes Molecular Target Pathway interactions Phase Pilot Projects Regulation Research Research Personnel Resource Sharing Services Structure Therapeutic Tuberculosis Universities Virus base catalyst cohort demethylation design human disease instrumentation interest member multidisciplinary nanoparticle nucleotide analog pathogenic bacteria professor programs recruit screening small molecule success tool vaccine development

Adjuvant Administrative Supplement Analytical Chemistry Award Biochemistry Biological Biology Capsid Cells Centers of Research Excellence Chemicals Collaborations Communicable Diseases Complement Core Facility Custom Delaware Disease Engineering Enzymes Equipment Extramural Activities Faculty Faculty Recruitment Funding Goals Grant HIV Human Human Biology Immune response Infrastructure Kinetics Laboratories Legionnaires&apos Disease Lung Macromolecular Complexes Malignant Neoplasms Mass Spectrum Analysis Mentors Messenger RNA Microscopy Molecular Molecular Probes Molecular Target Pathway interactions Phase Pilot Projects Regulation Research Research Personnel Resource Sharing Services Structure Therapeutic Tuberculosis Universities Virus base catalyst cohort demethylation design human disease instrumentation interest member multidisciplinary nanoparticle nucleotide analog pathogenic bacteria professor programs recruit screening small molecule success tool vaccine development

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项目摘要

Project Summary The goal of this Center of Biomedical Research Excellence is to continue our efforts to develop molecular approaches for probing biology, to discover and apply new chemical biology tools for the study of biological pathways associated with disease, and to develop computational approaches for understanding small molecule interactions with complex macromolecular targets. With core strengths in synthesis, biochemistry, engineering and biology, our scientific team will develop new chemical biology tools for understanding the cellular and molecular mechanisms that bacterial pathogens use to survive and replicate inside host cells, new chemical tools and nucleotide analogs to understand the regulation of mRNA demethylation by a ‘methyl eraser’ enzyme and its dysregulation in human cancer, molecularly-defined nanoparticle adjuvants that yield specific immune responses in the lung to advance inhalable vaccine development, and computational approaches to elucidate the mechanisms by which small molecules regulate the structure and function of virus capsids. COBRE funding will be used to recruit two new faculty members who will complement the existing strengths of our center, which will be expanded through competitive pilot grants. In years 4 and 5 of the COBRE, five two- year replacement projects will be funded. Collaboration and resource sharing in the Delaware and national IDeA network will be critical for maximizing our research capacity, core facilities and mentoring network. In Phase II, this COBRE continue to develop unique core facilities that facilitates high throughput synthesis, purification, screening and analysis of chemical probes. Building on the infrastructure established in Phase I, we will support a core facility in Analytical Chemistry, which will provide support for critical core facilities in mass spectrometry, solution NMR and kinetic analysis. The center will also support a Synthesis and Discovery core facility, which will provide custom synthesis services and instrumentation and infrastructure for small molecule synthesis, high throughput catalyst discovery, parallel synthesis, computation and microscopy.

项目摘要这个生物医学研究中心卓越的目标是继续我们的发展分子探测生物学，发现和应用新的化学生物学工具进行生物学研究的方法与疾病相关的途径，并开发计算方法来理解小的分子与复杂的大分子靶标的相互作用。具有合成，生物化学的核心优势，工程和生物学，我们的科学团队将开发新的化学生物学工具，以了解细菌病原体用于生存和复制宿主细胞的细胞和分子机制，新化学工具和核苷酸类似物，以了解通过'甲基对mRNA脱甲基化的调节橡皮擦的酶及其在人类癌症中的失调，分子定义的纳米颗粒调节器产生肺部的特定免疫反应以推动吸入疫苗的开发和计算阐明小分子调节病毒结构和功能的机制的方法衣壳。毛cr筹资金将用于招募两个新的教职员工，他们将完成现有的优势我们的中心将通过有竞争力的飞行员赠款扩展。在毛绒的4年级和第5年中，五个二 - 年度替代项目将被资助。特拉华州和国家的合作和资源共享创意网络对于最大化我们的研究能力，核心设施和心理网络至关重要。在第二阶段，这种毛绒继续开发独特的核心设施，以促进高通量合成，化学问题的纯化，筛查和分析。建立在第一阶段建立的基础设施的基础上我们将支持分析化学方面的核心设施，该设施将为关键的核心设施提供支持质谱法，溶液NMR和动力学分析。该中心还将支持合成和发现核心设施将为小型提供自定义合成服务以及仪器和基础设施分子合成，高吞吐量催化剂发现，平行合成，计算和显微镜。